We narrowed to 13,177 results for: ckb

1. pBK2043-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9

   Plasmid

   #223163

   Purpose

   2nd gen. AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310 (MECP2)

   Promoter

   Available since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGA-red-mini

   Plasmid

   #196338

   Purpose

   Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening

   Depositor

   Paola Branduardi

   Article

   Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology; Golden gate assembly backbone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGA-red-maxi

   Plasmid

   #196337

   Purpose

   Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening

   Depositor

   Paola Branduardi

   Article

   Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology; Golden gate assembly backbone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGA-blue

   Plasmid

   #196336

   Purpose

   Easy-MISE toolkit Level 0 destination plasmid for parts cloning with Esp3I-based Golden Gate Assembly; allowing fast postitive clones test with AmilCP chromoprotein-based blue/white screening

   Depositor

   Paola Branduardi

   Article

   Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

   Type

   Empty backbone

   Use

   Synthetic Biology; Golden gate assembly backbone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKSB-AeU6-1gRNA

   Plasmid

   #183912

   Purpose

   gRNA-expressing plasmid under Aedes aegypti AAEL017702 U6 promoter

   Depositor

   Eric Marois

   Article

   Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

   Insert

   gRNA scaffold for protospacer cloning into BbsI sites
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Aedes aegypti U6 promoter (AAEL017702)

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKSB-AeU6-2gRNA

   Plasmid

   #183913

   Purpose

   gRNA-expressing plasmid under Aedres aegypti AAEL017774 U6 promoter

   Depositor

   Eric Marois

   Article

   Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

   Insert

   gRNA scaffold for protospacer cloning into BbsI sites
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Aedes aegypti U6 promoter (AAEL017774)

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGMC00008

   Plasmid

   #166724

   Purpose

   sgRNA cloning backbone

   Depositor

   Raj Chari

   Article

   Choi et al Nat Commun. 2020 Jun 1;11(1):2718. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEMS2001

   Plasmid

   #105871

   Purpose

   High copy HRPT backbone plasmid for insertion of MiniP promoters

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 22, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pMOD_B3200

   Plasmid

   #91079

   Purpose

   Module B, Promoter: none (begins with P2A to be fused to TALE-VPR_1), Gene: TALE-VPR_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TALE-VPR_2 backbone with Esp3I ccdb cassette for repeat cloning
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   none (begins with P2A to be fused to TALE-VPR_1)

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMOD_B3400

    Plasmid

    #91080

    Purpose

    Module B, Promoter: none (begins with P2A to be fused to TALE-VP64_1), Gene: TALE-VP64_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TALE-VP64_2 backbone with Esp3I ccdb cassette for repeat cloning
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    none (begins with P2A to be fused to TALE-VP64_1)

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMOD_A3101

    Plasmid

    #91048

    Purpose

    Module A, Promoter: 35S, Gene: TALE-VPR_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALE-VPR_ 2)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TALE-VPR_1 backbone with Esp3I ccdb cassette for repeat cloning
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    35S

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMOD_A3301

    Plasmid

    #91049

    Purpose

    Module A, Promoter: 35S, Gene: TALE-VP64_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALE-VP64_ 2)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TALE-VP64_1 backbone with Esp3I ccdb cassette for repeat cloning
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    35S

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAV10.K3.loxP

    Plasmid

    #63202

    Purpose

    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAV10.K6.loxP

    Plasmid

    #63203

    Purpose

    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAV10.K5.loxP

    Plasmid

    #63204

    Purpose

    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAV10.HO3.loxP

    Plasmid

    #63205

    Purpose

    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAV10.HO6

    Plasmid

    #63211

    Purpose

    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into HO locus (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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18. PX458 V3

    Plasmid

    #226957

    Purpose

    CBh-SpCas9-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLQ1658-dCas9-EGFP

    Plasmid

    #51023

    Purpose

    Template for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system)

    Depositor

    Bo Huang, Stanley Qi

    Article

    Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

    Insert

    dCas9 fuse to EGFP
    

    Use

    CRISPR and Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR promoter

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pB PUb-integrase

    Plasmid

    #183966

    Purpose

    piggyBac plasmid for insect transformation, expressing PhiC31 integrase under control of the Aedes aegypti PUb promoter, GFP fluorescent marker

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

    Insert

    PhiC31 integrase
    

    Use

    Piggybac insect transgenesis destination vector

    Tags

    Expression

    Mutation

    Promoter

    Aedes aegypti Polyubiquitin

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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