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EF1a-DIO-PSAML-141F,Y115F- GlyR-2A-FusionRed

Plasmid

#226590

Purpose

Pharmacologically Selective Actuator Module, chimeric ligand-gated ion channels that can be used to manipulate electrophysiological activity.

Depositor

D. James Surmeier

Insert

PSAML-141F,Y115F-GlyR

Use

AAV

Tags

P2A-Fusion Red

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pX552-EF1a-DIO Chronos-eGFP(with gRNA scaffold)

Plasmid

#199582

Purpose

Expresses Chronos-eGFP in a Cre-dependent manner

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

Chronos eGFP

Use

AAV, CRISPR, and Cre/Lox

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1a intron A

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC1642 - pAAV EF1a EGFP-KASH with mTACR1 gRNAs

Plasmid

#195018

Purpose

An adeno-associated viral vector expressing eGFP-KASH and two guides targeting mouse TACR1

Depositor

Christopher Richie

Article

Belilos et al bioRxiv [Preprint]. 2023 Oct 9:2023.02.0

Inserts

CCGTATAGGCGGCTGCCCAA
EGFP-KASH
TTCCGTGGTGGGCAACGTAG

Use

AAV

Tags

KASH domain of Nesprin2

Expression

Mutation

Promoter

EF1a, hU6, and mU6

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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p2708-pHAGE2-Ef1aL-dsRed-UBC-tagBFP-loxP

Plasmid

#216477

Purpose

lentiviral dual expression of dsRed and tagBFP with loxP site for Cre excision

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Inserts

dsRed
tagBFP

Use

Cre/Lox and Lentiviral

Tags

Expression

Mutation

Promoter

EF1aL and UBC

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1(delDBD)-EF1a-TagRFP-2A-tet3G

Plasmid

#204214

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (delDBD) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Deletion of DNA binding domain (delDBD)

Promoter

TetON

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1-R162del-EF1a-TagRFP-2A-tet3G

Plasmid

#204191

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (R162del) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Deletion of R162

Promoter

TetON

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1-I207M -EF1a-TagRFP-2A-tet3G

Plasmid

#204213

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (I207M) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Point mutation on I207M

Promoter

TetON

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1-Q175∗-EF1a-TagRFP-2A-tet3G

Plasmid

#204211

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (Q175∗) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Point mutation on Q175* (*termination)

Promoter

TetON

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1-R178∗-EF1a-TagRFP-2A-tet3G

Plasmid

#204210

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (R178∗) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Point mutation on R178* (*termination)

Promoter

TetON

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NKX2-1-I207F -EF1a-TagRFP-2A-tet3G

Plasmid

#204212

Purpose

Doxycycline inducible lentiviral vector for human NKX2-1 (I207F) overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NK2 homeobox 1 (NKX2-1 Human)

Use

Lentiviral

Tags

Point mutation on I207F

Expression

Mammalian

Mutation

Promoter

TetON

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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vYM143_phiC31-nTRE-pEF1s(low)(CG793)-H2b-Citrine

Plasmid

#197686

Purpose

"pEF1s(low)" reporter plasmid, with CpG793 mutated to CG

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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vYM142_phiC31-nTRE-pEF1s(high)(CC793)-H2B-citrine

Plasmid

#197685

Purpose

"pEF1s(high)" reporter plasmid, with CpG793 mutated to CC

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-GFP ArgSen delta Degradation Domain

Plasmid

#184692

Purpose

Lentiviral transfer plasmid for expression of GFP ArgSen delta Degradation Domain

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

GFP ArgSen delta Degradation Domain

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-GFP ArgSen delta Disordered Region

Plasmid

#184693

Purpose

Lentiviral transfer plasmid for expression of GFP ArgSen delta Disordered Region

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

GFP ArgSen delta Disordered Region

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-GFP ArgSen delta Rad23b UbL

Plasmid

#184694

Purpose

Lentiviral transfer plasmid for expression of GFP ArgSen delta Rad23b UbL

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

GFP ArgSen delta Rad23b UbL

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-GFP ArgSen delta Degradation Domain

Plasmid

#184701

Purpose

Lentiviral transfer plasmid for expression of GFP ArgSen delta Degradation Domain

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

GFP ArgSen delta Degradation Domain

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-GFP ArgSen delta Disordered Region

Plasmid

#184702

Purpose

Lentiviral transfer plasmid for expression of GFP ArgSen delta Disordered Region

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

GFP ArgSen delta Disordered Region

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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EK0312 pAAV-EF1a-DIO-EYFP-WPRE-HGHpA

Plasmid

#191148

Purpose

AAV expression of a Cre reporter with EYFP output; derived from a gift from prof. Liqun Luo

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

DIO-EYFP

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

hEF1a

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-nCoV2019-N-YBBR-2xStrep-IRES-Puro

Plasmid

#185450

Purpose

Expresses SARS-CoV-2 nucleocapsid protein with a c-terminal YBBR and Strep tag in mammalian cells

Depositor

Ahmet Yildiz

Article

Jack et al PLoS Biol. 2021 Oct 11;19(10):e3001425.

Insert

Nucleocapsid (N SARS-CoV-2)

Use

Lentiviral

Tags

Strep-Tag II and YBBR

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-XL1/BRCT1-Linker-eGFP

Plasmid

#176084

Purpose

EGFP fused to the C-terminus of a XL1/BRCT1 domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

XL1/BRCT1 (XRCC1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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