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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 10,599 results for: cat.1

Showing: 3881 - 3920 of 10599 results

  1. pGEX-HA-GlpG(D243G/L244G/F245G/M247G/S248G/M249G/A250G)

    Plasmid
    #155136
    Purpose
    Expresses N-terminally GFP- and HA-tagged E. coli GlpG D243G/L244G/F245G/M247G/S248G/M249G/A250G variant from pGEX 4P-1
    Depositor
    Sinisa Urban
    Article
    Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910
    Insert
    GlpG (glpG Escherichia coli)
    Use
    Tags
    GST and HA
    Expression
    Bacterial
    Mutation
    D243G/L244G/F245G/M247G/S248G/M249G/A250G
    Promoter
    tac
    Available since
    Sept. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    155136-plasmid-map-sequence-id-310495

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  6. HA-HA-AR-DBD-LBD

    Plasmid
    #171237
    Purpose
    Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain and Ligand-binding domain (DBD-LBD)
    Depositor
    Steven Balk
    Article
    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do
    Insert
    2xHA-tagged human androgen receptor trancate:DNA-binding domain and Ligand-binding domain (AR Human)
    Use
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    AR truncate
    Promoter
    CMV
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    171237-plasmid-map-sequence-id-336036

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  7. HA-HA-AR-NTD-DBD

    Plasmid
    #171236
    Purpose
    Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR N-terminal-domain and DNA-bindind domain (NTD-DBD)
    Depositor
    Steven Balk
    Article
    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do
    Insert
    2xHA-tagged human androgen receptor trancate:N-terminal-domain and DNA-binding domain (AR Human)
    Use
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Mutation
    AR truncate
    Promoter
    CMV
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    171236-plasmid-map-sequence-id-336032

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  8. SARS-CoV-2 Nucleocapsid-3xHA-IRES-mcherry

    Plasmid
    #173030
    Purpose
    To express SARS-CoV-2 Nucleocapsid protein tagged with 3xHA and in frame with mcherry (NCP-IRES-mcherry) in mammalian cells
    Depositor
    Mohamed Fareh
    Article
    Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:
    Insert
    SARS-CoV-2 Nucleocapsid phosphoprotein
    Use
    Lentiviral
    Tags
    3xHA and IRES-mcherry
    Expression
    Mammalian
    Mutation
    Promoter
    Gag (truncated)
    Available since
    Sept. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    173030-plasmid-map-sequence-id-341538

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  11. Flag-neo-PP1-Alpha-H248K

    Plasmid
    #171268
    Purpose
    Mammalian expression of 3x-Flag-tagged human PP1 alpha catalytic subunit H248K mutant that loses enzymatic activity
    Depositor
    Steven Balk
    Article
    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do
    Insert
    3x-Flag-tagged human PP1 alpha catalytic subunit H248K mutant (PPP1CA Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    PPP1CA-His248Lys (H248K)
    Promoter
    CMV
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    171268-plasmid-map-sequence-id-336095

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  13. p426_Cas9_gRNA-YPRCd15c

    Plasmid
    #87404
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting YPRCd15c sequence AATCCGAACAACAGAGCATA in yeast chromosome 14.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting YPRCd15c
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87404-plasmid-map-sequence-id-193447

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  25. pET28a-his-Gluc-iLID

    Plasmid
    #172096
    Purpose
    Expresses a fusion protein of Gluc and iLID, which dimerizes with a sspB-tagged protein when the bioluminescent reaction is initiated, in bacteria
    Depositor
    Avi Schroeder
    Article
    Adir et al Nat Commun. 2022 Apr 28;13(1):2328. doi:
    Insert
    Gaussia Luciferase - iLID
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Gluc Mutations - M43L, M110L
    Promoter
    T7
    Available since
    April 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    172096-plasmid-map-sequence-id-338563

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  31. pETDuet-msGC-NT21

    Plasmid
    #78102
    Purpose
    expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta
    Depositor
    William Montfort
    Article
    Fritz et al Biochemistry. 2013 Mar 5;52(9):1568-82.
    Inserts
    fragment of alfa subunit of sGC
    fragment of beta subunit of sGC
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    fragment 1-380 of the beta subunit and fragment 2…
    Promoter
    T7
    Available since
    June 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    78102-plasmid-map-sequence-id-151428

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  32. pETDuet-msGC-NT13

    Plasmid
    #75087
    Purpose
    expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta
    Depositor
    William Montfort
    Article
    Fritz et al Biochemistry. 2013 Mar 5;52(9):1568-82.
    Inserts
    fragment of alfa subunit of sGC
    fragment of beta subunit of sGC
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    fragment 1-380 of the beta subunit and fragment 4…
    Promoter
    Available since
    May 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    75087-plasmid-map-sequence-id-148282

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  33. pcDNA3 RL3m6L2

    Plasmid
    #109366
    Purpose
    Human rod opsin chimera with intracellular loop 3 replaced by intracellular loop 2 of mGluR6 with 1D4 tag
    Depositor
    Robert Lucas
    Article
    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.
    Insert
    Rhodopsin chimera with mGluR6 intracellular loop 2 at intracellular loop 3 position (GRM6, RHO Human)
    Use
    Tags
    1D4
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    109366-plasmid-map-sequence-id-210142

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  34. p426_Cas9_gRNA-YOLCd1b

    Plasmid
    #87401
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting YOLCd1b sequence GACTAGTTAAGCGAGCATGT in yeast chromosome 15.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting YOLCd1b
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87401-plasmid-map-sequence-id-193444

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  40. pKTop::sptse5-CT (1169-1229)

    Plasmid
    #192948
    Purpose
    For membrane insertion study; expresses Tse5-CT (1169-1229)/PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus
    Depositor
    David Albesa-Jové
    Article
    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:
    Insert
    sptse5-CT (1169-1229)
    Use
    Tags
    PelB signal peptide
    Expression
    Bacterial
    Mutation
    encodes for tse5 (1169-1229)
    Promoter
    Available since
    Dec. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    192948-plasmid-map-sequence-id-380616

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Showing: 3881 - 3920 of 10599 results