We narrowed to 2,278 results for: Tub;

1. pCMV-N2-6MYC-hMID2

   Plasmid

   #51035

   Purpose

   mammalian expression of myc-tagged human MID2

   Depositor

   Timothy Cox

   Article

   Short et al BMC Cell Biol. 2002;3:1. Epub 2002 Jan 4

   Insert

   MID2 (MID2 Human)
   

   Use

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX458-sgTSC2

   Plasmid

   #128098

   Purpose

   CRISPR gRNA against human TSC2 with Cas9 from S. pyogenes and 2A-EGFP

   Depositor

   Chi Van Dang

   Article

   Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

   Insert

   sgRNA against human TSC2 (TSC2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEB1N-LZ-LOV2(fast)

   Plasmid

   #107690

   Purpose

   N-terminal half of π-EB1 with fast LOV2, no fluorescent tag

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE1 (MAPRE1 Human)
   

   Use

   Tags

   A. sativa phototropin 1 LOV2 domain (I427V, H519L…

   Expression

   Mammalian

   Mutation

   EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…

   Promoter

   CMV

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. SITS-mCherry-Bin1b

   Plasmid

   #176025

   Purpose

   Leishmania cell free expression of zebrafish mCherry-Bin1b. Parton lab clone GSU

   Depositor

   Rob Parton

   Article

   Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

   Insert

   Bin1b (bin1b Zebrafish)
   

   Use

   Leishmania cell free expression

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTER RB1shRNA human

   Plasmid

   #66886

   Purpose

   Doxycycline-regulated mammalian expression vector for expressing shRNA against RB1

   Depositor

   Maria Alvarado-Kristensson

   Article

   Ehlen et al J Biol Chem. 2012 May 18;287(21):17241-7

   Insert

   retinoblastoma 1 (RB1 Human)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1 Tet

   Available since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pBrain-GFP-TACC3KDP(L566,567A)-shTACC3

   Plasmid

   #69117

   Purpose

   Dual-promoter plasmid to express knockdown-proof human TACC3 (L566A and L567A substitutions) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.

   Depositor

   Stephen Royle

   Article

   Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

   Inserts

   Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
   shTACC3
   

   Use

   RNAi

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   LL (566-567aa) mutated to AA. Silent mutations i…

   Promoter

   CMV

   Available since

   Feb. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cavin4a in pBluescript(SK+)

   Plasmid

   #126567

   Purpose

   For generating RNA probes for in situ hybridisation. Parton lab clone ISB

   Depositor

   Rob Parton

   Article

   Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

   Insert

   Cavin4a (cavin4a Zebrafish)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. PC4_3spc34h

   Plasmid

   #70700

   Purpose

   Expresses S. cerevisiae DASH/Dam1 complex with C-terminal his-tag on spc34

   Depositor

   Stephen Harrison

   Article

   Miranda et al Nat Struct Mol Biol. 2005 Feb;12(2):138-

   Insert

   DASH / Dam1 complex (DAM1, ASK1, DUO1, SPC34, SPC19, DAD1, DAD2, DAD4, HSK3, DAD3 Budding Yeast)
   

   Use

   Tags

   his-tag on C-terminus of spc34

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. PC4_3dad1h

   Plasmid

   #70699

   Purpose

   Expresses S. cerevisiae DASH/Dam1 complex with C-terminal his-tag on dad1

   Depositor

   Stephen Harrison

   Article

   Miranda et al Nat Struct Mol Biol. 2005 Feb;12(2):138-

   Insert

   DASH / Dam1 complex (DAM1, ASK1, DUO1, SPC34, SPC19, DAD1, DAD2, DAD4, HSK3, DAD3 Budding Yeast)
   

   Use

   Tags

   his-tag on C-terminus of dad1

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21a-MKP7_K55R_153

    Plasmid

    #37794

    Depositor

    SeWon Suh

    Article

    Kim et al Proc Natl Acad Sci U S A. 2012 May 15;10

    Insert

    Mitogen-activated protein kinase phosphatase 7 (DUSP16 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    changed Lysnie 55 to Arginine and deleted amino a…

    Promoter

    Available since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pBrain-GFP-TACC3KDP∆678-681-shTACC3

    Plasmid

    #69115

    Purpose

    Dual-promoter plasmid to express knockdown-proof human TACC3 (amino acids 678-681 deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.

    Depositor

    Stephen Royle

    Article

    Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

    Inserts

    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    shTACC3
    

    Use

    RNAi

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of 678-681aa region. Silent mutations i…

    Promoter

    CMV

    Available since

    Oct. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. Str-KDEL_SBP-EGFP-CD59

    Plasmid

    #222307

    Purpose

    Synchronize the trafficking of CD59 from the ER.

