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pTA2EGFP

Plasmid

#193756

Purpose

Expresses EGFP under the control of PLTetO-1 promoter, ColE1 origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

EGFP

Expression

Bacterial

Promoter

PLTetO-1

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pTC3RBSC1cI

Plasmid

#193773

Purpose

Expresses lambda CI under the control of PLTetO-1 promoter, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

Lambda CI

Expression

Bacterial

Promoter

PLTetO-1

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pX*C3E2-Crimson

Plasmid

#193754

Purpose

Expresses E2-Crimson under the control of PLUX* promoter, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

E2-Crimson

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLUX*

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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PRC3MKO2

Plasmid

#193757

Purpose

Expresses mKO2 under the control of PR promoter, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

mKO2

Expression

Bacterial

Promoter

PR

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLC3RBSC2cI

Plasmid

#193775

Purpose

Expresses lambda CI under the control of PLLacO-1 promoter, p15A origin of replication, Chloramphenicol selection

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

Lambda CI

Expression

Bacterial

Promoter

PLLacO-1

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pJA2LUXR(F)

Plasmid

#193755

Purpose

Expresses LuxR in forward direction under the control of BBa_J23102 constitutive promoter, ColE1 origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

LuxR

Expression

Bacterial

Promoter

BBa_J23102

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcloDF-amp

Plasmid

#184844

Purpose

Backbone that confers Ampicillin resistance, contains cloDF ori

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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His8OphMA-21mer.

Plasmid

#189670

Purpose

His8 tagged OphMA with TEV cleavable C-terminus to generate 21mer and 18merNL.

Depositor

Markus Künzler, James Naismith

Article

Emmanuel et al bioRxiv 2022.07.21.500988

Insert

His8OphMATEV18mer

Tags

His8 tagged

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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His8OphMADeltaC6-18mer.

Plasmid

#189671

Purpose

His8 tagged OphMADeltaC6 with TEV cleavable C-terminus to generate truncated 18merTr and 15mer peptides

Depositor

Markus Künzler, James Naismith

Article

Emmanuel et al bioRxiv 2022.07.21.500988

Insert

His8OphMA∆C6-TEV18mer

Tags

His8 tagged

Expression

Bacterial

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pMOD_C'4800EC

Plasmid

#176875

Purpose

Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMOD_D4800EC

Plasmid

#176876

Purpose

Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMOD_A4800EC

Plasmid

#176873

Purpose

Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_B4800EC

Plasmid

#176874

Purpose

Empty backbone for Golden Gate cloning into Cermak, et al., 2017 Toolkit

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP206-AAV-1.3CaMKII-MCS-pA.

Plasmid

#78319

Purpose

AAV vector backbone containing a 1.3CaMKIIa promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Type

Empty backbone

Use

AAV

Promoter

1.3CaMKIIa

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP210-AAV-0.4CaMKII P-MCS-pA

Plasmid

#63084

Purpose

AAV2 vector backbone containing a 0.4CaMKIIa promoter followed by a multiple cloning site(MCS) followed by a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Type

Empty backbone

Use

AAV

Promoter

0.4αCaMKII

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pBbE15c-RFP

Plasmid

#126168

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23100

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pBbS9c-RFP

Plasmid

#126184

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23113

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBbS20a-RFP

Plasmid

#126192

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

S23200

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBbE14k-RFP

Plasmid

#126153

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBbE19a-RFP

Plasmid

#126161

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23105

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBbE13c-RFP

Plasmid

#126158

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23102

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBbE12a-RFP

Plasmid

#126145

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23151

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBbA12a-RFP

Plasmid

#126085

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23151

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pGEL086

Plasmid

#137894

Purpose

iSTU-CRISPR/Cas9 with RcCat intron, RZ structure backbone

Depositor

Yong Zhang

Article

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pFE-sfGFP

Plasmid

#132998

Purpose

pFE carrying a sfGFP gene

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Insert

sfGFP

Tags

6xhis

Expression

Bacterial

Promoter

tetR

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Bth

Plasmid

#129686

Purpose

(Empty backbone) Gateway entry clone with sgRNA under ZmUbi promoter

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

Empty Backbone

Use

CRISPR

Expression

Plant

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGEL077

Plasmid

#137886

Purpose

iSTU-CRISPR/Cas9 with StIV2 intron, NU structure backbone

Depositor

Yong Zhang

Article

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEL079

Plasmid

#137888

Purpose

iSTU-CRISPR/Cas9 with StIV2 intron, RZ structure backbone

Depositor

Yong Zhang

Article

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEL078

Plasmid

#137887

Purpose

iSTU-CRISPR/Cas9 with StIV2 intron, tRNA structure backbone

Depositor

Yong Zhang

Article

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFH131

Plasmid

#136550

Purpose

crRNA backbone for FnCas12a/CmsI nucleases, combine with OsU3 promoter module (pFH39)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

crRNA backbone for FnCas12a/CmsI nucleases, combine with OsU3 promoter module (pFH39)

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge