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pHAGE-IRES-puro-NLS-dPguCas13b-3xEGFP-NLS-3xFlag

Plasmid

#132399

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Publication

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPguCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H151A; H1121A

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLHCX-Flag-mPKM2(C358S)

Plasmid

#42513

Depositor

Dimitrios Anastasiou

Publication

Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

Insert

Pyruvate Kinase M2 Cys358->Ser

Use

Retroviral

Tags

Flag

Mutation

Changed Cysteine 358 to Serine

Promoter

CMV

Available since

March 19, 2013

Availability

Academic Institutions and Nonprofits only
pCMV-HA-Ebp1

Plasmid

#67792

Purpose

Mammalian expression of Ebp1 tagged with HA

Depositor

Stephen Goff

Publication

Wang et al J Virol. 2014 Feb;88(3):1825-9. doi: 10.

Insert

Ebp1 (PA2G4 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only
hOT-GFP

Plasmid

#67848

Purpose

mammalian expression of human oxytocin receptor with GFP tag

Depositor

Christian Gruber

Publication

Koehbach et al Proc Natl Acad Sci U S A. 2013 Dec 24;11

Insert

oxytocin receptor (OXTR Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
LentiGuidPuro-hTP53_sgRNA-1

Plasmid

#88853

Purpose

CRISPR KO of Trp53

Depositor

Joan Massague

Publication

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp53

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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Mito-mGreenLantern

Plasmid

#164461

Purpose

Localization of mGreenLantern fluorescent protein to mitochondria

Depositor

Gregory Petsko

Publication

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Tags

Human COX8a first 29 amino acids

Expression

Mammalian

Mutation

Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

Promoter

CMV

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-tMCK-ER-TurboID

Plasmid

#149412

Purpose

Muscle-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Publication

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Insert

ER-TurboID

Use

AAV

Tags

HA and V5

Promoter

tMCK

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsXRN1_K

Plasmid

#146784

Purpose

Mammalian Expression of HsXRN1

Depositor

Elisa Izaurralde

Publication

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

HsXRN1 (XRN1 Human)

Expression

Mammalian

Available since

Nov. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-flag-YTHDF2-C

Plasmid

#52303

Purpose

Flag-tagged YTHDF2 C-terminal domain, from E384 to the end

Depositor

Chuan He

Publication

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTHDF2 (YTHDF2 Human)

Tags

flag

Expression

Mammalian

Mutation

Deleted amino acids 1-383

Promoter

T7

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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eBFP2 (eBFP2)_CD

Plasmid

#66034

Purpose

MoClo Basic Part: CDS - Fluorescent protein. Blue. Derived from Addgene#14891. [C:eBFP2:D]

Depositor

Douglas Densmore

Publication

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Fluorescent reporter - BFP

Use

Synthetic Biology

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-TBG-Cyto-TurboID

Plasmid

#149414

Purpose

Hepatocyte-specific expression of cytosolic TurboID, N-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Publication

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Insert

Cyto-TurboID

Use

AAV

Tags

V5

Promoter

TBG

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-16

Plasmid

#172746

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-16; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-16

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pTS96 pHAGE2 CMVtetO2 GFP-22xGS-SUMOEu-MCS

Plasmid

#199349

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOEu-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Publication

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP-SUMOEu

Expression

Mammalian

Mutation

WT

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMXS-hs-3xHA-LIN-41

Plasmid

#52717

Purpose

retroviral expression of human LIN-41 / TRIM71 with 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Publication

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCE27

Plasmid

#174405

Purpose

C. auris LEUpOUT NAT marker gRNA expression construct. Use with pCE35 CAS9 expression construct.

Depositor

Clarissa Nobile

Publication

Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

Insert

NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2

Use

CRISPR

Expression

Bacterial

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLV-mTurquoise-MLC-IRES-neo

Plasmid

#85145

Purpose

lentiviral expression of myosin light chain (MLC, MYL9), N-terminally tagged with mTurquoise

Depositor

Tobias Meyer

Publication

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

mTurquoise-MLC (myosin regulatory light chain, N-terminally tagged with mTurquoise (MYL9 Human)

Use

Lentiviral

Tags

mTurquoise

Promoter

EF1a

Available since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pErCas12a EZ Clone

Plasmid

#132641

Purpose

Expresses ErCas12a and an sgRNA to target a region of interest

Depositor

Stephen Ekker

Publication

Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

Insert

ErCas12a

Use

AAV and CRISPR

Tags

3x HA and SV40 NLS

Expression

Mammalian

Mutation

Q926R (Please see depositor comments) and BsaI si…

Promoter

CMV Promoter

Available since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-HA-RAPGEF2

Plasmid

#110162

Purpose

Expression of human RAPGEF2 in mammalian cells

Depositor

Daniele Guardavaccaro

Publication

Magliozzi et al Dev Cell. 2013 Dec 9;27(5):574-85. doi:

Insert

RAPGEF2 (Rap guanine nucleotide exchange factor 2 (RAPGEF2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCE35

Plasmid

#174409

Purpose

C. auris LEUpOUT NAT marker CAS9 expression construct. Use with pCE27 gRNA expression construct.

Depositor

Clarissa Nobile

Publication

Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

Insert

C. auris LEU2 1 of 2, pENO1, Cas9, NAT 1 of 2

Use

CRISPR

Expression

Bacterial

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Intron-Tagging-EGFP-Donor

Plasmid

#159740

Purpose

Contains EGFP flanked by a splice acceptor and a splice donor. Together with other intron tagging plasmids, it can be used to place the EGFP tag as a synthetic exon into introns of target genes.

Depositor

Stefan Kubicek

Publication

Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

Insert

EGFP

Use

Intron tagging donor

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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