    Depositor

    Franck Perez

    Article

    Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

    Insert

    Streptavidin-KDEL and CD59 fused to SBP-EGFP (CD59 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    signal peptide removed

    Promoter

    CMV

    Available since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5/FRT/TO GABARAP(5X/G116A)-mTagBFP2-GABARAP(5X)

    Plasmid

    #218426

    Purpose

    Expression of GABARAP split tandem protein with mTagBFP2, 5X: K2A, K13A, R15A, K20A, K23A

    Depositor

    Dieter Willbold

    Article

    Üffing et al Autophagy Reports, 3(1). doi: 10.1080/27

    Insert

    GABARAP(G116A/5X)-mTagBFP2-GABARAP(5X) (GABARAP Entacmaea quadricolor, Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    5X: K2A, K13A, R15A, K20A, K23A mutations of both…

    Promoter

    CMV

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2-GABARAP(5X)

    Plasmid

    #218425

    Purpose

    Expression of GABARAP split tandem protein with mTagBFP2, 5X: K2A, K13A, R15A, K20A, K23A

    Depositor

    Dieter Willbold

    Article

    Üffing et al Autophagy Reports, 3(1). doi: 10.1080/27

    Insert

    GABARAP(G116A)-mTagBFP2-GABARAP(5X) (GABARAP Entacmaea quadricolor, Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    5X: K2A, K13A, R15A, K20A, K23A mutations of down…

    Promoter

    CMV

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5/FRT/TO GABARAP(5X/G116A)-mTagBFP2-GABARAP

    Plasmid

    #212107

    Purpose

    Expression of GABARAP split tandem protein with mTagBFP2, 5X: K2A, K13A, R15A, K20A, K23A

    Depositor

    Dieter Willbold

    Article

    Üffing et al Autophagy Reports, 3(1). doi: 10.1080/27

    Insert

    GABARAP(5X/G116A)-mTagBFP2-GABARAP (GABARAP Entacmaea quadricolor, Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    5X: K2A, K13A, R15A, K20A, K23A mutations of upst…

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. K365-iLid

    Plasmid

    #188456

    Purpose

    Component of optically-induced motor dimerization system

    Depositor

    Zvonimir Dogic

    Article

    Lemma et al PNAS Nexus. 2023 Apr 12;2(5):pgad130. do

    Insert

    Kinesin 1-365 (Khc Fly)
    

    Use

    Tags

    6xHis and iLid

    Expression

    Bacterial

    Mutation

    Kinesin amino acids 1-365

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. K365-micro

    Plasmid

    #188457

    Purpose

    Component of optically-induced motor dimerization system

    Depositor

    Zvonimir Dogic

    Article

    Lemma et al PNAS Nexus. 2023 Apr 12;2(5):pgad130. do

    Insert

    Kinesin 1-365 (Khc Fly)
    

    Use

    Tags

    6xHis-MBP-TEV and SspB micro

    Expression

    Bacterial

    Mutation

    Kinesin amino acids 1-365

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEB1N-mApple-LZ-LOV2

    Plasmid

    #197418

    Purpose

    Mammalian expression of the mApple-tagged N-terminal half of photo-inactivated π-EB1

    Depositor

    Torsten Wittmann

    Article

    Dema et al Curr Biol. 2022 Mar 14;32(5):1197-1205.e

    Insert

    MAPRE1 (MAPRE1 Human)
    

    Use

    Tags

    A. sativa phototropin 1 LOV2 domain, GCN4 leucine…

    Expression

    Mammalian

    Mutation

    EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-lox-inv[Talpha1-Dre-pA]-lox-mOrange2-Cdk5rap2-CTD

    Plasmid

    #196881

    Purpose

    Neuron-specific expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mOrange2-Cdk5rap2-CTD
    

    Use

    Cre/Lox; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pB80-hsKIF5B(1-560)Rigor-L-2xHA-L-FRB

    Plasmid

    #193719

    Purpose

    Rapalog-inducible coupling to dimeric human kinesin-1 (KIF5B aa1-560) via FKBP for anterograde transport

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    hsKIF5B(1-560)Rigor-L-2xHA-L-FRB (KIF5B Synthetic, Human)
    

    Use

    Tags

    2xHA-FRB

    Expression

    Mammalian

    Mutation

    hsKIF5B(aa1-560), RIGOR: G234A

    Promoter

    Chicken beta-actin

    Available since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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