We narrowed to 32,430 results for: uros

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pLVX-Puro-TDP-43-D169G

Plasmid

#133757

Purpose

Lentiviral expression of human TDP-43 D169G disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed aspartate 169 to glycine.

Promoter

CMV

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pIRES Centrin1 Cherry Puro

Plasmid

#64340

Purpose

Bicistronic expression of Centrin-mCherry fusion and Puro resistance gene

Depositor

Matthieu Piel

Insert

Centrin1

Tags

IRES-Puro and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only
pLenti CMV Puro dn-cHSF1

Plasmid

#134728

Purpose

Lentiviral expression vector for constitutively active dominant-negative HSF1

Depositor

Matthew D. Shoulders

Article

Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

Insert

dn-cHSF1 (HSF1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Deletion of amino acids 186-202, 379−529

Promoter

CMV

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP1.v1 puro

Plasmid

#87790

Purpose

Expresses an inducible short hairpin targeting human MKP1 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP1 (DUSP1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE tight

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-4493NES(Booster-PKA)

Plasmid

#184710

Purpose

PKA biosensor

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

Booster-PKA

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-Smad4

Plasmid

#89829

Purpose

Knockdown human Smad4 expression in human mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

Insert

Smad4 shRNA (SMAD4 Mouse, Human)

Use

Retroviral

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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Anti-Neuroligin-1 [N97A/31R]

Plasmid

#114554

Purpose

Mammalian Expression Plasmid of anti-Neuroligin-1 (Rat). Derived from hybridoma N97A/31.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Neuroligin-1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Nlgn1 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-PARG shRNA

Plasmid

#23260

Depositor

Robert Sobol

Article

Tang et al Mol Cancer Res. 2010 Jan;8(1):67-79. doi

Insert

poly(ADP-ribose) glycohydrolase shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

March 31, 2010

Availability

Academic Institutions and Nonprofits only
pSBtet-2xMARS-nGFP-puro

Plasmid

#205239

Purpose

DOX inducible expression of two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells from the Sleeping Beauty transposon system

Depositor

Jeremy Baskin

Article

Cao et al Nat Cell Biol. 2024 Oct;26(10):1804-1816

Insert

2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)

Use

Transposon

Tags

HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

Expression

Mammalian

Mutation

amino acids 143-271 of PLEKHA5 (GenBank reference…

Promoter

tight TRE promoter

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRSITEP-puro-shWRN1-C911

Plasmid

#125784

Purpose

a seed-matched control for pRSITEP-puro-shWRN1

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

shWRN1-C911 (WRN Human)

Use

RNAi

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-TET2Puro-gal4DBD-miniVPR-HA

Plasmid

#207169

Purpose

Dox Inducible Expression Plasmid for gal4DBD-miniVPR-HA

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Insert

Gal4DBD-miniVPR

Use

Lentiviral and Synthetic Biology

Tags

HA

Expression

Mammalian

Promoter

TRE3G

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Pou5f1-g2)-PGKpuroBFP-W

Plasmid

#105036

Purpose

Lentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Pou5f1 (Pou5f1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Teton-puro-shOCT4

Plasmid

#198761

Purpose

conditional knockdown of OCT4

Depositor

Hyonchol Jang

Article

Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

Insert

shOCT4 (POU5F1 Human)

Expression

Mammalian

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-IFITM3g1 (BB15)

Plasmid

#139460

Purpose

Lentiviral vector with gRNA targeting IFITM3; includes puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

IFITM3-targeting sgRNA inserted; resistance gene: puroR (IFITM3 Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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FUGW-APOE-E4-T2A-Puro

Plasmid

#190810

Purpose

Express APOE E4 allele from Ubiquitin C promoter, co-expressing Puro resistance gene

Depositor

Ronald Hart

Insert

APOE (APOE Human)

Use

Lentiviral

Mutation

E4

Promoter

Ubi C

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-mTurquoise2-Puro

Plasmid

#169224

Purpose

Targeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Puro at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mTurquoise2-P2A-Puro

Use

Targeting vector backbone

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-CTRLg1 (CG509)

Plasmid

#139455

Purpose

Lentiviral vector with non-targeting gRNA and puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

Non-targeting sgRNA inserted; resistance gene: puroR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Tsc2-g1)-PGKpuroBFP-W

Plasmid

#105039

Purpose

Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Tsc2 (Tsc2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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TET-pLKO.1 PURO shID2 #2

Plasmid

#83087

Purpose

Lentiviral shRNA vector for inducible knockdown of human ID2

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shID2 (ID2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pMKO.1-Puro-shPP2A-Abeta

Plasmid

#15250

Depositor

William Hahn

Article

Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

Insert

PP2A regulatory subunit A, beta isoform (PPP2R1B Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only