We narrowed to 9,048 results for: sgRNA

Showing: 1801 - 1820 of 9048 results

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Mammalian_SaAID2S

Plasmid

#188652

Purpose

Expresses SaAID2S and gRNA in mammalian cells

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Inserts

AID2S-nSaCas9--UGI
Sa-sgRNA

Tags

UGI

Expression

Mammalian

Mutation

D10A for SaCas9

Promoter

EF-1a and H1

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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AAV_A2S-8

Plasmid

#188654

Purpose

AAV vector expressing SaAID-2S and gRNA

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Inserts

AID2S--nSaCas9-UGI
Sa-sgRNA

Use

AAV and CRISPR

Tags

UGI

Expression

Mammalian

Mutation

D10A for SaCas9

Promoter

Scp1 and U6

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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MTK0_001

Plasmid

#123924

Purpose

Encodes the spCas9 gRNA GFP dropout expression cassette with ConLS and ConRE connectors with Ampicillin resistance as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TU-sgRNA - L1/RE

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgATL3-5

Plasmid

#109011

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL3-sgRNA #5 (ATL3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

mU6 promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pX330-CENPA

Plasmid

#227282

Purpose

Expresses SpCas9 and a sgRNA targeting the N-terminus of CENPA for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of CENPA (CENPA Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pC130: pAAV.CMV-CasRx-VEGFA sgRNA

Plasmid

#203443

Purpose

Plasmid expressing active RfxCas13d with VEGFA mRNA targeting gRNA

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA
RfxCas13d

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG-sgRNA.FOXA-ZEB2_CRISPRd

Plasmid

#216170

Purpose

Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Insert

sgRNA- FOXA.bs- ZEB2.locus

Use

Lentiviral

Tags

EGFP

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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LRG-sgRNA.FOXA-ZEB2_CRISPRd_v2

Plasmid

#216173

Purpose

Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Insert

sgRNA- FOXA.bs- ZEB2.locus

Use

Lentiviral

Tags

EGFP

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-eSpCas9-2A-GFP-sgRNA-RDH11

Plasmid

#161924

Purpose

To generate RDH11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against RDH11 exon 2.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

sgRNA targeting RDH11 exon 2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pAK008

Plasmid

#128225

Purpose

classic sgRNA backbone for tRNA-gRNA array, Position 2

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

classic sgRNA backbone for tRNA-gRNA array, Position 2

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAK009

Plasmid

#128226

Purpose

classic sgRNA backbone for tRNA-gRNA array, Position 3

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

classic sgRNA backbone for tRNA-gRNA array, Position 3

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ1373_mU6_sgRNA targeting e1

Plasmid

#190684

Purpose

sgRNA targeting enhancer 1 of MYC

Depositor

Stanley Qi

Article

Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

Insert

sgRNA targeting enhancer 1 of MYC

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

BFP and Puromycin

Expression

Mammalian

Promoter

mouse U6 promoter

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro-V2.0-sgHsAGO1_AH

Plasmid

#148854

Purpose

Mammalian Expression of HsAGO1-sgRNA

Depositor

Elisa Izaurralde

Insert

HsAGO1-sgRNA (AGO1 S.pyogenes)

Use

CRISPR

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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Cas9 ROSA sgRNA mCherry

Plasmid

#155280

Purpose

Lentiviral Cas9 expression, ROSA sgRNA expression, mCherry marker

Depositor

Martin Carroll

Insert

spCas9

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pSpdCas9-hudTET1CD-T2A-GFP (PX458)

Plasmid

#129026

Purpose

inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-EGFP and cloning backbone for sgRNA

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-hudTET1CD, SgRNA cloning site

Tags

HA-Tag, NLS and T2A-EGFP

Expression

Mammalian

Mutation

dCas9 (D10A;H840A), catalytic domain huTET1 inact…

Promoter

CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgHK2_14

Plasmid

#239257

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting HK2

Depositor

Jason Cantor

Article

Huggler et al Nat Metab. 2026 Jan 16. doi: 10.1038/s42

Insert

sgRNA_14 targeting HK2 (HK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA_FANCF-mcherry

Plasmid

#159786

Purpose

S. pyogenes sgRNA collocated with pegRNA targeting human FANCF gene

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of sgRNA targeting FANCF gene (FANCF Human)

Expression

Mammalian

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-sgRNAs-del-ZNF91-mcherry

Plasmid

#202822

Purpose

Lentiviral vector modified to express two sgRNAs that delete exon 1 within the ZNF91 gene with EF-1apha for Cas9 and mcherry expression

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

two sgRNAs that delete exon 1 within the ZNF91 gene

Use

Lentiviral

Expression

Mammalian

Promoter

U6 - two

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pspCas9-ABE-3'UTR-sgRNA-2xMS2

Plasmid

#132554

Purpose

Vector plasmid expressing ABEmax and sgRNA scaffold with MS2 replacing the Tetraloop and loop 2

Depositor

Baisong Lu

Article

Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

Inserts

ABEmax
sgRNA, with Tetraloop and loop 2 replaced by MS2 aptamer

Expression

Mammalian

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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MSP2283

Plasmid

#70702

Purpose

Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Inserts

mammalian codon-optimized Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)

Use

CRISPR

Tags

NLS-3xFLAG

Expression

Bacterial

Promoter

T7

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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