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pPRISM-gal4-VP16-gcry-mRFP

Plasmid

#117778

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

gal4/VP16

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-Stop-cmlc2-eGFP

Plasmid

#117776

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

3x3 Stop sequence

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-MAVS(IRSp53 pLxIS)

Plasmid

#221315

Purpose

Expression of MAVS chimera with IRSp53 pLxIS domain in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

MAVS chimera with IRSp53 pLxIS domain (Mavs Mouse)

Use

Retroviral

Tags

FLAG

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pPRISM-TagRFP-gcry-mRFP

Plasmid

#117766

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-Stop-gcry-TagBFP

Plasmid

#117771

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

3x3 Stop sequence

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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CRY2clust-mCherry-RANK-P2A-CIBNcaax

Plasmid

#208613

Purpose

Mammalian expression of CRY2clust-mCherry-RANK-P2A-CIBNcaax (Opto-RANKm)

Depositor

Tomohiro Ishii, Takao Nakata

Article

Takada et al Sci Rep. 2024 Jan 19;14(1):1749. doi: 10

Insert

CRY2clust-mCherry-RANK-P2A-CIBNcaax (Cry2 Mouse, Mustard Weed)

Tags

CAAX and mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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Clover-LaminA-C-18

Plasmid

#56370

Purpose

Localization: Nuclear Envelope, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

LaminA (LMNA Human)

Tags

Clover

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV hSyn mCh-IRES-BoNT/B(1-146)-iLID

Plasmid

#122982

Purpose

AAV plasmid with human synapsin promoter driving mCh and BoNT/B amino acids 1-146 fused to iLID(V416I), separated with an IRES element. Co-express with SSPB-BoNT(147-441, Y365A) for sPA-BoNT

Depositor

Matthew Kennedy

Article

Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)

Insert

mCh-IRES-BoNT/B(1-146)-iLID

Use

AAV

Tags

mCh

Expression

Mammalian

Mutation

iLID contains long lived V416I mutation

Promoter

human synapsin

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-Fuse-cmlc2-TagBFP

Plasmid

#117786

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

mRFP

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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Cry2 mCherry GRK3 ct in pcDNA3.1

Plasmid

#64213

Purpose

mCherry fused between Cry2 and GRK3 ct to study cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Inserts

Cry2 PHR (CRY2 Mustard Weed)
GRK3 ct (GRK3 Human)

Tags

mcherry

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only