We narrowed to 8,775 results for: construct

Showing: 3881 - 3920 of 8775 results

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KTK_136

Plasmid

#180520

Purpose

Empty backbone for D1.1 cloning, contains sfGFP dropout

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCheck2 Dest (R1-R2) #BGV129

Plasmid

#48955

Purpose

Destination dual luciferase reporter plasmid for checking effectiveness of shRNA. Compatible with standard AttL1-AttL2 Gateway Entry vectors.

Depositor

David Dankort

Article

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Type

Empty backbone

Use

Luciferase; Gateway cloning vector

Promoter

SV40

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
MTK3b_004

Plasmid

#123785

Purpose

Encodes mAzamiGreen as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

mAzamiGreen

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pGK:HygroR-CMV:mKO2-C_UTR

Plasmid

#68481

Purpose

Lentivirus expressing mKO2 with UTR sensor cassette and hygromycin resistance

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Hygromycin Resistance
mKO2-C_UTR

Use

Lentiviral

Expression

Mammalian

Mutation

shRNA sensor cassette "B" added to 3&#x…

Promoter

CMV and pGK

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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MTK2_013

Plasmid

#123705

Purpose

Encodes a spacer (for multicistronic) as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pSpacer multicistronic

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3_039

Plasmid

#123753

Purpose

Encodes mouse RelA as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

RelA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK5_009

Plasmid

#123853

Purpose

Encodes ConR6 as a Type 5 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector R6

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK5_010

Plasmid

#123854

Purpose

Encodes ConR7 as a Type 5 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector R7

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK4a_003

Plasmid

#123817

Purpose

Encodes Nuclear import sequence as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

NLS

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM773a/b

Plasmid

#84141

Purpose

target miRNA773a/b for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM773a/b

Expression

Plant

Promoter

2x35S

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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KTK_001

Plasmid

#180517

Purpose

Empty backbone for creation of entry parts for the KTK, contains sfGFP dropout

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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MTK3_002

Plasmid

#123719

Purpose

Encodes bsr (blasticidin-S deaminase) as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Blasticydin Resistance

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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KTK_247

Plasmid

#180529

Purpose

Entry vector containing pTac promoter

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

pTac promoter

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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MTK2_016

Plasmid

#123708

Purpose

Encodes truncated pCMV as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pCMV-A

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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p3E-FRB(PLF)-HA no-pA

Plasmid

#82597

Purpose

3' entry vector containing HA epitope tag fused to C-terminus of FRB mutant (K2095P, T2098L, W2101F) without pA for rapamycin/rapalog induced dimerization

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

FRB-HA (no pA)

Mutation

K2095P, T2098L, W2101F

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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MTK4b_004

Plasmid

#123843

Purpose

Encodes spacer sequence as a Type 4b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Spacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK2_017

Plasmid

#123709

Purpose

Encodes truncated pCMV as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pCMV-B

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK4b_002

Plasmid

#123841

Purpose

Encodes SV40 polyadenylation signal as a Type 4b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

SV40 pA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK4a_005

Plasmid

#123819

Purpose

Encodes Mitochondria Targeting Sequence as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

MTS

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK4a_007

Plasmid

#123821

Purpose

Encodes Peroxisome Targeting Sequence as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PTS1

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK4a_002

Plasmid

#123816

Purpose

Encodes Nuclear Export sequence as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

NES

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3a_002

Plasmid

#123755

Purpose

Encodes Nuclear Export sequence as a Type 3a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

NES

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3a_003

Plasmid

#123756

Purpose

Encodes Nuclear import sequence as a Type 3a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

NLS

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3a_004

Plasmid

#123757

Purpose

Encodes Golgi Targeting Sequence as a Type 3a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

GTS

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK3a_005

Plasmid

#123758

Purpose

Encodes Mitochondria Targeting Sequence as a Type 3a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

MTS

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3a_013

Plasmid

#123765

Purpose

Encodes msGFP as a Type 3a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

msfGFP

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK2_015

Plasmid

#123707

Purpose

Encodes pSynTF (miniCMV core) as a Type 2 part to be used in the MTK sytem

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pSynTF

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3_004

Plasmid

#123720

Purpose

Encodes hph (hygromycin B phosphotransferase) as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Hygro Resistance

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3_005

Plasmid

#123721

Purpose

Encodes ble (Zeocin resistance) as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Zeocin Resistance

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK2_026

Plasmid

#123718

Purpose

Encodes crippled pSFFV as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pSFFVc

Expression

Mammalian

Mutation

Crippled Kozac sequence

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pSKB3-SaGGR-I206F-L377H (JBEI-7202)

Plasmid

#110152

Purpose

pSKB3 with TEV-cleavable, N-terminal His-tagged SaGGR-I206F-L377H mutant gene

Depositor

Jay Keasling

Article

Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

Insert

GGR

Tags

TEV-cleavable His-tag

Expression

Bacterial

Mutation

SaGGR-I206F-L377H mutant gene

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM865-5p

Plasmid

#84186

Purpose

target miRNA865-5p for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM865-5p

Expression

Plant

Promoter

2x35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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MTK1_018

Plasmid

#123681

Purpose

Encodes ConL8 as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L8

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM863-3p

Plasmid

#84182

Purpose

target miRNA863-3p for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM863-3p

Expression

Plant

Promoter

2x35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM863-5p

Plasmid

#84183

Purpose

target miRNA863-5p for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM863-5p

Expression

Plant

Promoter

2x35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK5_011

Plasmid

#123855

Purpose

Encodes ConR8 as a Type 5 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector R8

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK2_014

Plasmid

#123706

Purpose

Encodes pUAS (miniCMV core) as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pUAS (mini CMV)

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK4_004

Plasmid

#123813

Purpose

Encodes spacer sequence as a Type 4 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Spacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK2_018

Plasmid

#123710

Purpose

Encodes truncated pCMV as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pCMV-C

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS-TAL-NC2

Plasmid

#43856

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

TALEN-FokI nuclease backbone

Tags

Flag and FokI nuclease

Expression

Mammalian

Promoter

CAG

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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