We narrowed to 5,538 results for: pcas

1. pCAGGS puro HA-hUSP27X G76S

   Plasmid

   #225719

   Purpose

   Transfection of USP27X (G76S) with HA tag in mammalian cells

   Depositor

   Francisco Bustos

   Article

   Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

   Insert

   USP27X G76S (USP27X Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   G76S

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCambia-dLB-kanR-GFP

   Plasmid

   #223177

   Purpose

   Plant Transformation Vector that allows marker removal through Mendelian inheritance. Start of DNA transfer at RB, terminates at left border but in ~1/3 of cases goes ahead until second left border

   Depositor

   Andreas Stuermer

   Type

   Empty backbone

   Use

   Ti plasmid for agrobacterium

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 26, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pJS-BCD-Strep-PCaspase

   Plasmid

   #214034

   Purpose

   Bacterial expression plasmid for strep-tagged PCaspase (Prokaryotic Caspase) from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   PCaspase
   

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJS-BCD-Strep-dPCaspase

   Plasmid

   #214035

   Purpose

   Bacterial expression plasmid for strep-tagged catalytically dead PCaspase (Prokaryotic Caspase) from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   PCaspase
   

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   H79A, C146A

   Promoter

   T7 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-nEF-SpCas9D10A nickase

   Plasmid

   #216736

   Purpose

   Expresses SpCas9-D10A nickase from a nEF promoter. For AAV packaging. Derived from pAAV-nEF-SpCas9 (Addgene #87115)

   Depositor

   Vincent Dion

   Article

   Alvaro et al bioRxiv

   Insert

   Cas9-D10A nickase
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   EF-1-alpha core

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCR8-PspCas13b_gRNA[ccdbCam]-2xPP7

   Plasmid

   #196846

   Purpose

   backbone for gRNA cloning of dpspCas13b tagged by 2xPP7

   Depositor

   Albert Cheng

   Article

   Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCR8-PspCas13b_gRNA[ccdbCam]_1xMS2b

   Plasmid

   #196847

   Purpose

   backbone for gRNA cloning of dpspCas13b tagged by 1xMS2

   Depositor

   Albert Cheng

   Article

   Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. gzk328-dpspCas13b_gRNA[ccdbCam]-10xPBSc_Loop

   Plasmid

   #196844

   Purpose

   backbone for gRNA cloning of dpspCas13b tagged by 10xPBSc_Loop

   Depositor

   Albert Cheng

   Article

   Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. gzk326-dpspCas13b_gRNA[ccdbCam]-5xPBSc_Loop

   Plasmid

   #196845

   Purpose

   backbone for gRNA cloning of dpspCas13b tagged by 5xPBSc_Loop

   Depositor

   Albert Cheng

   Article

   Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS-AnkG ZU5in-3xHA

    Plasmid

    #213730

    Purpose

    Mammalian cell expression vector for overexpression of AnkG ZU5in isoform in motor neurons.

    Depositor

    Chaolin Zhang

    Article

    Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

    Insert

    AnkG ZU5in (Ank3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-SpCas9HF1-P2A-puro

    Plasmid

    #214357

    Purpose

    Expresses SpCas9HF1 and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SpCas9HF1 and puromycin resistance genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N497A/R661A/Q695A/Q926A

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS-AnkB ZU5ex-3xHA

    Plasmid

    #213732

    Purpose

    Mammalian cell expression vector for overexpression of AnkB ZU5ex isoform in motor neurons.

    Depositor

    Chaolin Zhang

    Article

    Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

    Insert

    AnkB ZU5ex (Ank2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS-AnkG ZU5ex-3xHA

    Plasmid

    #213731

    Purpose

    Mammalian cell expression vector for overexpression of AnkG ZU5ex isoform in motor neurons.

    Depositor

    Chaolin Zhang

    Article

    Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

    Insert

    AnkG ZU5ex (Ank3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCaUra3-CUbo(K48R)-yeGFP

    Plasmid

    #212772

    Purpose

    C-terminal PCR-based tagging with CUbo(K48R)-yeGFP in budding yeast with CaUra3 selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCaUra3-CUbo(K63R)-yeGFP

    Plasmid

    #212773

    Purpose

    C-terminal PCR-based tagging with CUbo(K63R)-yeGFP in budding yeast with CaUra3 selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEJS2194-RP_PiggyBac_SpCas9ABE8e-tracrRNA-Puro

    Plasmid

    #211821

    Purpose

    PiggyBac vector expressing SpCas9-ABE8e with tracrRNA for stable cell line establishment

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

    Insert

    SpCas9-ABE8e, tracrRNA and Puromycin resistance gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    chicken β-actin promoter, U6 promoter and hPGK pr…

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGIG-FLAG-SHTN1LRRR>GGG

    Plasmid

    #209570

    Purpose

    Mammalian expression of Shtn1 long isoform with RG mutation

    Depositor

    Sika Zheng

    Article

    Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

    Insert

    Shtn1 long isoform with RG mutation (4930506M07Rik Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    RRR to GGG

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Cav2.2 BBS exon37a pCAGGS

    Plasmid

    #206086

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II and alternative exon 37a

    Depositor

    Annette Dolphin

    Article

    Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/B-actin

    Available since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Cav2.2 HA EIVA (E1658A) pCAGGS

    Plasmid

    #206082

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain IV which reduces trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E1658A

    Promoter

    CMV/B-actin

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Cav2.2 HA EIA (E314A) pCAGGS

    Plasmid

    #206081

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain I which reduces trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E314A

    Promoter

    CMV/B-actin

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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