159,557 results

1. Fuw-dCas9-Dnmt3a-P2A-tagBFP

   Plasmid

   #84569

   Purpose

   Lentiviral construct to express dCas9 fused with Dnmt3a-P2A-tagBFP

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

   Insert

   dCas9-Dnmt3a (DNMT3A Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA Sec13 pRK5

   Plasmid

   #46332

   Depositor

   David Sabatini

   Article

   Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

   Insert

   Sec13 (SEC13 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPB-TetOn-FUS-CHOP-FusionRed-FRB-iLID-WPRE-SFFV-MBP-SspB-irFP-NLS-WPRE

   Plasmid

   #223287

   Purpose

   A complete OptoMBP cassette for dissolution of FUS-CHOP condensates.

   Depositor

   Jared Toettcher

   Article

   Brumbaugh-Reed et al bioRxiv 2024.01.16.575860

   Insert

   myc-FUS-CHOP-FusionRed-FRB-iLID, MBP-SspB-irFP-NLS
   

   Use

   Synthetic Biology; Piggybac transposase insertabl…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. miRFP670-TRF1

   Plasmid

   #227388

   Purpose

   Label telomere, no adhesion

   Depositor

   Clifford Brangwynne

   Article

   Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

   Insert

   Telomeric miRFP (TERF1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAJE3-E7

   Plasmid

   #214359

   Purpose

   Expresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteins

   Depositor

   Ryan Mehl

   Article

   Eddins et al Bioconjug Chem. 2023 Dec 20;34(12):2243-

   Inserts

   2nd generation Tet2 "E7" aminoacyl tRNA synthetase
   Lpp promoted M. jannaschii tRNA
   Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
   Orthogonal "D4II" ColE1 origin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   AmpR and lpp

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMA7CR_2.0

   Plasmid

   #73950

   Purpose

   pMA7CR_2.0 contains arabinose inducible λ/RED β-protein and dam, and aTc inducible CRISPR/Cas9 and recX

   Depositor

   Alex Nielsen

   Article

   Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

   Inserts

   cas9
   lambda beta
   dam
   recX
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pAra and pTet

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CAG-iGlucoSnFR-TS

   Plasmid

   #127952

   Purpose

   Expresses the iGlucoSnFR-TS biosensor for intracellular [glucose], with a fluorescence-lifetime readout, in mammalian cells

   Depositor

   Gary Yellen

   Article

   Diaz-Garcia et al J Neurosci Res. 2019 May 20. doi: 10.100

   Insert

   iGlucoSnFR-TS
   

   Use

   AAV

   Tags

   His6 and Xpress epitope

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV.CamKII.GCaMP6s.WPRE.SV40

   Plasmid

   #107790

   Purpose

   AAV expression of ultrasensitive protein calcium sensor from the CamKII promoter

   Depositor

   James M. Wilson

   Has Service

   AAV9

   Insert

   GCaMP6s
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CamKII

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMAZ-SK

   Plasmid

   #73962

   Purpose

   self-killing gRNA plasmid pMAZ-SK

   Depositor

   Alex Nielsen

   Article

   Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

   Insert

   gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mCherry-Parkin-IRES-zeo

    Plasmid

    #61727

    Purpose

    Expression of human Parkin fused to mCherry

    Depositor

    Stephen Tait, Douglas Green

    Article

    Tait et al Cell Rep. 2013 Nov 27;5(4):878-85. doi:

    Insert

    Parkin (PRKN Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only