We narrowed to 26,582 results for: CAL;

1. pGP-20XUAS-IVS-Syn21-jGCaMP8m-p10

   Plasmid

   #162387

   Purpose

   Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Insert

   jGCaMP8m
   

   Use

   Tags

   6xHis

   Expression

   Insect

   Mutation

   A25G F286Y

   Promoter

   hsp70

   Available since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8f-p10

   Plasmid

   #162385

   Purpose

   LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Insert

   jGCaMP8f
   

   Use

   Tags

   6xHis

   Expression

   Insect

   Mutation

   Q315L

   Promoter

   hsp70

   Available since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8m-p10

   Plasmid

   #162384

   Purpose

   LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Insert

   jGCaMP8m
   

   Use

   Tags

   6xHis

   Expression

   Insect

   Mutation

   A25G F286Y

   Promoter

   hsp70

   Available since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDF-Az

   Plasmid

   #197101

   Purpose

   To express the azidophenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into azidophenylalanine

   Depositor

   Kensaku Sakamoto

   Article

   Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20

   Insert

   TyrRS variant, amber suppressor tRNA, kanamycin resistance gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Thr32, Asn107, Pro158, Leu159, Gln162, Arg286 (Ty…

   Promoter

   Available since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQE80L-SpyCatcher-ELP-GFP

   Plasmid

   #69835

   Purpose

   Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do

   Insert

   SpyCatcher-ELP-GFP
   

   Use

   Tags

   6x His Tag, GFP, and TEV Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T5 promoter/lac operator element

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Mouse Alpha2 delta-2 HA pMT2

   Plasmid

   #58731

   Purpose

   expression of HA tagged mouse Alpha2 delta-2 in mamalian cells

   Depositor

   Annette Dolphin

   Article

   Davies et al J Neurosci. 2006 Aug 23;26(34):8748-57.

   Insert

   alpha2 delta-2 (Cacna2d2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pssGFP-hCRT

   Plasmid

   #80022

   Purpose

   Mammalian expression of signal peptide-EGFP-humanCRT

   Depositor

   Chinten James Lim

   Article

   Liu et al Cell Death Dis. 2016 Jun 16;7:e2268. doi

   Insert

   CRT (CALR Human)
   

   Use

   Tags

   EGFP and signal peptide

   Expression

   Mammalian

   Mutation

   signal peptide 17aa added before GFP-CRT, will ta…

   Promoter

   CMV

   Available since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

8. H2A-mTurquoise-RAC1-G12V-deltaCAAX

   Plasmid

   #176095

   Purpose

   Constitutively active, nuclear localized Rac1

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

   Insert

   RAC1 (RAC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G12V

   Promoter

   Available since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-CaMKIIa-SpyTag-C1C2-mCherry

   Plasmid

   #69833

   Purpose

   Lentiviral vector with CaMKIIa promoter and upstream CMV promoter expressing SpyTag-C1C2-mCherry fusion. SpyTag can be used to monitor membrane localization of the C1C2 opsin.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do

   Insert

   SpyTag-C1C2
   

   Use

   Lentiviral

   Tags

   Trafficking signal and mCherry

   Expression

   Mammalian

   Mutation

   Inserted SpyTag after the signal peptide of C1C2.

   Promoter

   CamKIIa

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGB3 alpha1 35s:PIF6:tNos (GB0170)

    Plasmid

    #160606

    Purpose

    TU for the constitutive expression of a chimeric construct comprising a DNA binding protein fused to A. thaliana PIF6 (amino acids 1-100) and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2022 Feb;20(2):399-4

    Insert

    PIF6
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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