-
Plasmid#211465PurposeLentivirus backbone expressing human TFAM fused with BioID2DepositorInsertTFAM-BioID2
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-C-Myc-DDK-IRES-Puro HNRNPR
Plasmid#200730PurposeLentiviral expression of Flag-Tagged human HNRNPRDepositorInsertHNRNPR (HNRNPR Human)
UseLentiviralTagsMyc-DDK from backbone plasmidExpressionMammalianMutationPromoterCMVAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-Dnmt3a2-Ires2-mCherry-SV40
Plasmid#186969PurposePiggyBac vector encoding mouse Dnmt3a2 with Ires2-mCherry fluorescent marker for expression in mammalian cells.DepositorInsertDnmt3a2 (Dnmt3a Synthetic, Mouse)
UsePiggybacTagsFlagExpressionMammalianMutationIsoform 2 (residues 220–908)PromoterCAGAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRDAX-016 FLAG UnaG[1] IRES UnaG[2]
Plasmid#130126PurposePPI (BiFC/TriFC) assay vectorDepositorInsertUnaG
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-tight-DDdCas9VPPH-T2A-GFP-IRES-Neo
Plasmid#102892PurposePiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
UseCRISPRTagsExpressionMammalianMutationD10A, H840APromoterTRE-tightAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-tight-DDdCas9VPH-T2A-GFP-IRES-Neo
Plasmid#102890PurposePiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
UseCRISPRTagsExpressionMammalianMutationD10A, H840APromoterTRE-tightAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIG-Flag-Sox9-IRES-nls-GFP
Plasmid#44281DepositorInsertFlag-Sox9 (SOX9 Chicken)
UseTagsFlagExpressionMammalianMutationPromoterCMV-IE enhancer and Chicken beta-actin promoterAvailable sinceAug. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
plVX-EF1a-FLAG-hMETTL3-IRES-ZsGreen
Plasmid#207123PurposeLentiviral vector to express human METTL3DepositorInserthMETTL3 (METTL3 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Cb5-IRES-puromycin-pLVx-EF1a
Plasmid#177434PurposeTo express mCherry fused to Cb5 endoplasmic reticulum (ER) targeting sequence. Lentiviral vector used to make cell lines expressing this ER landmark.DepositorInsertCb5 tail anchor (Cyb5r4 Rat)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
piggyBac_rtTA (4th_Gen)_Gateway_IRES_NGN2-2A-PURO_Synapsin-BirA. (pEHA1618)
Plasmid#209084PurposeNgn2 mediated cortical neuron induction, gateway destination, Synapsin promoter BirA ligase expressionDepositorInsertsUseTagsExpressionMammalianMutationhuman codon optimizedPromoterhSynAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-tight-DDdCas9VPP300-T2A-GFP-IRES-Neo
Plasmid#102891PurposePiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-P300-T2A-GFP-IRES-Neo
UseCRISPRTagsExpressionMammalianMutationD10A, H840APromoterTRE-tightAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-MCS-dOB-hPOT1_IRES-blast
Plasmid#166413Purposeexpress hPOT1 ΔOB in mammalian cellsDepositorInsertProtection Of Telomeres 1 (POT1 Human)
UseLentiviralTagsmycExpressionMammalianMutationΔOB deleted 125 a.aPromoteref1-aAvailable sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Direct-seq_lentiGuide-Puro-tRNA-Tail-8A8G
Plasmid#157984PurposeExpresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.DepositorInsertS. pyogenes sgRNA cassette (tRNA-Tail-8A8G)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-C-Myc-DDK-IRES-Puro LARP7
Plasmid#200725PurposeLentiviral expression of Flag-Tagged human LARP7DepositorInsertLARP7 (LARP7 Human)
UseLentiviralTagsMyc-DDK from backbone plasmidExpressionMammalianMutationPromoterCMVAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BRD9_BET-PGK-Puro-IRES-GFP
Plasmid#75120PurposeRetroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4DepositorInsertBRD9 (BRD9 Human)
UseRetroviralTagsExpressionMutationBromodomain replaced with the first bromodomain o…PromoterAvailable sinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST flag-CAD E1368A
Plasmid#188141PurposeExpresses C-terminal flag-tagged human CAD E1368A in mammalian cellsDepositorInsertCAD (CAD Human)
UseRetroviralTagsFlag tagExpressionMammalianMutationTCC -> AGT silent mutations at nt439-441 elimi…PromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCV-EZH2_EZH1SET-PGK-Puro-IRES-GFP
Plasmid#75126PurposeRetroviral expression plasmid encoding a human EZH2 SET-domain-swap mutant containing the EZH1 SET domainDepositorInsertKMT6 (EZH2 Human)
UseRetroviralTagsNoneExpressionMutationSET domain replaced with the SET domain of EZH1PromoterAvailable sinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-HA tagged-Mouse Mdm2 wt-IRES-GFP
Plasmid#180491PurposeRole of Mdm2 splicing variants in tumorigenesisDepositorInsertmouse Mdm2 full length (wt)
UseRetroviralTagsHAExpressionMammalianMutationPromoterMSCVAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BRD9_N216A-PGK-Puro-IRES-GFP
Plasmid#75116PurposeRetroviral expression plasmid encoding a human BRD9 mutant carrying the N216A single amino acid mutationDepositorInsertBRD9 (BRD9 Human)
UseRetroviralTagsExpressionMutationsingle amino acid mutation N216APromoterAvailable sinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-tmeGFP-IRES-nls-mRuby2
Plasmid#63060PurposeExpresses truncated monomeric eGFP and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backboneDepositorInsertnls-mRuby2
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only