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mIFP-MannII-N-10
Plasmid#56229PurposeLocalization: Mannosidase/Golgi, Excitation: 683, Emission: 704DepositorInsertMannII
UseTagsmIFPExpressionMammalianMutationaa 1-112 of MannIIPromoterCMVAvailable sinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMSP1D1T1
Plasmid#97327PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional T residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1T1
UseTags6xHis - TEVExpressionBacterialMutationAddition of T residue after T25PromoterT7Available sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_His-TEV-EGFP
Plasmid#223723PurposeExpression of recombinant protein for purificationDepositorInsertEGFP, not monomeric (has A207)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCT221: pR6K-Donor(ZooTsy)_H1:12_E1:135_E2:20_H2:12
Plasmid#200874PurposeZooTsy donor harboring KanR cargo.DepositorInsertZooTsy
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCT401: pHelper(AjTn6022)_QF-separated_TniQ-K361D
Plasmid#200861PurposeInducible expression of AjTn6022 proteins.DepositorInsertAjTn6022
UseTagsExpressionBacterialMutationTniQ-K361DPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCT400: pHelper(AjTn6022)_QF-separated_TniQ-K348D
Plasmid#200860PurposeInducible expression of AjTn6022 proteins.DepositorInsertAjTn6022
UseTagsExpressionBacterialMutationTniQ-K348DPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMSIP103: pTarget(CyCAST)
Plasmid#200849PurposeTarget plasmid containing CyPSP1 and attachement site for CyCAST.DepositorInsertsPAM for CyCAST + CyPSP1
tRNA-Leu
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMSIP270: pHelper(CyCAST)_Shuffled-spacer_ΔTnsD
Plasmid#200845PurposeInducible expression of CyCAST proteins (except TnsD) with control crRNA for CyPSP1.DepositorInsertCyCAST
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMSIP124: pDonor(CyCAST)_0.5kb-cargo
Plasmid#200848PurposeCyCAST donor harboring non-functional cargo.DepositorInsertCyCAST
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMSIP267: pHelper(CyCAST)_PSP1_ΔTniQ
Plasmid#200842PurposeInducible expression of CyCAST proteins (except TniQ) with crRNA targeting CyPSP1.DepositorInsertCyCAST
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMSIP269: pHelper(CyCAST)_PSP1_ΔTnsD
Plasmid#200844PurposeInducible expression of CyCAST proteins (except TnsD) with crRNA targeting CyPSP1.DepositorInsertCyCAST
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMSIP116: pHelper(CyCAST)_PSP1
Plasmid#200840PurposeInducible expression of CyCAST proteins with crRNA targeting CyPSP1.DepositorInsertCyCAST
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMSIP122: pHelper(CyCAST)_Shuffled-spacer
Plasmid#200841PurposeInducible expression of CyCAST proteins with control crRNA for CyPSP1.DepositorInsertCyCAST
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3_5UTR_Myc_FLuc
Plasmid#229502PurposeMyc 5' untranslated region firefly luciferase reporter.DepositorInsertMyc 5'UTR
UseTagsExpressionMammalianMutationPromoterSV40Available sinceFeb. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-Evectin-2
Plasmid#221754PurposeStable expression of Evectin-2 in mammalian cellsDepositorInsertPLEKHB2 (PLEKHB2 Human)
UseRetroviralTagsGFPExpressionMammalianMutationtandem PLEKHB2(PH domain) (amino acids 1-110)PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-PLCd1(PHD)
Plasmid#221753PurposeStable expression of GFP-PLCd1(PHD) in mammalian cellsDepositorInsertPLCd1 (PLCD1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationamino acids 1–175PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-2xFYVE
Plasmid#221750PurposeStable expression of GFP-2xFYVE in mammalian cellsDepositorInserttandem HRS FYVE domain
UseRetroviralTagsGFPExpressionMammalianMutationtwo FYVE domains (amino acids 147-223), linked by…PromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIB-WIPI2b-mRuby3
Plasmid#221749PurposeStable expression of WIPI2b-mRuby3 in mammalian cellsDepositorInsertWIPI2b (WIPI2 Human)
UseRetroviralTagsmRuby3ExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIB-mRuby3-STX17TM
Plasmid#221744PurposeStable expression of mRuby3-STX17TM in mammalian cellsDepositorInsertSTX17 (STX17 Human)
UseRetroviralTagsmRuby3ExpressionMammalianMutationdeleted amino acids 1-228PromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only
