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Addgene

We narrowed to 6,909 results for: CAD

Showing: 4651 - 4700 of 6909 results
  1. MZB160

    Plasmid
    #217841
    Purpose
    Bacterial expression of 10xHis-SUMO (Smt3) fusion to HRV-3C cleavable Halotagged Arf (L8K+Q71L) for pulldown assays
    Depositor
    Insert
    Arf1 (ARF1 Human)
    Use
    Tags
    10x His, HaloTag, and SUMO (SMT3)
    Expression
    Bacterial
    Mutation
    L8K, Q71L
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
  2. MZB222

    Plasmid
    #221772
    Purpose
    Bacterial expression of 10xHis-SUMO (Smt3) fusion to Sec7 domain (687-885) of Big1 (ArfGEF1) for rapid nucleotide exchange and activation of Arf GTPases
    Depositor
    Insert
    ArfGEF1 (ARFGEF1 Human)
    Use
    Tags
    10xHis-SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  3. MZB224

    Plasmid
    #226998
    Purpose
    Bacterial expression of 10xHis-SUMO (Smt3) fusion to HRV-3C cleavable Halotagged Arf (T31N) for pulldown assays
    Depositor
    Insert
    Arf1 (ARF1 Human)
    Use
    Tags
    10x His, HaloTag, and SUMO (SMT3)
    Expression
    Bacterial
    Mutation
    T31N
    Promoter
    T7
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. pJL NarL YdfI Flag

    Plasmid
    #225650
    Purpose
    Expresses NarL with a mutated DNA binding domain (YdfI). For use with NarX chimeras and reporter plasmids from this study.
    Depositor
    Insert
    NarL-YdfI (narL Synthetic)
    Use
    Cell free expression
    Tags
    FLAG
    Expression
    Bacterial
    Mutation
    NarL with native DNA-binding domain replaced with…
    Promoter
    T7
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pTE5418_pMBP-GlcR

    Plasmid
    #221192
    Purpose
    Expression vector for 10x-MBP-GlcR (MGlcR). GlcR is a glycolate-responsive transcriptional repressor and the gene product of pden4400 from Paracoccus denitrificans, codon optimized for E. coli.
    Depositor
    Insert
    glcR (pden4400 from Paracoccus denitrificans)
    Use
    Tags
    10x His tag with E. coli maltose binding protein …
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter and T7 promoter-lacO
    Available since
    Sept. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pTE5419_p3WJdB-glcO36

    Plasmid
    #221193
    Purpose
    GlcR module template with glcO36 operator: T7 promoDNA template of GlcR sensor with glcO36 operator: T7 promoter-glcO36-3WJdB reporter-T7 terminator linear DNA tter-glcO36-3WJdB reporter-T7 terminator
    Depositor
    Insert
    T7 promoter-glcO36
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter with downstream glcO36 operator
    Available since
    Sept. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. pDY279

    Plasmid
    #182959
    Purpose
    Str resistant,CoE1* ori. CRISPR/Cas9 gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations in ptsI. (for 1st round editing)
    Depositor
    Insert
    gRNA (ttcaggcattttagcatccc), homologous repair template for ptsI
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 4651 - 4700 of 6909 results