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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 4,083 results for: Mcc;

Showing: 1921 - 1940 of 4083 results

  1. pSMT3-FBF2 AS/Y

    Plasmid
    #166974
    Purpose
    Expresses FBF-2 with AS/Y mutations in repeat 5 in E. coli for purification and crystallization studies
    Depositor
    Traci Hall
    Article
    Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1
    Insert
    FBF-2 (fbf-2 Nematode)
    Use
    Tags
    His-SUMO
    Expression
    Bacterial
    Mutation
    FBF-2 PUM RNA binding domain; amino acids 164-575…
    Promoter
    T7
    Available since
    April 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    166974-plasmid-map-sequence-id-331632

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  7. pPICZ-His6-K2P4.1B-mCherry-StreptagII

    Plasmid
    #158744
    Purpose
    Expresses K2P4.1 isoform B with TEV protease cleavable N- and C-terminal affinity tags.
    Depositor
    Arthur Laganowsky
    Article
    Schrecke et al Nat Chem Biol. 2021 Jan;17(1):89-95. doi
    Insert
    K2P4.1-B (KCNK4 Human)
    Use
    Tags
    His6 and mCherry with Streptag II
    Expression
    Yeast
    Mutation
    Residues 1-290 and N-linked glycosylation sites r…
    Promoter
    AOX1
    Available since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    158744-plasmid-map-sequence-id-309639

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  11. GPR152-Tango

    Plasmid
    #66328
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    GPR152 (GPR152 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. ADRA2A-Tango

    Plasmid
    #66216
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    ADRA2A (ADRA2A Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66216-plasmid-map-sequence-id-417865

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  14. CXCR7-Tango

    Plasmid
    #66265
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    CXCR7 (ACKR3 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. pcDNA3.1-GGamma2-GFP2

    Plasmid
    #166774
    Purpose
    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GGamma2-GFP2 (GNG2 Synthetic, Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Contains an N-terminal GFP2 and GSAG linker
    Promoter
    CMV
    Available since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    166774-plasmid-map-sequence-id-330993

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  17. ADRB2-Tango

    Plasmid
    #66220
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    ADRB2 (ADRB2 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66220-plasmid-map-sequence-id-247680

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  20. OPRK1-Tango

    Plasmid
    #66462
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    OPRK1 (OPRK1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66462-plasmid-map-sequence-id-157905

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Showing: 1921 - 1940 of 4083 results