We narrowed to 2,463 results for: T2A

1. sgRNA1_NANOG

   Plasmid

   #64153

   Purpose

   Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA1_NANOG (NANOG Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-pEF1a-rtTAc

   Plasmid

   #172121

   Purpose

   Constitutive expression of InteinC-rtTAC

   Depositor

   Chun Xu

   Article

   Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

   Insert

   InteinC-rtTAc
   

   Use

   AAV

   Promoter

   EF1a

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. MTK3_027

   Plasmid

   #123742

   Purpose

   Encodes bicistronic ABI gene expression system as a Type 3 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   VP16::PYLcs-T2A-GAL4DBD::ABIcs
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPN461

   Plasmid

   #137877

   Purpose

   PiggyBac vector for expression of 1x gRNA targeting POGZ for CRISPRi; mRFP-T2A-BlasticidinR

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   POGZ (POGZ Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPN460

   Plasmid

   #137878

   Purpose

   PiggyBac vector for expression of 1x gRNA targeting POGZ for CRISPRa;mRFP-T2A-BlasticidinR

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   POGZ (POGZ Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLQ1371-sgYAP-2

   Plasmid

   #121423

   Purpose

   sgYAP-2 sequence: GAGATGACTTCCTGAACAGTG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

   Depositor

   Ovijit Chaudhuri, Stanley Qi

   Article

   Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

   Insert

   sgYAP-2
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   April 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. sgRNA4_NANOG

   Plasmid

   #64156

   Purpose

   Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA4_NANOG (NANOG Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. sgRNA3_NANOG

   Plasmid

   #64155

   Purpose

   Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA3_NANOG (NANOG Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. sgRNA2_NANOG

   Plasmid

   #64154

   Purpose

   Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA2_NANOG (NANOG Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. Split PS Intein gal4-vp16

    Plasmid

    #242030

    Purpose

    Blue-light-controlled release of gal4-vp16 transactivator from the cytoplasm to the nucleus. Split version.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

    Insert

    Split PS Intein gal4-vp16
    

    Expression

    Mammalian

    Mutation

    pNL3.2.NF-KB-RE[NlucP/NF-KB-RE/Hygro]

    Promoter

    CMV

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBIRC5-Split PS Intein-N tTA

    Plasmid

    #242033

    Purpose

    N-terminal segment of the blue-light-controlled split PS Intein tTA gene expression system, co-expressing mTagBFP2 under the BIRC5 promoter.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

    Insert

    Split PS Intein-N tTA
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    The CMV promoter was replaced with the BIRC5 prom…

    Promoter

    hTERT

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. phTERT-Split PS Intein-N tTA

    Plasmid

    #242034

    Purpose

    N-terminal segment of the blue-light-controlled split PS Intein tTA gene expression system, co-expressing mTagBFP2 under the hTERT promoter.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

    Insert

    Split PS Intein-N tTA
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    The CMV promoter was replaced with the hTERT prom…

    Promoter

    hTERT

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQAT26

    Plasmid

    #223398

    Purpose

    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and the sgRNA was driven by AtU3; BASTA for plants select.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQAT28

    Plasmid

    #223400

    Purpose

    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and sgRNA was driven by OsU3; Hygromycin for plant select.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-OsU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYPQAT29

    Plasmid

    #223401

    Purpose

    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and the sgRNA was driven by OsU3; BASTA for plant select.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-OsU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYPQAT30

    Plasmid

    #223402

    Purpose

    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 and the sgRNA was driven by separate ZmUbi1; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-ZmUbi-gRNA scaffold 2.0-tRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19-whiteCopyCatcher

    Plasmid

    #174062

    Purpose

    CopyCatcher insertion donor for the white locus

    Depositor

    Ethan Bier

    Article

    Li et al Nat Commun. 2021 May 11;12(1):2625. doi:

    Insert

    white (w Fly, Synthetic)
    

    Expression

    Bacterial

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. MTK4a_010

    Plasmid

    #123823

    Purpose

    Encodes a nuclear localized mRuby2 coexpression cassette as a Type 4a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    T2A::NLS::mRuby2
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. MTK4a_011

    Plasmid

    #123824

    Purpose

    Encodes a nuclear localized iRFP670 coexpression cassette as a Type 4a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    T2A::NLS::iRFP670
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. MTK4a_021

    Plasmid

    #123834

    Purpose

    Encodes a nuclear localized and destabilized mRuby2 coexpression cassette as a Type 4a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    T2A::NLS::mRuby2::PEST4d
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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