We narrowed to 5,559 results for: Chia

1. pBEL2665

   Plasmid

   #195634

   Purpose

   Complementation of MlaA(delta244-251)

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

   Insert

   MlaA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   MlaA(delta244-251)

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBEL2521

   Plasmid

   #195643

   Purpose

   Complementation of MlaC(A34R)

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

   Insert

   MlaC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   MlaC(A34R)

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBEL2512

   Plasmid

   #195626

   Purpose

   Complementation of MlaA(delta238-251)

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

   Insert

   MlaA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   MlaA(delta238-251)

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBEL2513

   Plasmid

   #195627

   Purpose

   Complementation of MlaA(delta227-251)

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

   Insert

   MlaA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   MlaA(delta227-251)

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBEL2639

   Plasmid

   #195628

   Purpose

   Complementation of MlaA(I241N)

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

   Insert

   MlaA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   MlaA(I241N)

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBEL1160

   Plasmid

   #196025

   Purpose

   Expression/purification of 6xHis-TEV-MlaD

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

   Insert

   MlaD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBEL1161

   Plasmid

   #196026

   Purpose

   Expression/purification of 6xHis-TEV-MlaDdelta141-183

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

   Insert

   MlaD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   6xHis-TEV-MlaDdelta141-183

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBEL1195

   Plasmid

   #196027

   Purpose

   Complementation of MlaF-MlaE-MlaD-MlaC-MlaB

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

   Insert

   MlaFEDCB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ptRNAfMet-A37U

   Plasmid

   #196659

   Purpose

   Plasmid for overexpression of mutated initiator tRNA (A37U)

   Depositor

   Markus Jeschek

   Article

   Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

   Insert

   tRNAfMet-A37U expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A37U in tRNAfMet

   Promoter

   metY native promoter

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ptRNAfMet-A37G

    Plasmid

    #196658

    Purpose

    Plasmid for overexpression of mutated initiator tRNA (A37G)

    Depositor

    Markus Jeschek

    Article

    Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

    Insert

    tRNAfMet-A37G expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A37G in tRNAfMet

    Promoter

    metY native promoter

    Available since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRnc2-gGFP

    Plasmid

    #189563

    Purpose

    Expresses E. Coli RNase III under control of pBAD promoter with native 5' untranslated region and constitutively expresses gUTR-129-GFP

    Depositor

    Marc Ostermeier

    Article

    Weeks et al Mol Biol Evol. 2023 Feb 27:msad047. doi:

    Inserts

    RNase III
    gUTR-129-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Maintains 5' UTR of rnc

    Promoter

    J23119 and pBAD

    Available since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSLS.539

    Plasmid

    #184958

    Purpose

    Test effect of a1/a2 length on editing fabH using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 12
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    fabH donor G954A

    Promoter

    T7/lac

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pXD70LacZ4-PdegV-LacZ

    Plasmid

    #191620

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), PdegV promoter-lacZ, E. lenta constitutive promoter

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pXD70LacZ8.1-Pcadh-LacZ

    Plasmid

    #191624

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), Pcadh catechin dehydroxylase promoter-lacZ

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pXD70LacZ9-Phcdh-LacZ

    Plasmid

    #191625

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), Phcdh hydrocaffeic acid dehydroxylase promoter-lacZ

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSCL.103

    Plasmid

    #184988

    Purpose

    Express Cas9-P2A-Eco1RT

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Cas9-P2A-Eco1RT
    

    Use

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSCL.031

    Plasmid

    #184968

    Purpose

    Test effect of extending -Eco2 a1/a2 in yeast

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco2: RT and ncRNA(extended), a1/a2 length: 29
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    human codon optimized RT, a1/a2 length extended t…

    Promoter

    Gal7

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSCL.027

    Plasmid

    #184964

    Purpose

    Express -Eco1 RT and ncRNA in yeast

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: RT and ncRNA(wt), a1/a2 length: 12
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal7

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSCL.72

    Plasmid

    #184984

    Purpose

    Express Cas9/-Eco1 RT fusion, using linker1

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Cas9-fusion_linker1-Eco1RT
    

    Use

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSCL.95

    Plasmid

    #184986

    Purpose

    Express Cas9/-Eco1 RT fusion, using linker2

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Cas9-fusion_linker2-Eco1RT
    

    Use

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSCL.102

    Plasmid

    #184987

    Purpose

    Express -Eco1 RT-P2A-Cas9

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1RT-P2A-Cas9
    

    Use

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSLS.544

    Plasmid

    #184961

    Purpose

    Test effect of a1/a2 length on editing murF using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 22
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    murF donor G1359A, a1/a2 length extended to 22 bp

    Promoter

    T7/lac

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSCL.94

    Plasmid

    #184985

    Purpose

    Express -Eco1 RT/Cas9 fusion, using linker2

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1RT-fusion_linker2-Cas9
    

    Use

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSCL.006

    Plasmid

    #184970

    Purpose

    Express -Eco1 RT in yeast

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Integrating inducible cassette: Eco1 RT
    

    Use

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSLS.543

    Plasmid

    #184960

    Purpose

    Test effect of a1/a2 length on editing murF using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 12
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    murF donor G1359A

    Promoter

    T7/lac

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSCL.71

    Plasmid

    #184983

    Purpose

    Express -Eco1 RT/Cas9 fusion, using linker1

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1RT-fusion_linker1-Cas9
    

    Use

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSLS.545

    Plasmid

    #184962

    Purpose

    Test effect of a1/a2 length on editing priB using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 12
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    priB donor G1069A

    Promoter

    T7/lac

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBEL2200

    Plasmid

    #170407

    Purpose

    Genetic complementation plasmid of LetA-[LetB PLL6->3] mutant

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

    Insert

    LetAB (letB, letA E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(Δ702-716, replace with 347-360)

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBEL2201

    Plasmid

    #170408

    Purpose

    Genetic complementation plasmid of LetA-[LetB PLL6->5] mutant

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

    Insert

    LetAB (letB, letA E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(Δ702-716, replace with 580-600)

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBEL2254

    Plasmid

    #170409

    Purpose

    Genetic complementation plasmid of LetA-[LetB PLL6->2] mutant

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

    Insert

    LetAB (letB, letA E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(Δ700-717, replace with 225-249)

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBEL2255

    Plasmid

    #170410

    Purpose

    Genetic complementation plasmid of LetA-[LetB PLL6->4] mutant

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

    Insert

    LetAB (letB, letA E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(Δ700-716, replace with 445-479)

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBEL2256

    Plasmid

    #170411

    Purpose

    Genetic complementation plasmid of LetA-[LetB PLL6->7] mutant

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

    Insert

    LetAB (letB, letA E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(Δ700-717, replace with 812-836)

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBEL2116

    Plasmid

    #170393

    Purpose

    Plasmid for genetic complementation of LetAB with ΔRing 1 LetB mutant

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

    Insert

    LetAB (letB, letA E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(Δ48-159)

    Promoter

    T7

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBEL2117

    Plasmid

    #170394

    Purpose

    Plasmid for genetic complementation of LetAB with ΔRing 2 LetB mutant

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

    Insert

    LetAB (letB, letA E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(Δ160-279)

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBEL2118

    Plasmid

    #170395

    Purpose

    Plasmid for genetic complementation of LetAB with ΔRing 3 LetB mutant

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

    Insert

    LetAB (letB, letA E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(Δ280-391)

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBEL2119

    Plasmid

    #170396

    Purpose

    Plasmid for genetic complementation of LetAB with ΔRing 4 LetB mutant

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

    Insert

    LetAB (letB, letA E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(Δ392-515)

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBEL2120

    Plasmid

    #170397

    Purpose

    Plasmid for genetic complementation of LetAB with ΔRing 5 LetB mutant

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

    Insert

    LetAB (letB, letA E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(Δ516-633)

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBEL2121

    Plasmid

    #170398

    Purpose

    Plasmid for genetic complementation of LetAB with ΔRing 6 LetB mutant

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

    Insert

    LetAB (letB, letA E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(Δ634-746)

