We narrowed to 2,067 results for: ENDOG

1. pUC19/PLIN2-GFP

   Plasmid

   #202194

   Purpose

   knock-in donor vector of GFP to the C-terminus of endogenous human PLIN2

   Depositor

   James Olzmann

   Article

   Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

   Insert

   PLIN2 (PLIN2 Human)
   

   Use

   Cloning vector

   Tags

   GFP knock-in

   Promoter

   lac

   Available Since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Th-iCre

   Plasmid

   #159459

   Purpose

   Targeting vector to TH gene locus for induction of P2A-fused iCre, with PGK-puroR drug resistant cassette flanked by FRT sequence.

   Depositor

   Yuejun Chen

   Article

   Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

   Insert

   P2A-iCre
   

   Use

   Cre/Lox

   Tags

   P2A

   Expression

   Mammalian

   Promoter

   Endogenous TH

   Available Since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAM1

   Plasmid

   #51401

   Purpose

   Expresses truncated version of NLL-MetRS from endogenous MetG promoter

   Depositor

   David Tirrell

   Article

   Mahdavi et al Proc Natl Acad Sci U S A. 2014 Jan 7;111

   Insert

   MetRS (metG E coli)
   

   Mutation

   L13N, Y260L, H301L; Please note that a truncated …

   Promoter

   MetG

   Available Since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. IR25a-T2A-QF-AD

   Plasmid

   #162526

   Purpose

   Plasmid for CRISPR-mediated knock-in line that expresses the activation domain of QF by knocking-in a T2A-QF-AD fragment replacing the stop codon of the endogenous Aedes aegypti Ir25a gene

   Depositor

   Leslie Vosshall

   Article

   Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

   Insert

   Ir25a-left-HDR-arm; T2A-QF2AD-Zip+-SV40; 3xP3-EYFP-SV40; Ir25a-right-HDR-arm
   

   Expression

   Insect

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ORCO-T2A-QF-DBD

   Plasmid

   #162525

   Purpose

   Plasmid for CRISPR-mediated knock-in line that expresses the DNA-binding domain of QF by knocking-in a T2A-QF-DBD fragment replacing the stop codon of the endogenous Aedes aegypti Orco gene

   Depositor

   Leslie Vosshall

   Article

   Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

   Insert

   Orco-left-HDR-arm; T2A-Zip-::QFDBD-SV40; 3xP3-dsRed-SV40; Orco-right-HDR-arm
   

   Expression

   Insect

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSL1180-HR-Aag2-loxP-PUb-RFP-loxP-3xFLAG-AGO1

   Plasmid

   #162164

   Purpose

   Selectable homology donor template for Aag2 cell AGO1

   Depositor

   Charles Rice

   Article

   Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

   Inserts

   RFP
   3xFLAG
   

   Use

   CRISPR and Cre/Lox; Homology donor template

   Tags

   AGO1 homology and loxP

   Expression

   Insect

   Promoter

   Ae. aegypti PUb and endogenous AGO1

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG-sp-myc-mSema4D

   Plasmid

   #190645

   Purpose

   Expresses Mouse Sema4D with N-terminal myc after endogenous Signal peptide

   Depositor

   Andrew Newman, Victor Tarabykin

   Article

   Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

   Insert

   Sema4d (Sema4d Mouse)
   

   Tags

   myc

   Expression

   Mammalian

   Available Since

   July 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHiUGE TurboID-HA donor (all-in-one) ORF2

   Plasmid

   #200385

   Purpose

   Tagging endogenously expressed proteins with TurboID-HA, C-terminal

   Depositor

   Scott Soderling

   Article

   Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

   Insert

   TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)
   

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHiUGE TurboID-HA donor (all-in-one) ORF1

   Plasmid

   #200384

   Purpose

   Tagging endogenously expressed proteins with TurboID-HA, C-terminal

   Depositor

   Scott Soderling

   Article

   Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

   Insert

   TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)
   

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ppk301-T2A-QF2 HDR plasmid

    Plasmid

    #130667

    Purpose

    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti ppk301 gene

    Depositor

    Leslie Vosshall

    Article

    Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

    Insert

    ppk301-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; ppk301-right-HDR-arm
    

    Use

    Donor template

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBrain-GFP-FKBP-TACC3-shTACC3

    Plasmid

    #59354

    Purpose

    Knocks down endogenous TACC3 and expresses RNAi-resistant human TACC3 tagged with GFP & FKBP in mammalian cells.

