We narrowed to 13,730 results for: TIM

1. pEF1a-hMLH1NTD-NLS

   Plasmid

   #174826

   Purpose

   Mammalian expression of human MLH1NTD-NLS (codon optimized)

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   human MLH1_NTD-NLS (codon optimized)
   

   Use

   Tags

   SV40 bpNLS

   Expression

   Mammalian

   Mutation

   truncation of residues 1-335

   Promoter

   EF1a

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSW002-Pc-mNeonGreen

   Plasmid

   #205016

   Purpose

   Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mNeonGreen (codon optimized for P. fluorescens)

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mNeonGreen is codon optimized for expression in P…

   Promoter

   Pc

   Available since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAG-FLEX-oG-WPRE-SV40pA

   Plasmid

   #74292

   Purpose

   pAAV vector to express oG (optimized Glycoprotein) in a Cre-dependent manner

   Depositor

   Edward Callaway

   Article

   Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

   Insert

   oG (optimized Glycoprotein)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   chimeric glycoprotein

   Promoter

   CAG

   Available since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-ABEmax-NRCH

   Plasmid

   #136923

   Purpose

   Expresses ABEmax-NRCH in mammalian cells

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   ABEmax-NRCH
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   see manuscript

   Promoter

   CMV

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST40-MCU-V5-HIS

   Plasmid

   #31731

   Depositor

   Vamsi Mootha

   Article

   Baughman et al Nature. 2011 Jun 19. ():.

   Insert

   MCU (MCU Human)
   

   Use

   Tags

   V5-HIS6X

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMTL3

   Plasmid

   #127966

   Purpose

   constitutive expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus

   Depositor

   Martin Kampmann

   Article

   Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

   Insert

   AAVS1-CAG-KRAB-dCas9-BFP-KRAB
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3 p53 S15A

   Plasmid

   #69004

   Purpose

   mammalian expression of human p53 S15A mutant.

   Depositor

   David Meek

   Article

   Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S15A

   Promoter

   CMV

   Available since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMTL5

   Plasmid

   #127967

   Purpose

   DHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locus

   Depositor

   Martin Kampmann

   Article

   Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

   Insert

   AAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST47-MCU-GFP

   Plasmid

   #31732

   Depositor

   Vamsi Mootha

   Article

   Baughman et al Nature. 2011 Jun 19. ():.

   Insert

   MCU (MCU Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   *

   Promoter

   CMV

   Available since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Twitch-2B pRSETB

    Plasmid

    #48203

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Insert

    Twitch-2B
    

    Use

    Tags

    6xHIS, cpVenusCD, and mCerulean3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CaMKIIa-Flpo

    Plasmid

    #183535

    Purpose

    INTRSECT

    Depositor

    Karl Deisseroth

    Article

    Fan et al Cell. 2023 Feb 2;186(3):543-559.e19. doi

    Insert

    Flpo
    

    Use

    AAV

    Tags

    Expression

    Mutation

    None

    Promoter

    CaMKIIa

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV2_CAG_oROS-G

    Plasmid

    #216115

    Purpose

    Encodes the genetically encoded, green fluorescent peroxide sensor oROS-G in AAV viral vectors.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.01.31.578117

    Insert

    oROS-G
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-T7-AcrIIA16_Efa-NLS(SV40) (KAC459)

    Plasmid

    #134337

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Efa with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIA16_Efa
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-T7-hAcrVA1-NLS(sv40) (BPK5050)

    Plasmid

    #115136

    Purpose

    CMV-T7 promoter expression plasmid for human codon optimized AcrVA1 anti-CRISPR protein with C-terminal NLS

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy, Keith Joung

    Article

    Marino et al Science. 2018 Sep 6. pii: science.aau517

    Insert

    human codon optimized AcrVA1 anti-CRISPR protein
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAB1679 CMV-HIVNES-GS-Cas13bt3

    Plasmid

    #176317

    Purpose

    CMV-HIVNES-GS-Cas13bt3 (human codon optimized Cas13bt3 expression)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Insert

    human codon optimized Cas13bt3
    

    Use

    CRISPR

    Tags

    HIV NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCW-tTRE-scFv-SunTag

    Plasmid

    #193138

    Purpose

    Expresses scFv intracellular antibody component of the SunTag system under Dox regulation

    Depositor

    Brad Rosenberg

    Article

    Danziger et al PLoS Pathog. 2022 Apr 14;18(4):e1010464.

    Insert

    scFv-SunTag
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZR158_Lenti-U6-gRNA-10xCS1-PP7_hPGK-PCP-P65-HSF1-Puro

    Plasmid

    #180273

    Purpose

    Lentiviral expression vector for CRISPRa-SAM sgRNA with 10x capture sequence, and PCP-P65-HSF1 cassette

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDNA5/FRT/TO_TOMM20_HaloTag9

    Plasmid

    #169335

    Purpose

    Expression of HaloTag9 at the mitochondrial surface of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    TOMM20-HaloTag9 (TOMM20 Human)
    

    Use

    Tags

    HaloTag9

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR-PGK- dCas9-SunTag-P2A-HygR

    Plasmid

    #193137

    Purpose

    Expresses dCas9 fused to the SunTag scaffold

    Depositor

    Brad Rosenberg

    Article

    Danziger et al PLoS Pathog. 2022 Apr 14;18(4):e1010464.

