Skip to main content
Addgene
Showing: 4751 - 4800 of 12997 results

  5. Per2:iLuc

    Plasmid
    #160293
    Purpose
    target Per2 gene with a color-switching luciferase cassette in mouse genome and the transfection of the recombinant DNA will lead genomic recombination and make a knock-in allele in mouse ES cells.
    Depositor
    Joseph Takahashi
    Article
    Shan et al Neuron. 2020 Aug 4. pii: S0896-6273(20)3
    Insert
    Per2LUC
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    no promotor
    Available since
    Dec. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    160293-plasmid-map-sequence-id-318636

    Enlarge

  8. pGL3 Pro Mut E2

    Plasmid
    #48750
    Purpose
    Expression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1) and SV40 promoter (backbone)
    Depositor
    Joseph Takahashi
    Article
    Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10
    Insert
    mPer2 promoter/enhancer region containing mutated E-box2 (-112 to +98)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Mutated E-box2 sequence from CACGTT to GCTAGT
    Promoter
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    48750-plasmid-map-sequence-id-448242

    Enlarge

  9. pSCL.175

    Plasmid
    #184996
    Purpose
    Express -Eco1 HEK3 editing ncRNA and gRNA
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1: HEK3 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Use
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    HEK3 donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    184996-plasmid-map-sequence-id-376621

    Enlarge

  12. pSCL.177

    Plasmid
    #184998
    Purpose
    Express -Eco1 EMX1 editing ncRNA and gRNA
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1: EMX1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Use
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    EMX1 donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    184998-plasmid-map-sequence-id-376623

    Enlarge

  14. pSCL.180

    Plasmid
    #185001
    Purpose
    Express -Eco1 AAVS1 editing ncRNA and gRNA
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1: AAVS1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Use
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    AAVS1 donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    185001-plasmid-map-sequence-id-376682

    Enlarge

  22. pBbB8k-csg-NbMS2

    Plasmid
    #171701
    Purpose
    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the coat protein of bacteriophage MS2 via a flexible linker.
    Depositor
    Neel Joshi
    Article
    Birnbaum et al ACS ES T Water. 2022 Nov 11;2(11):1836-1
    Insert
    csgBACEFG, with nanobody fused to CsgA
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171701-plasmid-map-sequence-id-336474

    Enlarge

  24. pSLIK-Neo-TGmiR-Luc

    Plasmid
    #25745
    Purpose
    Control lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA and GFP, constitutive Neomycin resistance gene coexpression.
    Depositor
    Iain Fraser
    Article
    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1
    Insert
    Luciferase miR-shRNA
    Use
    Lentiviral and RNAi
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 1, 2010
    Availability
    Academic Institutions and Nonprofits only
    25745-plasmid-map-sequence-id-12070

    Enlarge

  30. pSLIK-Venus-TmiR-Luc

    Plasmid
    #25739
    Purpose
    Control lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA, constitutive Venus fluorescent protein coexpression.
    Depositor
    Iain Fraser
    Article
    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1
    Insert
    Luciferase miR-shRNA
    Use
    Lentiviral and RNAi
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 1, 2010
    Availability
    Academic Institutions and Nonprofits only
    25739-plasmid-map-sequence-id-12064

    Enlarge

  36. pSLIK-Neo-TmiR-Luc

    Plasmid
    #25743
    Purpose
    Control lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA, constitutive Neomycin resistance gene coexpression.
    Depositor
    Iain Fraser
    Article
    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1
    Insert
    Luciferase miR-shRNA
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 1, 2010
    Availability
    Academic Institutions and Nonprofits only
    25743-plasmid-map-sequence-id-12068

    Enlarge

Showing: 4751 - 4800 of 12997 results