We narrowed to 3,332 results for: car-t

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pLz76_CB_operon_CsoS2_N4

Plasmid

#140858

Purpose

Same as pLz37 but with CsoS2 truncated such that NTD repeats N1, N2, and N3 are removed

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CbbL, CbbS, CsoS2 (delN1-N3), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D

Expression

Bacterial

Mutation

deleted amino acids 1-189 of CsoS2

Available Since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

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pBz109_CsoS2_NTD

Plasmid

#140842

Purpose

His-tagged CsoS2 N-terminal domain (NTD), pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 N-terminal domain (NTD)

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

deleted amino acids 262-869

Promoter

T7

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLz47_polyPro_CsoS2_Npeptide

Plasmid

#140845

Purpose

Consensus N*-peptide after polyproline II helix, N-terminal His- tag used as BLI bait, pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

polyproline helix with CsoS2 N-peptide

Tags

Hexahistidine tag and Strep-tag II

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBz110_CsoS2_CTD

Plasmid

#140844

Purpose

His-tagged CsoS2 C-terminal domain (CTD), pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 C-terminal domain (CTD)

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

deleted amino acids 1-594

Promoter

T7

Available Since

May 8, 2020

Availability

Academic Institutions and Nonprofits only

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tetO-NR2F1

Plasmid

#170698

Purpose

doxycycline-inducible overexpression of NR2F1

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

nuclear receptor subfamily 2 group F member 1 (NR2F1 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Mutation

deletion of 36G- please see depositor comments

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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tetO-HAND2

Plasmid

#170690

Purpose

doxycycline-inducible overexpression of HAND2

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

HAND2 (HAND2 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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psicheck2-B4GALT3mut

Plasmid

#203602

Purpose

Reporter plasmid carrying B4GALT3 3'UTR with mutated miR27b target sequences.

Depositor

Hiroyuki Mizuguchi

Article

Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

Insert

beta-1,4-galactosyltransferase 3 (B4GALT3 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Seed sequences in miR-27b target sequences were m…

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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psicheck2-MAIP1mut

Plasmid

#203604

Purpose

Reporter plasmid carrying MAIP1 3'UTR with mutated miR27b target sequences

Depositor

Hiroyuki Mizuguchi

Article

Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

Insert

matrix AAA peptidase interacting protein 1 (MAIP1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

miR-27b target sequences in the 3'UTR was mu…

Promoter

SV40

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLAG-Claspin

Plasmid

#12659

Depositor

Michele Pagano

Article

Peschiaroli et al Mol Cell. 2006 Aug 4. 23(3):319-29.

Insert

Claspin (CLSPN Human)

Tags

Flag

Expression

Mammalian

Available Since

May 16, 2007

Availability

Academic Institutions and Nonprofits only
pWR58

Plasmid

#202795

Purpose

integrating bioluminescene reporter for Cas9 activity in S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

P-TDH3/coCBG(first portion)-YUM1
P-TEF1/ShBle/T-ACT1
YUM1-coCBG(last portion)/T-ADH2
URA3 and flanking sequence

Expression

Yeast

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pTIM

Plasmid

#81198

Purpose

pTIM in vitro transcription backbone plasmid with two multiple cloning sites and two Rho-independent transcriptional terminators.

Depositor

Andrés Vázquez-Torres

Article

Tapscott et al Sci Rep. 2018 Jun 21;8(1):9465. doi: 10.

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 1, 2016

Availability

Academic Institutions and Nonprofits only

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2333_Cas9-CtIP-dnRNF168

Plasmid

#200254

Purpose

This plasmid expresses the Cas9-CtIP-dnRNF168 in mammalian cells

Depositor

Claudio Mussolino

Article

Carusillo et al Nucleic Acids Res. 2023 May 22;51(9):466

Insert

Cas9-CtIP-dnRNF168

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR-BS-AtMIR390a-B/c

Plasmid

#199559

Purpose

GATEWAY-compatible entry vector

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acids Res. 2023 Oct 27;51(19):10

Insert

Basal stem of Arabidopsis MIR390a precursor including a chloramphenicol-ccdB cassette flanked by two inverted BsaI sites (MIR390a Mustard Weed)

Use

Gateway-compatible entry vector

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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XRE-pNL1.3[secNLuc]

Plasmid

#182295

Purpose

Secreted Luciferase reporter for AhR activity

Depositor

Severine Degrelle, Thierry Fournier

Article

Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

Insert

Xenobiotic Response Element (CYP1A1 Human)

Tags

secNluc

Expression

Mammalian

Promoter

XRE

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pET30b jGCaMP8f F366H

Plasmid

#204139

Purpose

Production of recombinant calcium sensor jGCaMP8f F366H high dynamic range variant in E.coli

Depositor

Katalin Torok

Article

Tran et al Front Cell Neurosci. 2023 May 18;17:1155

Insert

jGCaMP8f F366H

Tags

N-terminal His tag

Expression

Bacterial

Mutation

Phenylalanine 366 changed to histidine

Promoter

T7

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pET30b jGCaMP8f L27A

Plasmid

#204138

Purpose

Production of recombinant calcium sensor jGCaMP8f L27A fast decay variant in E.coli

Depositor

Katalin Torok

Article

Tran et al Front Cell Neurosci. 2023 May 18;17:1155

Insert

jGCaMP8f L27A

Tags

N-terminal His tag

Expression

Bacterial

Mutation

Leucine 27 changed to Alanine

Promoter

T7

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMAL-XPG

Plasmid

#39215

Depositor

Aziz Sancar

Article

Matsunaga et al J Biol Chem. 1995 Sep 1;270(35):20862-9.

Insert

XPG (ERCC5 Human)

Tags

MBP

Expression

Bacterial

Mutation

Note that XPG starts at amino acid 5

Promoter

Ptac

Available Since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-Necl-5-ScNeo

Plasmid

#170283

Purpose

To monitor the status of Necl-5, the plasmid encodes a recombinant Necl-5 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Ichise et al Elife. 2022 Feb 3;11. pii: 76269. doi: 1

Insert

a recombinant mouse Necl-5 fused to the mScarlet and to the mNeonGreen fluorescent protein (Pvr Synthetic, Mouse)

Tags

mScarlet and mNeonGreen

Expression

Mammalian

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMeCP2Y pc644

Plasmid

#167565

Purpose

The complete rat MeCp2 ORF is fused in frame of the NH2-terminus of the enhanced YFP (pEYFP-N1 vector; CLONTECH Laboratories, Inc.)

Depositor

Cristina Cardoso

Article

Brero et al J Cell Biol. 2005 Jun 6;169(5):733-43. d

Insert

Methyl-CpG binding protein 2 (Mecp2 Rat)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCKC775

Plasmid

#226626

Purpose

piggyBAC 7xTCF-LEF hCas9-T2A-A/T-biased2

Depositor

Chang Liu

Article

Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

Inserts

7xTCF-LEF, SpCas9, T2A, A/T-biased2 variant of TdT (DNTT Human, S. pyogenes)
EF1a promoter, sfGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

7xTCF-LEF and EF1a

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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