    Insert

    dCas9-SunTag-T2A-HygR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HaloTag-3xrGBD

    Plasmid

    #176108

    Purpose

    Visualization of active Rho

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    rhotekin (Rtkn Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMVdel

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Twitch-2B pcDNA3

    Plasmid

    #49531

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Insert

    Twitch-2B
    

    Use

    Tags

    ECFP and cpCit174

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p415 TEF roGFP2‐Tsa2ΔCPΔCR

    Plasmid

    #83239

    Purpose

    The catalytically inactive genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCPΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.

    Depositor

    Tobias Dick

    Article

    Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do

    Insert

    roGFP2-Tsa2dCpdCr
    

    Use

    Tags

    roGFP2 fusion with Tsa2dCpdCr

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pZR040_Lenti-U6-sgCtrl-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

    Plasmid

    #180266

    Purpose

    Lentiviral expression vector for CRISPRa-SAM with non-targeting control sgRNA

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    No-Target Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Str-KDEL_TNF-SBP-RpHLuorin2

    Plasmid

    #171722

    Purpose

    Synchronize trafficking of TNFa-RpHLuorin2

    Depositor

    Geert van den Bogaart

    Article

    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

    Insert

    TNF (TNF Human)
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and ratiometri…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV-T7-AcrIIA16_Lmo-NLS(SV40) (KAC508)

    Plasmid

    #134332

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Lmo with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIA16_Lmo
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-BE4max-NRRH

    Plasmid

    #136918

    Purpose

    Expresses BE4max-NRRH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    BE4max-NRRH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV-T7-AcrIIC1-NLS(SV40) (KAC208)

    Plasmid

    #134340

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIC1 with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIC1
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-BE4max-NRCH

    Plasmid

    #136920

    Purpose

    Expresses BE4max-NRCH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    BE4max-NRCH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. 6691 Bicistronic_ires_puro

    Plasmid

    #64335

    Purpose

    This a retroviral expression plasmid with a minimal polylinker followed by IRES and puro resistance gene

    Depositor

    Georgios Stathopoulos

    Article

    Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV-BE4max-NRTH

    Plasmid

    #136919

    Purpose

    Expresses BE4max-NRTH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    BE4max-NRTH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3.1(+)_Pmel-1_TCRB

    Plasmid

    #175288

    Purpose

    Expresses Pmel-1 TCR beta chain

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    T cell receptor beta chain (Tcrb Mouse)
    

    Use

    Suitable for ivt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.1(+)_Pmel-1_TCRA

    Plasmid

    #175287

    Purpose

    Expresses Pmel-1 TCRA alpha chain

    Depositor

    Jeff Hammerbacher

    Article

    Gottschalk et al bioRxiv 2021.11.30.470597

    Insert

    T cell receptor alpha chain (Tcra Mouse)
    

    Use

    Suitable for ivt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDIV301

    Plasmid

    #204969

    Purpose

    AMA1 plasmid with Aspergillus optimized Mad7 and ble (bleomycin) resistance marker

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

    Inserts

    Mad7
    ble (bleomycin resistance marker)
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans tef1 promoter and Aspergillu…

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. C1A-C1A-EYFP

    Plasmid

    #61155

    Purpose

    Diacylglycerol (DAG) reporter

    Depositor

    Tobias Meyer

    Article

    Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

    Insert

    C1A-C1A-EYFP, C1A from PKCG (aa 26-89, rat) (Prkcg Rat)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    C1A from PKCG amino acids 26-89

    Promoter

    CMV

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. XE120 CAMKII-K42M 1-271-CS2+

    Plasmid

    #16734

    Depositor

    Randall Moon

    Article

    Kühl et al J Biol Chem. 2000 Apr 28. 275(17):12701-

    Insert

    CAMKII-K42M 1-271 (Camk2a Rat)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    C-terminal truncation of K42M kinase

    Promoter

    Available since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

36. pMY-IRES-eGFP

    Plasmid

    #163361

    Purpose

    Retroviral backbone for in-frame cloning of a gene of interest and an IRES separated GFP marker

    Depositor

    Teunis Geijtenbeek, Louis Ates

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMY-MCS-P2A-CD90.2

    Plasmid

    #163355

    Purpose

    Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated CD90.2 surface marker

    Depositor

    Teunis Geijtenbeek, Louis Ates

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMY-MCS-P2A-eGFP

    Plasmid

    #163356

    Purpose

    Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated GFP marker

    Depositor

    Teunis Geijtenbeek, Louis Ates

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDule2-Acd82

    Plasmid

    #174100

    Purpose

    Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.

    Depositor

    Ryan Mehl

    Article

    Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.

    Insert

    Acridone 82 tRNA synthetase and tRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N311S, C313G, V366A, W382T

    Promoter

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. EGFP gRNA (BRDN0000561167)

    Plasmid

    #80034

    Purpose

    3rd generation lentiviral gRNA plasmid targeting EGFP

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge