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Showing: 4751 - 4800 of 6616 results
  1. pCAG_SRGAP2A_GAP(R527L_K566A)

    Plasmid
    #222617
    Purpose
    Expresses full length SRGAP2 with R527L, K566A mt in the Rac1-specific GAP domain that abrogates the GTPase activating function of SRGAP2 without locking the GAP-domain in a form bound to Rac1-GDP.
    Depositor
    Insert
    SRGAP2 (SRGAP2 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Point mutations R527L and K566A (Rac1-specific GA…
    Available Since
    Oct. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. CAGGS-safb∆sap-NLS-3XFLAG-V5

    Plasmid
    #196091
    Purpose
    Expresses mouse SAFB lacking the n-terminal SAP domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag
    Depositor
    Insert
    SAFB (Safb Mouse)
    Tags
    C-terminal 3xFLAG and C-terminal V5
    Expression
    Bacterial and Mammalian
    Mutation
    Removed amino acids 31-65 (SAP domain) of mouse S…
    Promoter
    CAGGS
    Available Since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. CAGGS-safb∆rrm-NLS-3XFLAG-V5

    Plasmid
    #196093
    Purpose
    Expresses mouse SAFB lacking the rrm domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag
    Depositor
    Insert
    SAFB (Safb Mouse)
    Tags
    C-terminal 3xFLAG and C-terminal V5
    Expression
    Bacterial and Mammalian
    Mutation
    Removed amino acids 428-506 (RRM) of mouse SAFB p…
    Promoter
    CAGGS
    Available Since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. B52_puro_gRNA_3418711_KCNB2

    Plasmid
    #197558
    Purpose
    gRNA targeting upstream region of KCNB2 (site 3418711, control for epigenetic targeting)
    Depositor
    Insert
    upstream KCNB2 promoter gRNA (Kcnb2 Rat)
    Use
    Grna with puromycin selection
    Tags
    puromycin resistance cassette under CMV promotion
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    March 27, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. pJET-osr1-KI-donor

    Plasmid
    #184875
    Purpose
    Donor template for knock-in of p2a-CreERT2 into the medaka osr1 locus via CRISPR/Cas9-mediated homology directed repair
    Depositor
    Inserts
    Odd-Skipped Related Transcription Factor 1 5' homology arm
    Odd-Skipped Related Transcription Factor 1 3' homology arm
    p2a-CreERT2
    Myosin light chain 2 promoter
    EGFP
    Use
    CRISPR and Cre/Lox
    Available Since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. Ir7f-T2A-QF2 HDR plasmid

    Plasmid
    #140942
    Purpose
    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Ir7f gene
    Depositor
    Insert
    Ir7f-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir7f-right-HDR-arm
    Mutation
    4 point mutations (annotated on plasmid map) were…
    Available Since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
  7. pET15-His-∆NGlpG(C104V/D243G/L244G/F245G/M247C/S248G/M249G/A250G)

    Plasmid
    #155134
    Purpose
    Expresses N-terminally His-tagged E. coli GlpG C104V/D243G/L244G/F245G/M247C/S248G/M249G/A250G variant (residues 87-276) from pET15b
    Depositor
    Insert
    GlpG (glpG Escherichia coli)
    Tags
    His
    Expression
    Bacterial
    Mutation
    C104A/D243G/L244G/F245G/M247C/S248G/M249G/A250G, …
    Promoter
    T7
    Available Since
    Sept. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 4751 - 4800 of 6616 results