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pIG-830_HA-GD2-28z_CAR_tNGFR_Retroviral

Plasmid

#207507

Purpose

This plasmid can be used to generate retrovirus.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

tNGFR, HA-GD2-28z_CAR (NGFR Human)

Use

Retroviral

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pEFIRES-P-ACSL3-mCherry

Plasmid

#87158

Purpose

Fluorescent protein targeted to lipid droplets (LDs) from the endoplasmic reticulum (ER)

Depositor

Elina Ikonen

Article

Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

Insert

ACSL3 (ACSL3 Human)

Tags

mCherry

Expression

Mammalian

Promoter

EF-1-alpha

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-Opti

Plasmid

#163126

Purpose

LentiCRISPRv2 variant with modified sgRNA scaffold from Chen et al. 2013 Cell.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pJD849

Plasmid

#245490

Purpose

Binary Plasmid for plant transformation expressing RUBY from the 35S promoter and the terminator from the Arabidopsis thaliana HSP gene. Kanamycin plant selection

Depositor

Beth Rowan

Insert

p35S-RUBY

Expression

Plant

Promoter

35S

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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M51

Plasmid

#242620

Purpose

Strep-MYC-His protein expression in bacteria

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

Strep-MYC-His (MYC Human)

Tags

His and Strep

Expression

Bacterial

Available Since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_EML4:1-981

Plasmid

#211241

Purpose

Mammalian expression of full-length human EML4. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

EML4:M1-S981 (EML4 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

I382V natural variant (VAR_031727)

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-FL-syt7

Plasmid

#213630

Purpose

Bacterial expression of SUMO-tagged mouse FL-syt7

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

FL-syt7 (Syt7 Mouse)

Tags

SUMO

Expression

Bacterial

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pClneoMyc rat S-SCAM alpha

Plasmid

#40213

Depositor

Yutaka Hata, Yoshimi Takai

Article

Hirao et al J Biol Chem. 1998 Aug 14;273(33):21105-1

Insert

S-SCAM (Magi2 Rat)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
pET 28 full-length human PARP1

Plasmid

#192635

Purpose

Expresses human PARP1 with an N-terminal His-tag

Depositor

John Pascal

Article

Langelier et al J Biol Chem. 2008 Feb 15;283(7):4105-14.

Insert

Human PARP1 (PARP1 Human)

Tags

histidine-tag

Expression

Bacterial

Mutation

Polymorphism A762

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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L4440_BioBrick-SCR-sgRNA-A

Plasmid

#177811

Purpose

Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

Scrambled sgRNA targeting sequences

Expression

Bacterial

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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