We narrowed to 7,283 results for: Tet-on

1. pTS106 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-GFP

   Plasmid

   #199359

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-GFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-SUMOEu-EGFP

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE2-TetOminiCMV-Oct4-P2A-Klf4-IRES-Sox2-17

   Plasmid

   #210020

   Purpose

   tetO-OKS* (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + Klf4 + super-Sox)

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   Oct4-p2a-Klf4-IRES-Sox2-17 (Klf4, Pou5f1, Sox2 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TetO-mini-CMV

   Available Since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-TRE-DIO-eGFP-2A-TetanusToxin (Cre-OFF)

   Plasmid

   #166611

   Purpose

   Encodes Cre-inactivated GFP-2A-Tetanus toxin light chain under control of the TRE

   Depositor

   Martin Myers

   Article

   Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

   Insert

   EGFP-2A-Tetanus Toxin
   

   Use

   AAV

   Promoter

   tetracycline response element

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB

   Plasmid

   #78348

   Purpose

   Expresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

   Depositor

   Michael Elowitz

   Article

   Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

   Insert

   H2B-mCherry-T2A-rTetR-KRAB
   

   Use

   Synthetic Biology

   Tags

   rTetR

   Expression

   Mammalian

   Promoter

   pEF alpha

   Available Since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBabe-Sin-Puro-Tet-RhoA FLARE.sc Biosensor

   Plasmid

   #12153

   Depositor

   Klaus Hahn

   Article

   Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

   Insert

   RhoA Biosensor (RHOA Human)
   

   Use

   Retroviral

   Tags

   6XHis

   Expression

   Mammalian

   Available Since

   July 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [C12orf35]

   Plasmid

   #227245

   Purpose

   All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in CHO cell

   Depositor

   Jae Seong Lee

   Article

   Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.

   Inserts

   Tet-On 3G transactivator protein
   Enhanced green fluorescent protein
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF1α and pTRE3G

   Available Since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEx1-pEF-H2B-mCherry-T2A-rTetR-EED

   Plasmid

   #78101

   Purpose

   Expresses rTetR-EED for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

   Depositor

   Michael Elowitz

   Article

   Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

   Insert

   H2B-mCherry-T2A-rTetR-EED (EED Human, Synthetic)
   

   Use

   Synthetic Biology

   Tags

   rTetR

   Expression

   Mammalian

   Promoter

   pEF alpha

   Available Since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE2-TetOminiCMV-Sox2-17-t2a-Klf4-e2a-Myc

   Plasmid

   #193349

   Purpose

   tetO-S*KM (Dox-inducible reprogramming lentiviral vector expressing mouse super-Sox + Klf4 + cMyc)

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   Sox2-17-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Sox2-17 is engineered highly cooperative chimeric…

   Promoter

   TetO mini CMV

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. MF87_PB-EF1a-TET3G-P2A-ERT2-Gal4-BGHpA

   Plasmid

   #176630

   Purpose

   Integrated in CHO-K1 to make parental polyclonal lines for all other MultiFate cell lines

   Depositor

   Michael Elowitz

   Article

   Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

   Insert

   EF1a-TET3G-P2A-ERT2-Gal4-BGHpA
   

   Use

   Synthetic Biology

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAV-H1promoter-2xTetO-High_ribosome_load-Tornado-2xALFA-10xSunTag

    Plasmid

    #231594

    Purpose

    Express high ribosome loading circular RNA translation reporter with 2xALFATag-10xSunTag under H1 promoter

    Depositor

    Marvin Tanenbaum

    Article

    Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

    Insert

    Kozak sequence-Tornado-10xSunTag-2xALFATag
    

    Expression

    Mammalian

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTS97 pHAGE2 CMVtetO2 GFP-22xGS-SUMOstar-MCS

    Plasmid

    #199350

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOstar-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP-SUMOstar

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTS95 pHAGE2 CMVtetO2 GFP-22xGS-TEV-MCS

    Plasmid

    #199348

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-TEV-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP-TEV

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4

    Plasmid

    #78349

    Purpose

    Expresses rTetR-HDAC4 for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

    Depositor

    Michael Elowitz

    Article

    Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

    Insert

    H2B-mCherry-T2A-rTetR-HDAC4 (HDAC4 Human, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    rTetR

    Expression

    Mammalian

    Promoter

    pEF alpha

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBS NL4-3 envFS eGFP 5'CMV Δtat ΔTARx2_d2TetOp

    Plasmid

    #101349

    Purpose

    2xTet Operator inserted between NFkB and Sp1 sites in U3 of HIV-1 delta tat with 5' CMV-R-U5. 5' and 3' TAR elements were mutated to: 5'-GGTCTCTCTGGTTAGACCAGAAAGGAGCATTGGAGCTCTCTGGCTAACTAGGGAACCC-3

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3
    

    Use

    Lentiviral

    Mutation

    delta tat delta TAR delta env GFP in Nef Tet ope…

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLQ7748 mU6: Spy sgTET || CMV: mCherry~P2A-DD-A4

    Plasmid

    #123657

    Purpose

    DD-AcrIIA4 fusion for Shield1-mediated AcrIIA4 control with sgRNA driving TRE3G activation.

    Depositor

    Stanley Qi

    Article

    Nakamura et al Nat Commun. 2019 Jan 14;10(1):194. doi:

    Insert

    mCherry-P2A-DD-AcrIIA4
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    mCherry-P2A

    Expression

    Mammalian

    Promoter

    CMV and mU6

    Available Since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiTet-RAB10-T2A-RABIF-P2A-mCherry BlastR

    Plasmid

    #186740

    Purpose

    Dox inducible RAB10-T2A-RABIF-P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

    Depositor

    Richard Sherwood

    Article

    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

    Insert

    RAB10, RABIF (RAB10, RABIF Human)
    

    Use

    Lentiviral

    Tags

    RAB10-T2A-RABIF-P2A-mCherry

    Expression

    Mammalian

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTS105 pHAGE2 CMVtetO2 MCS-TEV-22xGS-GFP

    Plasmid

    #199358

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-GFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TEV-EGFP

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHAGE2-TetOminiCMV-Oct4-P2A-Klf4-IRES-Sox2

    Plasmid

    #210019

    Purpose

    tetO-OKS (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + Klf4 + Sox2)

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    Oct4-p2a-Klf4-IRES-Sox2 (Klf4, Pou5f1, Sox2 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TetO-mini-CMV

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Exp-pcDNA3.2delCMV(EF1α-tTA/TetO-mCh-Rs1)

    Plasmid

    #26797

    Depositor

    Edward Hsiao

    Article

    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

    Insert

    EF1a-tTA/TetO-mCherry-P2A-Rs1 (HTR4 Human)
    

    Expression

    Mammalian

    Mutation

    Human 5HT4B receptor with D100A mutation, generat…

    Available Since

    Feb. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLB62_PB_pGK-H2B-mIFP-T2A-rTetR-humanKRAB-zeo

    Plasmid

    #179439

    Purpose

    Expresses rTetR-KRAB(human) for gene silencing, integration by Piggybac

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    H2B-mIFP-T2A-rTetR-KRAB
    

    Expression

    Mammalian

    Promoter

    pGK

    Available Since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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