159,428 results

1. pLenti PGK Hygro DEST (w530-1)

   Plasmid

   #19066

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Hygro

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV_Anc689-NG-nCas9

   Plasmid

   #163527

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   Anc689-NG-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

   Promoter

   Available since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. TFORF2236

   Plasmid

   #141982

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   GATA4 (GATA4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-VHL-pRc/CMV

   Plasmid

   #19999

   Depositor

   William Kaelin

   Article

   Iliopoulos et al Nat Med. 1995 Aug . 1(8):822-6.

   Insert

   von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   Available since

   April 6, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMZ7

   Plasmid

   #223180

   Purpose

   pORI28 derivative with lacZ and upp gene inserted into the backbone; for generating gene-specific knockouts.

   Depositor

   Paul Blainey

   Article

   Zhu et al bioRxiv [Preprint]. 2023 Dec 30:2023.12.

   Type

   Empty backbone

   Use

   Chromosomal integration

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. SPDK3876(TRV-RNA2-pPEBV-MCS)

   Plasmid

   #149275

   Purpose

   Modified Tobacco rattle virus RNA2 vector that could be used for expression of proteins or RNAs

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Insert

   Modified TRV RNA2 with PEBV promoter
   

   Use

   T-dna vector

   Tags

   Expression

   Mutation

   T2318C (DNA) in PEBV coat protein sequence

   Promoter

   Available since

   June 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. miReporter-CAG

   Plasmid

   #82478

   Purpose

   microRNA reporter relying on a bidirectional CAG-based promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

   Insert

   H2B-mCherry and H2B-Citrine. HygroR
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330a dCas9-LSD1

   Plasmid

   #92362

   Purpose

   Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to LSD1. For targeted enhancer demethylation in chicken embryos.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   dCas9-LSD1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNESx2-EGFP-C1-PABDx2-Spo20

   Plasmid

   #220082

   Purpose

   PA biosensor with tandem Spo20-PABDs and two NES domains to aid in nuclear export

   Depositor

   Gerry Hammond

   Article

   Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

   Insert

   Spo20 (SPO20 Budding Yeast)
   

   Use

   Tags

   X. leavis map2k1.L(32-44)-GGSG-X. leavis map2k1.L…

   Expression

   Mammalian

   Mutation

   amino acids 51-91, tandem dimer

   Promoter

   CMV

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZac2.1 gfaABC1D-Ezr-eGFP

    Plasmid

    #176856

    Purpose

    Expresses Ezrin fused with eGFP at the C-terminus in astrocytes

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    Ezr-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    gfaABC1D

    Available since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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