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Plasmid#170705Purposedoxycycline-inducible overexpression of TBX20DepositorInsertTBX20 (TBX20 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pGEX6P1-mCherry-anti-mouse-IgG(kappa)-nanobody
Plasmid#138130PurposeExpresses GST-tagged mCherry-anti-mouse-IgG(kappa)-nanobody in E. coli cells.DepositorInsertmCherry-anti-mouse-IgG(kappa)-nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.GCaMP6f.WPRE.SV40
Plasmid#100835PurposeAAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6f
UseAAVTagsExpressionMammalianMutationGCaMP3-T302L R303P A317E D380Y T381R S383T R392GPromoterCAGAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDule2-Acd82
Plasmid#174100PurposeAcridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.DepositorInsertAcridone 82 tRNA synthetase and tRNA
UseTagsExpressionBacterialMutationN311S, C313G, V366A, W382TPromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME-3xNLS-mTagBFP2-Cre (JDW 918)
Plasmid#224517PurposeA Gateway-compatible middle entry clone containing a 3xNLS-mTagBFP2-Cre fusionDepositorInsert3xNLS-mTagBFP2-Cre
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET23b-2E2-HA-mEGFP-6xHis
Plasmid#129594PurposeThe encoded protein is the anti-HA frankenbody variant fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.DepositorInsertAnti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
UseTags6xHis-tagExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGIPz-PD-L1 WT
Plasmid#121486Purpose2nd generation lentiviral vector for over expressing human PD-L1 WT with PD-L1 shRNADepositorInsertPD-L1
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Voltron
Plasmid#119033PurposeExpress Voltron in mammalian cellsDepositorInsertVoltron
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Synapsin sHRPa-NRX in FSW lentiviral vector
Plasmid#73147PurposeLarge fragment of split HRP fused to the extracellular terminus of neurexin3B. This is a lentiviral plasmid, but can also be used for transfections. Synapsin promoter.DepositorInsertsHRPa-NRX
UseLentiviralTags2x HA tag and Flag tagExpressionMammalianMutationPromoterSynapsinAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-Ace-mNeon2
Plasmid#195526PurposeGreen fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoterDepositorInsertAce-mNeon2
UseAAVTagsExpressionMammalianMutationAce-mNeon 81S; SY linkerPromoterCaMKIIAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNCS-CyOFP
Plasmid#74278PurposeConstitutive expression of CyOFP in bacteriaDepositorInsertCyOFP
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-EGFP-NLS
Plasmid#86677PurposeExpresses nuclear-localized GFP after lentiviral transduction. Can be used to monitor GFP leakage into the cytosol following nuclear envelope rupture events.DepositorInsertEGFP-NLS
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mCherry-Cenexin
Plasmid#186435PurposeExpresses mCherry Cenexin in mammalian cells and zebrafish embryosDepositorInsertCenexin (ODF2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
Daxx/pEGFP
Plasmid#119021PurposeExpress GFP-Daxx in mamalian cellsDepositorInsertdeath-domain associated protein (Daxx) (DAXX Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP-CREB
Plasmid#137001PurposemEGFP-CREB donor for G-CREBDepositorInsertCREB1 (Creb1 Rat)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-2E2-HA-mEGFP
Plasmid#129595PurposeThe encoded protein is the anti-HA frankenbody variant fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.DepositorInsertAnti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-Ataxin3Q28
Plasmid#22122DepositorInsertAtaxin 3 (ATXN3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-JEDI-1P-WPRE
Plasmid#202611PurposeDouble floxed genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector expressed under the mammalian promoter (EF1a)DepositorInsertJEDI-1P
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-H2B-6
Plasmid#58101PurposeLocalization: Nucleus/Histones, Excitation: 554, Emission: 581DepositorInsertH2B (H2BC11 Human)
UseTagstdTomatoExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceDec. 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-FLEX-RiboL1-jGCaMP8f
Plasmid#192618PurposeAAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8f.DepositorInsertFLEX-RiboL1-jGCaMP8f
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-ATR-C
Plasmid#53769Purposemammalian expression of flag-tagged ATR C-terminal fragmentDepositorInsertATR (ATR Human)
UseTagsFlagExpressionMammalianMutationC-terminal domain, aa 1398-2644*PromoterCMVAvailable sinceJuly 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1661-sgMUC4-E3(F+E)
Plasmid#51025PurposeLentiviral vector that contains an optimized S. pyogenes sgRNA targeting the repetitive sequence of human MUC4 exon 3DepositorInsertoptimized sgRNA (MUC4 Human, Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6Available sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_gRNA1TERTKO_BsagRNA5tertko_2A-GFP
Plasmid#198863PurposePlasmid encoding for 2 gRNAs targeting the human TERT geneDepositorInsertTERT sgRNA (TERT Human)
UseTagsExpressionMammalianMutationPromoteru6Available sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag4/hL3MBTL1
Plasmid#28231DepositorInsertl(3)mbt-like 1 (Drosophila) (L3MBTL1 Human)
UseTagsFLAGExpressionMammalianMutationIrrelevant S49T mutationPromoterAvailable sinceFeb. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pmax pona 12xTRICK 24xMS2SL
Plasmid#64542PurposeTRICK translation biosensor with 12x copies of modified PP7 stem loops and 24x MS2 stem loops.DepositorInsert12x PP7 stem-loops
UseTags24xMS2 stem loops, HA, NLS, and dNP799ExpressionMammalianMutationPromoterponAAvailable sinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFP5370 ss-FIREmate-HA- KDEL-IVS-IRES- TNF-SBP- mCherry-FIREtag
Plasmid#216696PurposeSynchronize trafficking of TNFalpha from the ER (RUCH system)DepositorInsertsFIREmate-KDEL
Tumor Necrosis Factor alpha (TNFa)
UseTagsFIREtag, Streptavidin Binding Protein (SBP), and …ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-ATR-N
Plasmid#53767Purposemammalian expression of flag-tagged ATR N-terminal fragmentDepositorInsertATR (ATR Human)
UseTagsFlagExpressionMammalianMutationN-terminal domain, aa 1-1409PromoterCMVAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
Twitch-2B pcDNA3
Plasmid#49531PurposeFluorescent reporter for calcium signalingDepositorInsertTwitch-2B
UseTagsECFP and cpCit174ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-ATR-M
Plasmid#53768Purposemammalian expression of flag-tagged ATR middle regionDepositorInsertATR (ATR Human)
UseTagsFlagExpressionMammalianMutationmiddle domain, aa 593-1700*PromoterCMVAvailable sinceJuly 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-Flag-H2A K118-119R
Plasmid#63564PurposeExpression of human H2A with K118R and K119R tagged with FlagDepositorInsertH2A (H2AC20 Human)
UseTagsFlag (MDYKDDDDKVPRIQ)ExpressionMammalianMutationLysine 118 and Lysine 119 are now ArgininePromoterCMVAvailable sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV-mCherry-KIX-mCherry
Plasmid#137004PurposemCherry-KIX acceptor for G-CREBDepositorInsertKIX domain (Crebbp Rat)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pPEP-TEV cNup98 (1-498)
Plasmid#38037DepositorInsertNucleoporin 98 (NUP98 Human)
UseTagsHis-tag, 3CysteineExpressionBacterialMutationamino acids 1-498PromoterT7Available sinceSept. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBAD-eLACCO2.1
Plasmid#208017PurposeBiosensor for extracellular L-lactateDepositorInserteLACCO2.1
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRvUBC-mEGFP-NLS
Plasmid#205010PurposeThe vector contains 1kbp of the upstream and downstream regions of the ubiquitin c gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the ubiquitin c gene from R. varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHeACT-mEGFP-NLS
Plasmid#205003PurposeThe vector contains 1kbp of the upstream and downstream regions of the actin gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the actin gene from Hypsibius exemplaris
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCyRFP1-RhoA
Plasmid#84358Purposefusion protein of RhoA, FRET donorDepositorInsertmCyRFP1-RhoA (RHOA Human, Synthetic)
UseTagsmCyRFP1 N terminal fusion to RhoAExpressionMammalianMutationPromoterCMVAvailable sinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-HRas S17N
Plasmid#18665DepositorInsertH-Ras (HRAS Human)
UseTagsmonomeric EGFPExpressionMammalianMutationS17N mutation in H-RasPromoterAvailable sinceJuly 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
pME Actin Vhh mNeonGreen-HA-pA (JDW 1248)
Plasmid#224500PurposeGateway compatible middle entry clone containing Actin nanobody fused to mNeonGreen with an HA tag (Visualizing actin, actin chromobody)DepositorInsertActin-Vhh-mNeonGreen-HA-pA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only