163,606 results

1. pX458_Ruby

   Plasmid

   #110164

   Purpose

   Cas9 from S. pyogenes with Ruby2, and cloning backbone for sgRNA

   Depositor

   Philip Hublitz

   Article

   Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and Ruby2

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. EIF2AK2 gRNA (BRDN0001146342)

   Plasmid

   #75637

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human EIF2AK2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   EIF2AK2 (EIF2AK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMOD_C'0000a

   Plasmid

   #127226

   Purpose

   Empty Backbone for golden gate cloning compatible with Cermak., et al Toolkit.

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMOD_D0000a

   Plasmid

   #127230

   Purpose

   Empty Backbone for golden gate cloning compatible with Cermak., et al Toolkit.

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pegRNA-HEK3_del1-5-NGG

   Plasmid

   #185478

   Purpose

   pegRNA plasmid in order to make a 5 basepair deletion at the HEK3 locus, uses an NGG-PAM site.

   Depositor

   Sabine Fuchs

   Article

   Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

   Insert

   HEK3_del1-5-NGG pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF0701

   Plasmid

   #143065

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PAXBP1 (PAXBP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB-EF1a-MegaGate-DD-Hygro

   Plasmid

   #175267

   Purpose

   MegaGate destination with C-terminal DD tag and hygromycin resistance

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Tags

   Destabilization Domain

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMVV104

   Plasmid

   #215310

   Purpose

   Expresses sfGFP under induction with the arabinose Marionette Cassette (AraCAM + araE + PBAD-sfGFP) with a BBR1 backbone.

   Depositor

   Brian Pfleger

   Article

   Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   PAraBAD

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PX459-APP-KO

   Plasmid

   #176487

   Purpose

   To knockout APP gene

   Depositor

   Wade Harper

   Article

   Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

   Insert

   gRNA sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIB170

    Plasmid

    #90193

    Purpose

    E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pveg promoter

    Depositor

    Indranil Biswas

    Article

    Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

    Type

    Empty backbone

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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