We narrowed to 1,947 results for: 974

1. STK32B gRNA (BRDN0001146403)

   Plasmid

   #76373

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human STK32B

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   STK32B (STK32B Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. STK32B gRNA (BRDN0001145394)

   Plasmid

   #76374

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human STK32B

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   STK32B (STK32B Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. STK32B gRNA (BRDN0001144884)

   Plasmid

   #76375

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human STK32B

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   STK32B (STK32B Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PEA1-Nuclease

   Plasmid

   #176528

   Purpose

   Delivers all prime editing nuclease components in a single plasmid

   Depositor

   Paul Thomas

   Article

   Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

   Insert

   CbH-Cas9-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   GC to CA point mutation in SpCas9 to restore nucl…

   Promoter

   CbH for Cas9, hU6 for gRNAs

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lrrc4c-AP-His

   Plasmid

   #71959

   Purpose

   Expresses the extracellular region of the LRRC4C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Lrrc4c (Lrrc4c Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. PEA1-Puro

   Plasmid

   #171991

   Purpose

   Delivers all prime editing nickase components in a single, puromycin selectable plasmid

   Depositor

   Paul Thomas

   Article

   Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

   Insert

   CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV for Cas9, U6 for gRNAs

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PEA1-GFP

   Plasmid

   #171993

   Purpose

   Delivers all prime editing nickase components in a single, GFP selectable plasmid

   Depositor

   Paul Thomas

   Article

   Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

   Insert

   CbH-Cas9(H840A)-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV for Cas9, U6 for gRNAs

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. DHX37

   Plasmid

   #155539

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   DHX37 (DHX37 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRE3G-chimpanzee cGAS-HA

   Plasmid

   #186873

   Purpose

   Doxycycline-dependent expression of chimpanzee cGAS gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

   Insert

   Chimpanzee cGAS with C-terminal HA tag (CGAS Pan troglodytes)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   TRE3G

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 5'-3xFLAG Kansl3+exon minimal CMV pCDNA5

    Plasmid

    #212053

    Purpose

    LR vector for integration of Kansl3+exon(78nt) into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Kansl3+exon(78nt) (Kansl3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pOTTC1663 - pAAV SYN1 mGas6-Myc-DDK

    Plasmid

    #202540

    Purpose

    An adeno-associated viral vector expressing murine Gas6 fused Myc and DDK epitopes from a synapsin promoter

    Depositor

    Christopher Richie

    Article

    Owlett et al J Neuroinflammation. 2022 Feb 7;19(1):38

    Insert

    Gas6 (Gas6 Mouse)
    

    Use

    AAV

    Tags

    Myc-DDK

    Expression

    Mutation

    Promoter

    SYN1

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223_SERPINB4_WT

    Plasmid

    #81766

    Purpose

    Gateway Donor vector containing SERPINB4 , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SERPINB4 (SERPINB4 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PEA1-Nuclease-Puro

    Plasmid

    #171992

    Purpose

    Delivers all prime editing nuclease components in a single, puromycin selectable plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    GC to CA point mutation in SpCas9 to restore nucl…

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PEA1-Nuclease-GFP

    Plasmid

    #171994

    Purpose

    Delivers all prime editing nuclease components in a single, GFP selectable plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    GC to CA point mutation in SpCas9 to restore nucl…

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTRE3G-chimpanzee cGASdeltaN-HA

    Plasmid

    #186874

    Purpose

    Doxycycline-dependent expression of chimpanzee cGAS gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Chimpanzee cGAS truncation mutant (160-520 a.a.) with C-terminal HA tag (CGAS Pan troglodytes, Synthetic)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    N-terminal truncation

    Promoter

    TRE3G

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTRE3G-chimpanzee cGASdeltaN-GFP

    Plasmid

    #186847

    Purpose

    Doxycycline-dependent expression of chimpanzee cGAS gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Chimpanzee cGAS truncation mutant (160-520 a.a.) with C-terminal GFP tag (CGAS Pan troglodytes, Synthetic)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    N-terminal truncation

    Promoter

    TRE3G

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOTTC1664 - pAAV SYN1 mGas6(delta)-Myc-DDK

    Plasmid

    #202541

    Purpose

    An adeno-associated viral vector expressing murine Gas6 with deletion of (F50-E275) fused Myc and DDK epitopes from a synapsin promoter

    Depositor

    Christopher Richie

    Article

    Owlett et al J Neuroinflammation. 2022 Feb 7;19(1):38

    Insert

    Gas6 (Gas6 Mouse)
    

    Use

    AAV

    Tags

    Myc-DDK

    Expression

    Mutation

    Deletion of F50-E275

    Promoter

    SYN1

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKLV2-U6(gDmap1 v4)-PGK-Puro-BFP

    Plasmid

    #117143

    Purpose

    Express gRNA vs Dmap1

    Depositor

    Kosuke Yusa

    Article

    Yu et al Stem Cells. 2019 Apr 1. doi: 10.1002/ste

    Insert

    gDmap1 v4 (Dmap1 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    human U6 promoter, mouse PGK promoter

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKLV2-U6(gDmap1 v5)-PGK-Puro-BFP

    Plasmid

    #117144

    Purpose

    Express gRNA vs Dmap1

    Depositor

    Kosuke Yusa

    Article

    Yu et al Stem Cells. 2019 Apr 1. doi: 10.1002/ste

    Insert

    gDmap1 v5 (Dmap1 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    human U6 promoter, mouse PGK promoter

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKLV2-U6(gDmap1 v2)-PGK-Puro-BFP

    Plasmid

    #117141

    Purpose

    Express gRNA vs Dmap1

    Depositor

    Kosuke Yusa

    Article

    Yu et al Stem Cells. 2019 Apr 1. doi: 10.1002/ste

    Insert

    gDmap1 v2 (Dmap1 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    human U6 promoter, mouse PGK promoter

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge