161,727 results

1. pLX313-Firefly luciferase

   Plasmid

   #118017

   Purpose

   Constitutive expression of Firefly luciferase

   Depositor

   William Hahn, David Root

   Article

   Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-CMV-Galpha-oA ONE-GO

   Plasmid

   #189732

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Inserts

   Galpha-oA ONE-GO
   GNAO (Gnao1 Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   I250 (silent mutation in YFP)

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pRSFDuet-1_DNAseI

   Plasmid

   #182408

   Purpose

   Bacterial expression of DNaseI

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   DNase I (DNASE1 Bovine)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-Ftractin-mCherry

   Plasmid

   #85131

   Purpose

   lentiviral expression of an F-actin live-cell marker (mCherry)

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Insert

   Ftractin-mCherry (amino acids 9-52 of rat ITPKA, C-temrinally tagged with mCherry)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCVD442

   Plasmid

   #11074

   Depositor

   James Kaper, Michael Donnenberg

   Article

   Donnenberg et al Infect Immun. 1991 Dec . 59(12):4310-7.

   Insert

   sacB (sacB Bacillus subtilis)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV-mito-Citron1

   Plasmid

   #134305

   Purpose

   mitochondria-targeted direct-response citrate sensor

   Depositor

   Robert Campbell

   Article

   Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

   Insert

   Citron1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-FLEX-TeLC-P2A-dTomato

   Plasmid

   #159102

   Purpose

   Cre-dependent tetanus toxin light chain construct with nuclear-localized dTomato fluorophore for conditional silencing of neurons.

   Depositor

   Sandeep Datta

   Article

   Pashkovski et al Nature. 2020 Jul;583(7815):253-258. doi:

   Insert

   TeLC-P2A-NLS-dTomato
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSynapsin

   Available since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-C-dTAG-Puro

   Plasmid

   #207727

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. rtTA-N144

   Plasmid

   #66810

   Purpose

   2nd generation lentiviral transfer plasmid. Expresses the reverse tetracycline-controlled transactivator (rtTA) with hygromycin resistance

   Depositor

   Andrew Yoo

   Article

   Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi

   Insert

   Reverse tetracycline-controlled transactivator
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human EF1a

   Available since

   Sept. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSPgRNA

    Plasmid

    #47108

    Purpose

    Expresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Has Service

    Cloning Grade DNA

    Insert

    SPgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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