    Depositor

    Stephen Royle

    Article

    Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

    Insert

    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    

    Use

    RNAi

    Tags

    EGFP and FKBP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBrain-GFP-TACC3KDP-shTACC3

    Plasmid

    #59356

    Purpose

    Knocks down endogenous TACC3 and re-expresses knockdown-proof (RNAi-resistant) GFP-tagged human TACC3.

    Depositor

    Stephen Royle

    Article

    Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

    Insert

    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    

    Use

    RNAi

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Silent mutations in amino acids 30-33 to confer s…

    Promoter

    CMV

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHS0766 pET45b(+) His14-MBP-TEV-AloTnpB-2 3' extension

    Plasmid

    #176542

    Purpose

    Bacterial expression of His14-MBP-TEV-AloTnpB-2 with 3' extension derived from locus

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Inserts

    MBP tag
    TnpB
    

    Tags

    3' extension derived from endogenous locus a…

    Expression

    Bacterial

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p.UTA.2.0 miR-27a-3p

    Plasmid

    #82438

    Purpose

    Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3p

    Depositor

    Jens Gruber

    Article

    Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

    Insert

    hsa-miR-27a-3p (MIR27A Human)
    

    Mutation

    Three copies hsa-miR-27a-3p Complementary region …

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CIB-VP16 (B1014)

    Plasmid

    #92036

    Purpose

    Expresses CIB1 (full length, with endogenous NLS) fused to VP16 activation domain.

    Depositor

    Chandra Tucker

    Article

    Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

    Insert

    CIB1 (CIB1 Mustard Weed)
    

    Tags

    Fusion of plant CIB1 (intact NLS) with VP16 activ…

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pIS1-Vim/Actb5UTR-renilla

    Plasmid

    #38238

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    Vim promoter with Actb 5UTR/Renilla (Vim, Actb Mouse)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Actb promoter (1000 nt upstream) and 5' UTR …

    Promoter

    Endogenous Vim

    Available Since

    Nov. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

17. RepairTemplate_Smc4-mAID-HaloTAG

    Plasmid

    #217658

    Purpose

    Repair template to endogenously tag SMC4 with mAID-Halo Tag

    Depositor

    Daniel Gerlich

    Article

    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

    Insert

    SMC4-mAID-HaloTag (SMC4 Human)
    

    Expression

    Bacterial and Mammalian

    Available Since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSPCas9(BB)-2A-GFP_ABCA3GFP_targeting

    Plasmid

    #188541

    Purpose

    Plasmid encoding pCas9 and gRNA targeting the endogenous human ABCA3 locus stop codon

    Depositor

    Darrell Kotton

    Article

    Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    GFP

    Promoter

    U6

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSLQ8666 pHR-RRM2p-BFP-T2A-N term (1-406) dLbCpf1-EE12RR345L-EFS-Zeo-WPRE

    Plasmid

    #140228

    Purpose

    RRM2p-driven expression of BFP and N terminal dCpf1 (Cas12a) 406-split half with leucine zipper. Constitutive EFS-driven zeocin resistance.

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    dLbCpf1 (Cas12a) N terminal half (406) with zipper
    

    Use

    Lentiviral

    Tags

    BFP

    Expression

    Mammalian

    Promoter

    RRM2 endogenous promoter

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pONSY-CoNMM:mCherry

    Plasmid

    #111878

    Purpose

    This plasmid expresses mCherry fluorescent protein fused to an N-Myristoylation motif (NMM) from the endogenous Src2 gene (CAOG_06360). It can be used to visualize the membrane and filopodia.

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Insert

    Capsaspora N-Myristoylation motif (CoNMM) from the Src2 gene fused to mCherry
    

    Use

    Capsaspora owczarzaki

    Tags

    mCherry

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge