We narrowed to 11,061 results for: aga

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DNAJC11-EGFP

Plasmid

#210368

Purpose

Expresses DNAJC11 fused with EGFP in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

DNAJC11 (DNAJC11 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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NSP14-Flag

Plasmid

#210346

Purpose

Expresses SARS-CoV-2 NSP14 fused with Flag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)

Tags

Flag

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pACE-K2P10.1-StrepTagII

Plasmid

#191475

Purpose

Expresses K2P10.1 with C-terminal StrepTagII

Depositor

Arthur Laganowsky

Article

Zhu et al J Am Chem Soc. 2022 Apr 27;144(16):7048-

Insert

K2P10.1 (KCNK10, TREK-2 Human)

Expression

Insect

Mutation

N144Q (uniprot P57789-1)

Promoter

polH

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pUDP012

Plasmid

#101167

Purpose

E. coli/S. cerevisiae shuttle vector carrying amdS andSpcas9D147Y P411T and expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 and in S. pastorianus (HH-gRNASeILV6-HDV)

Depositor

Jean-Marc Daran

Article

de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

Insert

HH-gRNA-HDV targetting SeILV6 in S. pastorianus

Use

CRISPR

Expression

Yeast

Promoter

ScTDH3

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pSIL-eGFP-shACTN2

Plasmid

#52677

Purpose

shRNA against α-actinin-2 with eGFP transfection marker

Depositor

Johannes Hell

Article

Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

Insert

ACTN2 (Actn2 Rat)

Use

RNAi

Tags

CMV-EGFP

Promoter

U6

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only

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NFATc2-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75232

Purpose

CRISPR/Cas9 plasmid against human NFATc2

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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7432 Bicistronic_GFP_ires_puro

Plasmid

#64336

Purpose

This a retroviral expression plasmid expressing GFP along with puro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Insert

GFP

Use

Retroviral

Tags

GFP

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs-ms-Klf1

Plasmid

#50785

Purpose

retroviral expression of mouse Klf1

Depositor

Shinya Yamanaka

Article

Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

Insert

Klf1 (Klf1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pSico shSMYD3-C human

Plasmid

#85654

Purpose

expression of shRNA targeting human SMYD3

Depositor

Julien Sage

Article

Mazur et al Nature. 2014 Jun 12;510(7504):283-7. doi

Insert

shRNA targeting 3'UTR of SMYD3 (SMYD3 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-DNMT3a ts3

Plasmid

#115867

Purpose

DNMT3a knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

DNMT3a shRNA (DNMT3A Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-INTS3-N

Plasmid

#128416

Purpose

Express INTS3 N-termini (1-513 aa) in E. coli with a GST tag (N-terminal)

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

Insert

INTS3 (INTS3 Human)

Tags

GST

Expression

Bacterial

Mutation

N-terminal region (1–513 aa)

Promoter

tac promoter

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-2TK cyclin T1 (1-250) (P#467)

Plasmid

#14606

Depositor

Matija Peterlin

Article

Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

Insert

cyclin T1 (1-250) (CCNT1 Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 1-250

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pSV CDK9 (D167N) HA (P#601)

Plasmid

#14639

Depositor

Matija Peterlin

Article

Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

Insert

cdk9 D167N (CDK9 Human)

Tags

HA

Expression

Mammalian

Mutation

D167N. Kinase negative mutant.

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pCT-CMV-UBE3BΔHECT-copGFP-Puro

Plasmid

#107343

Purpose

Expresses UBE3B deletion of HECT domain fused to copGFP in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

UBE3B(ΔHECT)-copGFP (UBE3B Human)

Use

Lentiviral

Tags

copGFP

Expression

Mammalian

Mutation

deletion of HECT domain (757-end)

Promoter

CMV

Available Since

June 26, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pLV_mU6-sgNGN3_hU6-sgRNA_hUbC-PuroR-P2A-GFP

Plasmid

#162336

Purpose

Lentiviral expression of sgNGN3 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

S. pyogenes sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6; mU6

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLINK CDK9 myc (D167N) (P#1084)

Plasmid

#14637

Depositor

Matija Peterlin

Article

Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

Insert

cdk9 D167N (CDK9 Human)

Tags

myc

Expression

Mammalian

Mutation

D167N. Kinase negative mutant.

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pGEX-2TK cyclin T1 (1-551) (P#422)

Plasmid

#14609

Depositor

Matija Peterlin

Article

Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

Insert

cyclin T1 (1-551) (CCNT1 Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 1-551

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pET29a-hNABP2NhNABP1C

Plasmid

#128313

Purpose

Express chimeric protein hNABP2 N-terminus and hNABP1 C-terminus in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

chimeric protein hNABP2N+hNABP1C (NABP2, NABP1 Human)

Tags

His

Expression

Bacterial

Mutation

chimeric protein hNABP2N+hNABP1C, Y85C (please se…

Promoter

T7

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-cGASΔN C396/7A-HA

Plasmid

#130918

Purpose

Expresses human cGASΔN (aa160-522)-HA with C396/397A point mutation; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

cGASΔN C396/7A

Tags

HA

Expression

Mammalian

Mutation

mutated residues 396 and 397 to alanines

Promoter

CMV

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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Rev CDK9 (D167N) (P#858)

Plasmid

#14635

Depositor

Matija Peterlin

Article

Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

Insert

cdk9 D167N (CDK9 Human)

Tags

Rev

Expression

Mammalian

Mutation

D167N. Kinase negative mutant.

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only