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pFLAG-Cherry-N1-PRKAG1

Plasmid

#30308

Depositor

Jay Brenman

Article

Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

Insert

AMP-activated Protein Kinase γ1 (PRKAA1 Human)

Tags

FLAG and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2011

Availability

Academic Institutions and Nonprofits only
GALT-APEX2

Plasmid

#215554

Purpose

Stable, inducible expression in Golgi

Depositor

Nevan Krogan

Article

Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

Insert

APEX2-NES-GalT-EGFP

Use

Lentiviral

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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negMaLionB

Plasmid

#113911

Purpose

negative control for Blue ATP sensor

Depositor

Tetsuya Kitaguchi

Article

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Insert

negMaLionB

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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Cyto-APEX2

Plasmid

#215552

Purpose

Stable, inducible expression in cytoplasm

Depositor

Nevan Krogan

Article

Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

Insert

EGFP-Flag tag-APEX2-NES

Use

Lentiviral

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pJYScr

Plasmid

#132719

Purpose

Plasmid for an easy-to-construct crRNA delivery vector using hybridized oligonucleotides. Constitutive transcription of crRNA, Spectinomycin resistance

Depositor

Jan Marienhagen

Article

Krumbach et al ACS Synth Biol. 2019 Dec 11. doi: 10.102

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pQFT-mNGsacB

Plasmid

#203676

Purpose

pQFT with mNeonGreen and sacB under control of PQJ

Depositor

Urs Jenal

Article

Klotz et al Appl Environ Microbiol. 2023 May 18:e002

Inserts

mNeonGreen
sacB

Expression

Bacterial

Mutation

Has synonymous (silent) mutations to remove BsrGI…

Promoter

PQJ

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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Endo-APEX2

Plasmid

#215553

Purpose

Stable, inducible expression in endosome

Depositor

Nevan Krogan

Article

Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

Insert

EGFP-2xFYVE-APEX2-NES

Use

Lentiviral

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-PTS1-IRES-Puromycin

Plasmid

#134665

Purpose

Expresses EGFP-tagged peroxisome marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

tripeptide SKL (Peroxisome targeting signal 1)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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iRFP-FRB-ER

Plasmid

#139317

Purpose

endoplasmic reticulum-resident FRB for chemically induced dimerization

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

iRFP:MTOR(2021-2113):[GGSA]2ILNSRV:SACM1L(521-587)

Expression

Mammalian

Available Since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-mBAF45a IRES GFP

Plasmid

#17880

Depositor

Jerry Crabtree

Article

Lessard et al Neuron. 2007 Jul 19. 55(2):201-15.

Insert

BAF45a (Phf10 Mouse)

Tags

FLAG and IRES EGFP

Expression

Mammalian

Available Since

May 13, 2008

Availability

Academic Institutions and Nonprofits only

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PM-APEX2

Plasmid

#215557

Purpose

Stable, inducible expression in plasma membrane

Depositor

Nevan Krogan

Article

Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

Insert

Lyn11-EGFP-Flag tag-APEX2-NES

Use

Lentiviral

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pACAGW-ChR2-Venus-AAV

Plasmid

#20071

Purpose

AAV-mediated expression of ChR2-Venus for neuronal excitation

Depositor

Karel Svoboda

Article

Leopoldo et al Nature. 2009 Jan 18.

Has Service

AAV1 and AAV9

Insert

Channelrhodopsin-2 (CHLRE_02g085257v5 Chlamydomonas reinhardtii)

Use

AAV

Tags

Venus

Expression

Mammalian

Promoter

CAGG

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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CMV-HA-hTAF4

Plasmid

#17983

Depositor

Naoko Tanese

Article

Tanese et al Proc Natl Acad Sci U S A. 1996 Nov 26. 9

Insert

TAF4 (TAF4 Human)

Tags

HA

Expression

Mammalian

Mutation

missing 5' end

Available Since

Aug. 19, 2008

Availability

Academic Institutions and Nonprofits only
pET-Sfp R4-4 mutant

Plasmid

#173491

Purpose

Expression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3′-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins.

Depositor

Jun Yin

Article

Sunbul et al J Mol Biol. 2009 Apr 10;387(4):883-98. d

Insert

Sfp-R44

Tags

His tag

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pTol-Promoter:NLS-tdMCP-eGFP

Plasmid

#86248

Purpose

For generating transgenic lines that ubiquitously expressing the fluorescent MCP reporter

Depositor

Florence Marlow

Article

Campbell et al Development. 2015 Apr 1;142(7):1368-74.

Insert

beta Actin promoter:NLS-tdMCPeGFP

Use

Used with transposase to make transient or stable…

Tags

NLS-tdMCP-eGFP

Promoter

Beta Actin

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MAC_C_AOX

Plasmid

#111661

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

AOX

Expression

Mammalian

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19_MTF2_HTC3xF_Puro_donor

Plasmid

#246392

Purpose

MTF2 donor plasmid with puromycin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

MTF2 (MTF2 Human)

Tags

HaloTag_3XFLAG

Expression

Mammalian

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_PHF1_KO_BSD_donor

Plasmid

#246396

Purpose

PHF1 KO donor plasmid with blasticidin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

PHF1 (PHF1 Human)

Expression

Mammalian

Mutation

KO

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_PHF19_HTC3xF_BSD_donor

Plasmid

#246398

Purpose

PHF19 donor plasmid with blasticidin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

PHF19 (PHF19 Human)

Tags

HaloTag_3XFLAG

Expression

Mammalian

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_PHF19_KO_BSD_donor

Plasmid

#246400

Purpose

PHF19 KO donor plasmid with blasticidin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

PHF19 (PHF19 Human)

Expression

Mammalian

Mutation

KO

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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