We narrowed to 9,845 results for: Coli

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pET28a-PTBP1-RRM12CCtoSS

Plasmid

#89155

Purpose

Recombinant expression of PTBP1-RRM12 C250S,C251S in E.coli

Depositor

Frédéric Allain

Article

Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

Insert

Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1+2 mutant C250S, C251S (PTBP1 Human)

Tags

hexa His

Expression

Bacterial

Mutation

mutations: Cys 250 to Ser, Cys 251 to Ser

Promoter

T7

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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H14-MBP-SUMO-Cys-Linker-BAF_L58R

Plasmid

#101780

Purpose

Expresses His-MBP-tagged mutant BAF (L58R) in E.coli (SUMO-cleavable)

Depositor

Daniel Gerlich

Article

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

BAF (BANF1) (BANF1 Human)

Tags

His14-MBP-SUMO

Expression

Bacterial

Mutation

L58R mutation

Promoter

T5

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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C-His-GvpC-Calpain

Plasmid

#153295

Purpose

Expresses calpain-sensitive variant of Anabaena flos-aquae GvpC in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Insert

Calpain-sensitive Gas vesicle protein C (his-tagged)

Tags

His-tag

Expression

Bacterial

Mutation

1) Contains an extra glycine after the methionine…

Promoter

T7

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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delNC C-His Ana GvpC in pET28a

Plasmid

#85736

Purpose

Expresses a truncated His-tagged GvpC from Anabaena flos-aquae without the N-and C-terminal regions in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

Insert

Gas vesicle protein C (truncated and his-tagged)

Tags

His-tag

Expression

Bacterial

Mutation

Deletion of N and C terminal regions, addition of…

Promoter

T7

Available Since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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GB1-His-TET1-878-911(pL91)

Plasmid

#124390

Purpose

E.Coli produced TET1 SID (TET1-878-911) purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

GB1-His-TET1-878-911 wildtype (TET1 Human)

Tags

GB1-Histidine

Expression

Bacterial

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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Kv7.4 AB (325-557, Δ 368-523)/pET28HMT

Plasmid

#111052

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Tags

His Tag and MBP tag

Expression

Bacterial

Mutation

Kv7.4 AB (325-557, Δ 368-523)

Promoter

T7

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMEL11

Plasmid

#107917

Purpose

amdS based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCAD123_hIrg1_N152K,M154I_pvp008

Plasmid

#239118

Purpose

hACOD1 with N152K and M154I mutations in E.coli expression vector

Depositor

Konrad Buessow

Article

Chen et al Sci Rep. 2023 Jun 26;13(1):10360. doi: 1

Insert

cis-aconitate decarboxylase (ACOD1 Human)

Tags

StrepTag and TEV site

Expression

Bacterial

Mutation

Deleted amino acids 1-3 and 462-end. Mutated N152…

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pColdI-SBP-DDX3_helicase_core

Plasmid

#233622

Purpose

Expression of SBP-tagged helicase core region of DDX3X in E.coli

Depositor

Shintaro Iwasaki

Article

Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

Insert

SBP-DDX3X helicase core (EIF4A1 Human)

Tags

His6-SBP

Expression

Bacterial

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET-NPR4 (SA binding domain)

Plasmid

#226885

Purpose

Expresses the Salicylic acid binding core (SBC) of NPR4 in E.coli, in an insoluble form

Depositor

Ning Zheng

Article

Wang et al Nature. 2020 Oct;586(7828):311-316. doi:

Insert

The Salicylic acid (SA) binding domain of NPR4 (NPR4 Mustard Weed)

Tags

His tag

Expression

Bacterial

Mutation

NPR4 SA-binding core (residues 373-516), with a s…

Promoter

T7

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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