Skip to main content
Addgene
Showing: 1801 - 1820 of 1837 results
  1. MFE23-ZZ

    Plasmid
    #223553
    Purpose
    MFE23-ZZ, a bispecific adaptor molecule, was created by fusing a scFv, termed MFE23, recognizing carcinoembryonic antigen to protein ZZ, which binds to the Fc fragment of IgG.
    Depositor
    Insert
    pET302-MFE23-ZZ
    Use
    Tags
    HA tag and HIS tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
  2. pET22b Ag43 160N 6H sfGFP-R5 AmpR

    Plasmid
    #225139
    Purpose
    Recombinant Ag43 protein fused to silaffin-R5 and sfGFP. pelB as the periplasmic signal. His-tag for purification. TEV enzyme recognition site between Ag43 alpha subunit and the fluorescent protein.
    Depositor
    Insert
    Ag43 protein fused to silaffin-R5 and sfGFP
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
  3. 3xFN-Tel-TAL/pMXs-neo

    Plasmid
    #63591
    Purpose
    Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat. A retroviral expression vector with the neomycin resistance gene.
    Depositor
    Insert
    3xFVN-Tel-TAL
    Use
    Retroviral and TALEN
    Tags
    3xFLAG-tag, V5-tag, and nuclear localization sign…
    Expression
    Mammalian
    Mutation
    Promoter
    LTR
    Available since
    April 13, 2015
    Availability
    Academic Institutions and Nonprofits only
  4. pCDF-Az

    Plasmid
    #197101
    Purpose
    To express the azidophenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into azidophenylalanine
    Depositor
    Insert
    TyrRS variant, amber suppressor tRNA, kanamycin resistance gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Thr32, Asn107, Pro158, Leu159, Gln162, Arg286 (Ty…
    Promoter
    Available since
    June 15, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. pTYB11-PTBP1-RRM2CCtoSS

    Plasmid
    #89154
    Purpose
    recombinant expression of PTBP1-RRM2 C250S,C251S in e.coli
    Depositor
    Insert
    Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 2 mutant C250S, C251S (PTBP1 Human)
    Use
    Tags
    Intein and chitin binding domain
    Expression
    Bacterial
    Mutation
    mutations: Cys 250 to Ser, Cys 251 to Ser
    Promoter
    T7
    Available since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
  6. JWW-2 human chimeric monoclonal antibody

    Plasmid
    #66749
    Purpose
    Expresses a murine/human chimeric IgG1 HPV16 L2-specific neutralizing antibody that recognizes HPV16 L2 amino acid region 58-64 . JWW-2 works in ELISA/WB/HPV
    Depositor
    Inserts
    JWW-2 Heavy Chain
    JWW-2 Light Chain
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mEF1 and rEF1
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
  7. pUltraI-Tet3.0[TAA]

    Plasmid
    #164580
    Purpose
    Machinery plasmid containing a single copy of the Mb Tet3.0RS and cognate tRNA for incorporation of Tet3.0 at TAA codons
    Depositor
    Inserts
    Mb Tet3.0RS (R2-3)
    Mm tRNA[TAA]
    Use
    Tags
    Expression
    Bacterial
    Mutation
    L170G, N311G, C313A
    Promoter
    ProK and TacI
    Available since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. pSW031

    Plasmid
    #231144
    Purpose
    Used to express cyclic sortase cW11 (recognizes APXTG motif) from E. coli.
    Depositor
    Insert
    S. aureus Sortase A mutant
    Use
    Tags
    Nostoc punctiform split intein C-terminal fragmen…
    Expression
    Bacterial
    Mutation
    P94S, E108Q, S116V, D160N, T164Q, V168M, L169H, D…
    Promoter
    T7
    Available since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 1801 - 1820 of 1837 results