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBEL2122

    Plasmid

    #170399

    Purpose

    Plasmid for genetic complementation of LetAB with ΔRing 7 LetB mutant

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

    Insert

    LetAB (letB, letA E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(Δ747-877)

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBEL2199

    Plasmid

    #170406

    Purpose

    Genetic complementation plasmid of LetA-[LetB PLL6->1] mutant

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

    Insert

    LetAB (letB, letA E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB(Δ702-717, replace with 114-130)

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pBEL2114

    Plasmid

    #175961

    Purpose

    For expression of LetA-[ΔRing6 LetB] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

    Inserts

    LetA
    LetB
    

    Use

    Tags

    8xHis 2xQH and TEV cleavage site on LetA

    Expression

    Bacterial

    Mutation

    Δ634-746

    Promoter

    araBAD

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pTS2341_Tier3(SB)-Puro

    Plasmid

    #169639

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-PuroR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven PuroR expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRPBSA

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pRSF-1b-S19-T48C

    Plasmid

    #128597

    Purpose

    Expresses tagless S19-T48C mutant 30S ribosomal protein in E.coli

    Depositor

    James Williamson

    Article

    Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

    Insert

    30S ribosomal subunit protein S19 (rpsS E.coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T48C

    Promoter

    T7

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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44. pRSF-1b-S15-T79C

    Plasmid

    #133048

    Purpose

    Expresses tagless S15-T79C mutant ribosomal protein in E.coli

    Depositor

    James Williamson

    Article

    Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

    Insert

    S15 E.coli ribosomal protein (rpsO E.coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T79C

    Promoter

    T7

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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45. pRSF-1b-S7-S83C/Δ157-178

    Plasmid

    #128592

    Purpose

    Expresses tagless S7-S83C, Δ157-178 mutant 30S ribosomal protein in E.coli

    Depositor

    James Williamson

    Article

    Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

    Insert

    S7 E.coli ribosomal protein (rpsG E.coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S83C, Δ157-178

    Promoter

    T7

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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46. AraC-pHG165c WT

    Plasmid

    #170114

    Purpose

    Constitutively expressed, wild-type E coli AraC transcription activator; activated by L-Arabinose.

    Depositor

    Liskin Swint-Kruse

    Article

    Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

    Insert

    AraC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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47. pMRBad-C-wtBlc

    Plasmid

    #168257

    Purpose

    C-fragment. This plasmid can be co-transformed with either Plasmid 168259: pET11a-N-wtBlc or Plasmid 168472: pET11a-N-DiB2 to obtain wtBlc‐split or DiB2‐split protein, correspondingly

    Depositor

    Jens Meiler

    Article

    Bozhanova et al FEBS Lett. 2021 Jan;595(2):206-219. doi:

    Insert

    C-fragment of wtBlc
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. pET19b-rDAD2 cPGFP(N180)

    Plasmid

    #166001

    Purpose

    Expression of coding sequence in Escherichia coli

    Depositor

    Claudia Vickers

    Article

    Chesterfield et al ACS Synth Biol. 2020 Aug 21;9(8):2107-21

    Insert

    rDAD2 cPGFP(N180)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LSSmOrange- V49A (please see depositor comments)

    Promoter

    T7 promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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49. pPB-tetpA(R1R4)-iRPT

    Plasmid

    #154882

    Purpose

    Ubiquitously expresses iRFP713, Puromycin resistance gene, and rtTAM2.

    Depositor

    Hideyuki Okano

    Article

    Nemoto et al Cells. 2020 Dec 22;10(1). pii: cells1001

    Inserts

    iRFP713
    Puromycin resistance gene
    rtTAM2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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50. pBEL2082

    Plasmid

    #155162

    Purpose

    Expresses (Strep-MlaF_Δ[248-269])-(6xHis-TEV-MlaB)

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    (MlaFΔ[248-269])-(6xHis-TEV-MlaB)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    deleted amino acids 248-269 in MlaF

    Promoter

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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