We narrowed to 8,680 results for: sgRNA

1. CROPseq-Puro-12xMS2

   Plasmid

   #212628

   Purpose

   MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

   Depositor

   Paul Blainey

   Article

   Najia et al bioRxiv 2024.10.01.616098

   Insert

   EF1a-PuroR-12xMS2-WPRE-hU6-sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. CROPseq-Puro-4xMS2

   Plasmid

   #212626

   Purpose

   MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

   Depositor

   Paul Blainey

   Article

   Najia et al bioRxiv 2024.10.01.616098

   Insert

   EF1a-PuroR-4xMS2-WPRE-hU6-sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9(BB)-2A-Puro-V2.0-sgHsAGO3_AH

   Plasmid

   #148855

   Purpose

   Mammalian Expression of HsAGO3-sgRNA

   Depositor

   Elisa Izaurralde

   Insert

   HsAGO3-sgRNA (AGO3 S.pyogenes)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. HCP1

   Plasmid

   #166103

   Purpose

   This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination.

   Depositor

   Matthias Heinemann

   Insert

   Msn2-sgRNA#27 (MSN2 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEM-2t

   Plasmid

   #159752

   Purpose

   CRISPR/Cas9 genome editing in plants; a template for PCR-derived fragments used in the assembly of polycistronic transcripts, where sgRNAs are separated by an Arabidopsis alanine tRNA.

   Depositor

   Wolfgang Lukowitz

   Article

   Angulo et al bioRxiv

   Insert

   sgRNA
   

   Use

   CRISPR

   Available Since

   Oct. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pCRISPRi_Mxi1_yl_NHEJ

   Plasmid

   #91249

   Purpose

   CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

   Inserts

   Codon optimized dCas9-Mxi1
   KU70 sgRNA expression cassette
   KU80a sgRNA expression cassette
   KU80b sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Promoter

   SCR1'-tRNA and UAS1B8-TEF(136)

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX-EGFP-g1

   Plasmid

   #107273

   Purpose

   eGFP sgRNA-1 and Cas9 expression vector (aka. pX-ps1)

   Depositor

   Knut Woltjen

   Article

   Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

   Insert

   GFP sgRNA-1
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSP2283

   Plasmid

   #70702

   Purpose

   Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSP2266

   Plasmid

   #70705

   Purpose

   Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRCA376 - pBA904 Puro-T2A-GFP NTC guide (pRCA360 backbone)

    Plasmid

    #238167

    Purpose

    Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    Non-targeting sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV.5’eGFP/sgLMNA

    Plasmid

    #170546

    Purpose

    Expresses a sgRNA for 5' tagging to LMNA and contains a cassette with eGFP without homology arms

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Insert

    sgRNA targeting 5'- end of LMNA
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZS160

    Plasmid

    #134238

    Purpose

    Lentiviral plasmid expressing sgRNA against HJURP

    Depositor

    Iain Cheeseman

    Article

    Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

    Insert

    Human HJURP sgRNA
    

    Use

    Lentiviral

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. GS2.1/EC

    Plasmid

    #132568

    Purpose

    sgRNA targeting A. thaliana pds3, regulated by A. thaliana U6 polIII promoter. Cas9 with a C-terminal mApple fusion, egg cell-specific expression. Hygromycin selectable marker (hpt).

    Depositor

    Mathias Pribil

    Article

    Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

    Insert

    egg cell promoter, Cas9-mApple, pds3 sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Promoter

    A. thaliana egg cell promoter

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJW1328

    Plasmid

    #69488

    Purpose

    sgRNA(F+E) targeting pha-1 (no Cas9); for co-conversion in C. elegans

    Depositor

    Jordan Ward

    Insert

    sgRNA(F+E) targeting pha-1
    

    Expression

    Worm

    Promoter

    R07E5.16 U6 promoter

    Available Since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQdCas9-sggfp

    Plasmid

    #236184

    Purpose

    The plasmid pQdCas9-sggfp expresses the dCas9 endonuclease and the sgRNA targeting the gfp gene.

    Depositor

    Radhakrishnan Mahadevan

    Insert

    Quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas9, and sgRNA for GFP gene
    

    Promoter

    Quorum sensing promoter

    Available Since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQdCas12a.sggfp(A)

    Plasmid

    #236038

    Purpose

    The plasmid pQdCas12a.sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene.

    Depositor

    Radhakrishnan Mahadevan

    Insert

    quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (A) of gfp gene
    

    Use

    CRISPR

    Promoter

    quorum sensing promoter

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSpCas9(BB)-2A-Puro-V2.0-sgHsAGO1_AH

    Plasmid

    #148854

    Purpose

    Mammalian Expression of HsAGO1-sgRNA

    Depositor

    Elisa Izaurralde

    Insert

    HsAGO1-sgRNA (AGO1 S.pyogenes)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSpCas9(BB)-2A-Puro-V2.0-sgHsNot1_AH

    Plasmid

    #148856

    Purpose

    Mammalian Expression of HsNot1-sgRNA

    Depositor

    Elisa Izaurralde

    Insert

    HsNot1-sgRNA (CNOT1 S.pyogenes)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDIV515

    Plasmid

    #177703

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    tRNA-sgRNA-tRNA
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Promoter

    RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. MSP2292

    Plasmid

    #70707

    Purpose

    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Mutation

    E782K, N968K and R1015H in SaCas9

    Promoter

    T7

    Available Since

    Dec. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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