We narrowed to 2,342 results for: clu
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Plasmid#140202PurposeCRISPRa proof-of-concept test target with fluorescent reporter. PelcA is fused to mCherry, with low basal expression in Aspergillus nidulans. Fungal vector with AMA1 and pyrG selection marker.DepositorInsertmCherry
UseCRISPR and Synthetic Biology; Proof-of-concept te…TagsExpressionMutationG174DPromoterPelcAAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-mir451 EF1Alpha-puro-T2A-BFP
Plasmid#164792PurposeExpress miR-451 under the U6 promoter in mammalian cellsDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterEF1Alpha and U6Available sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRI018-pKW20088-PelcA-mCherry-TtrpC-PU3-E1-Cas9Scaffold-8xT
Plasmid#140205PurposeProof-of-concept of CRISPR/dSpCas9-VPR activation, encoding PelcA-mCherry along with the sgRNA E1 expression cassette, targeting PelcA.DepositorInsertsmCherry
sgRNA E1
UseCRISPR and Synthetic Biology; Proof-of-concept te…TagsExpressionMutationG174DPromoterPelcA and U3Available sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
p6349 MSCV-CMV-Flag-HA-Brd4 1224-1362
Plasmid#31355DepositorInsertBrd4 1224-1362 (BRD4 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCRI008-PgpdA-Cas12aScaffold-BsmbI-Cas12aScaffold-TrcpT
Plasmid#140201PurposeFungal vector for one-step cloning of LbCas12a crRNA arrays and expression. Pgpda and pyroA are BsmbI domesticated.DepositorTypeEmpty backboneUseCRISPR and Synthetic Biology; Cloning of crrna an…TagsdLbCas12a crRNA scaffoldExpressionMutationPromoterPgpdAAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
eGFP-KIF5A-Rigor
Plasmid#172202PurposeExpresses KIF5A G235A rigor mutant tagged with eGFP in mammalian cellsDepositorInsertKIF5A (Kif5a Mouse)
UseTagseGFPExpressionMammalianMutationChanged Glycine 235 to AlaninePromoterCMVAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmirGLO-Bmpr2-Δ17
Plasmid#49380PurposeInsert is a fragment of the Bmpr2 3'-UTR that contains a mutated miR-17 binding site. To be used for 3'UTR assay.DepositorInsertBmpr2 (BMPR2 Mouse, Human)
UseLuciferaseTagsExpressionMutationchanged nucleotides AC to TG at bp 7347-7348PromoterHuman phosphoglycerate kinase (PGK) promoterAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSOMA-T679A-NES
Plasmid#118940PurposeUsed to express the T679A mutant form of the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thalianaDepositorInsertSOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Exclusion Signal
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
His10-B1 Integrin
Plasmid#186139PurposeFor expression of recombinant his10-beta1 integrin cytosolic domain in bacteriaDepositorInsertITGB1 (ITGB1 Human)
UseTagsMBP + uncleavable His10ExpressionBacterialMutationPromoterLacAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRLCon1-3180
Plasmid#31445DepositorInsertHNF4A 3'UTR (long) (HNF4A Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only