We narrowed to 7,108 results for: Tet-on

1. pcDNA4-Tet-on-vsv-CenpB 1-166-dCEN- Incenp-GFP

   Plasmid

   #108484

   Purpose

   inducible expression of vsv-CenpB-1-166-dCEN-INCENP-EGFP

   Depositor

   Susanne Lens

   Article

   Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

   Insert

   CenpB-dCEN-INCENP (INCENP Human)
   

   Use

   Tags

   EGFP and VSV

   Expression

   Mammalian

   Mutation

   CEN box replaced by CenpB 1-166

   Promoter

   CMV

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBEST-p15a-pBAD-TetO1-UTR1-deGFP-ssrA-T500

   Plasmid

   #45788

   Depositor

   Richard Murray

   Insert

   deGFP
   

   Use

   Synthetic Biology

   Tags

   ssrA degradation tag

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD-tetO1

   Available since

   July 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRRL-pEF-H2B-mCherry-T2A-rTetR-DNMT3L-SV40

   Plasmid

   #186967

   Purpose

   Lentiviral vector encoding DNMT3A recruiter for methylation reporter (pEF-H2B-mCherry-T2A-rTetR-DNMT3L-SV40) for expression in mammalian cells.

   Depositor

   Brian Liau

   Article

   Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

   Insert

   DNMT3L (DNMT3L Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   rTetR fusion

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXTK-BB7 [Backbone_repABC-pMlb-KanR-TetR-PaqCI-Compatible_PartTypeBB]

   Plasmid

   #229393

   Purpose

   XanthoMoClo Golden Gate Backbone Plasmid, contains Backbone_repABC-pMlb-KanR-TetR-PaqCI-Compatible_PartTypeBB and an sfGFP dropout

   Depositor

   Michael Springer

   Article

   Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAV-H1promoter-2xTetO-Tornado-2xALFA-10xSunTag-Xbp1(S255A)

   Plasmid

   #231592

   Purpose

   Express circular RNA translation reporter with 2xALFATag-10xSunTag-Xbp1(S255A) pause site under H1 promoter

   Depositor

   Marvin Tanenbaum

   Article

   Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

   Insert

   Tornado-2xALFATag-10xSunTag-Xbp1(S255A) pause sequence
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCyno-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

   Plasmid

   #212830

   Purpose

   Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the cynomolgus genome.

   Depositor

   Wolfgang Enard

   Article

   Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

   Insert

   ZIM3-KRAB-dCas9-P2A-mCherry
   

   Use

   Tags

   HA-tag and ZIM3-KRAB

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG, TetOn

   Available since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGorilla-AAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry

   Plasmid

   #212831

   Purpose

   Doxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the gorilla genome.

   Depositor

   Wolfgang Enard

   Article

   Edenhofer et al iScience. 2024 May 23;27(6):110090. doi:

   Insert

   ZIM3-KRAB-dCas9-P2A-mCherry
   

   Use

   Tags

   HA-tag and ZIM3-KRAB

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG, TetOn

   Available since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFUW-tetO-mCh-Gata2-del-NLS(380-440)

   Plasmid

   #193070

   Purpose

   doxycycline-inducible expression of mouse Gata2 without the nuclear localisation signal (amino acids 380-440), in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

   Insert

   mCh-Gata2-del-NSL(380-440)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   minimal CMV

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAG306-pTet07-synYFP[TTG/AGA]-4xArg[CGA]-nLuc

   Plasmid

   #199759

   Purpose

   Elongation reporter construct to quantify elongation duration of 4_CGA stalling

   Depositor

   Brian Zid

   Article

   Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

   Insert

   synYFP[TTG/AGA]-4xArg[CGA]-nLuc
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TetO7

   Available since

   May 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAG306-pTet07-synYFP[TTG/AGA]-6xArg[CGA]-nLuc

    Plasmid

    #199760

    Purpose

    Elongation reporter construct to quantify elongation duration of 6_CGA stalling

    Depositor

    Brian Zid

    Article

    Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

    Insert

    synYFP[TTG/AGA]-6xArg[CGA]-nLuc
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TetO7

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAG306-pTet07-synYFP[TTG/AGA]-6xArg[AGA]-nLuc

    Plasmid

    #199761

    Purpose

    Elongation reporter construct to quantify elongation duration of 6_AGA stalling

    Depositor

    Brian Zid

    Article

    Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

    Insert

    synYFP[TTG/AGA]-6xArg[AGA]-cLuc
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TetO7

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tet-inducible 3xFlag Mouse C325A Caspase-9 ΔLD + ΔSS

    Plasmid

    #194610

    Purpose

    Inducible Expression of N-Terminal 3X Flag Mouse C325A Caspase-9 Minus Linker + Small Subunit Domain

    Depositor

    Hannah Rabinowich

    Article

    Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

    Insert

    N-terminal 3X Flag Mouse C325A Caspase-9 Minus Sequence Encoding Ala 354 to Ser 454 (Casp9 Mouse)
    

    Use

    Tags

    3X Flag

    Expression

    Mammalian

    Mutation

    Changed Cysteine 325 to Alanine to eliminate prot…

    Promoter

    CMV

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSL006_phiC31_neo-5xTetO-pEF-H2B-mCitrine-pRSV-H2B-mCherry

    Plasmid

    #179425

    Purpose

    Dual fluorescent reporter construct with no spacer, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    TRE-cit-(nospacer)-mch
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA4-Tet-on-vsv-Incenp d543-746iPRC1_279-482-GFP

    Plasmid

    #108500

    Purpose

    inducible expression of vsv-INCENP d543-746iPRC1 279-482-EGFP

    Depositor

    Susanne Lens

    Article

    van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

    Insert

    INCENP d543-746iPRC1 304-509 (PRC1 Human)
    

    Use

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    delta alpha Helix, insert PRC1 microtubule domain

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLentiRNACRISPR_008-TetO-NLS-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid

    #223173

    Purpose

    Expresses hfCas13d (N2V8) in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus.

    Depositor

    Neville Sanjana

    Article

    Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

    Insert

    hfCas13d (N2V8)
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE2-TetOminiCMV-Oct4-p2a-Sox2-17-t2a-Klf4-e2a-cMyc

    Plasmid

    #193346

    Purpose

    tetO-OS*KM (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + super-Sox + Klf4 + cMyc)

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    Oct4-p2a-Sox2-17-t2a-Klf4-e2a-cMyc (Klf4, Pou5f1, Sox2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Sox2-17 is engineered highly cooperative chimeric…

    Promoter

    TetO mini CMV

    Available since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid

    #138149

    Purpose

    Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    CasRx
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMH002_phiC31_neo-5xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

    Plasmid

    #179430

    Purpose

    Dual fluorescent reporter construct with single HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    TRE-cit-HS4-mch (SH)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJEP10-AAV-U6/TO-gRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid

    #82706

    Purpose

    AAV backbone with a full length U6/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

    Depositor

    Jonathan Ploski

    Article

    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

    Insert

    U6/TO Empty gRNA Expression Cassette
    

    Use

    AAV and CRISPR

    Tags

    CMV promoter driving expression of TetR-P2A-GFP-K…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSYN-DRD2-V2tail-TevN-BLITz1-TetR-VP16-bGHpA

    Plasmid

    #89874

    Purpose

    expresses DRD2-iTango component in mammalian cells for AAV production

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    DRD2-V2tail-TevN-BLITz1-TetR-VP16
    

    Use

    AAV

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    short isoform of DRD2 (deletion of 242-270)

    Promoter

    Human synapsin

    Available since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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