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pcDNA3.1(+)/Puro/FA/FFT-LL5BIP

Plasmid

#112830

Purpose

transient/stable overexpression

Depositor

Tomasz Prószyński

Article

Niewiadomski et al Matters v. 2, n. 11 (Nov 17, 2016)

Insert

LL5BIP (2410002F23Rik Mouse)

Tags

2xFlag-TEV

Expression

Mammalian

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pOSIP-KH-RBS2-dCas9

Plasmid

#182740

Purpose

Integrative plasmid at the HK022 site in E. coli carrying dCas9 under the control of a Ptet promoter

Depositor

David Bikard

Article

Rousset et al PLoS Genet. 2018 Nov 7;14(11):e1007749.

Insert

dCas9

Use

CRISPR; Integrative vector

Promoter

Ptet

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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f1H (264)

Plasmid

#24425

Depositor

Steven Rosen

Article

Morimoto-Tomita et al Neoplasia. 2005 Nov . 7(11):1001-10.

Insert

SULF1 (SULF1 Human)

Tags

FLAG, His, and Myc

Expression

Mammalian

Mutation

22aa N-terminal truncation

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
ATF4 13: 5’ATF4.uORF2AUA.GFP

Plasmid

#21860

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

AUG codon of uORF2 was converted to AUA

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pPro7(E)-gfp

Plasmid

#17812

Depositor

Jay Keasling

Article

Lee et al Gene. 2006 Aug 1;377:6-11. doi: 10.1016/

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
CMV-mCherry-3’UTR of β-actin-(F30-2xdBroccoli)6

Plasmid

#190162

Purpose

Expresses 24 Broccoli-tagged beta-actin mRNA in mammalian cells

Depositor

Samie Jaffrey

Article

Li et al Angew Chem Int Ed Engl. 2020 Mar 9;59(11

Insert

mcherry_(3'UTR, No Actin)_24Broccoli

Tags

24×Broccoli

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-N-gamma-tubulin (1-333)

Plasmid

#87858

Purpose

Fluorescent fragment of gamma-tubulin that lacks gamma-tubulin DNA binding domain and NLS

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

gamma-tubulin 1 (TUBG1 Human)

Tags

GFP

Expression

Mammalian

Mutation

Fragment from aa 1 to 333

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pHSG-PCNA1

Plasmid

#66125

Purpose

S. solfataricus PCNA1

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Chembiochem. 2010 Jul 26;11(11):1517-20.

Insert

DNA polymerase sliding clamp 1 (SSO_RS01995 Sulfolobus solfataricus P2)

Use

Cloning vector

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pattB-nsyb-MKIIK11A::nlsLexADBDo

Plasmid

#64726

Purpose

generating transgenic TRIC flies

Depositor

Liqun Luo

Article

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Insert

MKIIK11A::nlsLexADBDo

Expression

Insect

Available Since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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pUASTattB-MKII::nlsLexADBDo

Plasmid

#64718

Purpose

generating transgenic TRIC flies

Depositor

Liqun Luo

Article

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Insert

MKII::nlsLexADBDo

Expression

Insect

Available Since

May 6, 2015

Availability

Academic Institutions and Nonprofits only

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pattB-nsyb-MKII::GAL4DBDo

Plasmid

#64721

Purpose

generating transgenic TRIC flies

Depositor

Liqun Luo

Article

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Insert

MKIIK11A::GAL4DBDo

Expression

Insect

Available Since

May 6, 2015

Availability

Academic Institutions and Nonprofits only

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ACA

Plasmid

#27790

Depositor

Steven Vogel

Article

Koushik et al PLoS One. 2009 Nov 25;4(11):e8031. doi:

Insert

Amber-Cerulean-Amber

Use

Stoichiometry standard

Expression

Mammalian

Available Since

June 23, 2011

Availability

Academic Institutions and Nonprofits only
(attB) tdMCP-tagged BRaf with MS2-circRNA

Plasmid

#226163

Purpose

Used to integrate 3xFlag-tdMCP-BRaf and MS2-circRNA barcodes into RNA-Protein Landing Pad cell lines

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Inserts

3xFlag-tdMCP-BRaf (BRAF Human)
IRES-mCherry-P2A-PuroR
MS2-circRNA barcode

Use

Bxb1 attb, no promoter

Tags

3xFlag-tdMCP

Expression

Mammalian

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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HaloTag-LaminA

Plasmid

#231894

Purpose

Expression of HaloTag-tagged LaminA to visualize the nuclear lamina

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

LaminA (LMNA Human)

Tags

HaloTag

Expression

Mammalian

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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emiRFP670-cGAS

Plasmid

#231896

Purpose

Expression of cyclic GMP/AMP synthase (cGAS), as a nuclear rupture marker

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

cGAS (CGAS Human)

Tags

emiRFP670

Expression

Mammalian

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pJMP3653

Plasmid

#222348

Purpose

Expression vector with PabstBR promoter, kanR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

Depositor

Jason Peters

Article

Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013

Type

Empty backbone

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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plk1 T210D

Plasmid

#132964

Purpose

To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

Depositor

Michael Yaffe

Article

Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

Insert

polo-like kinase 1 (PLK1 Human)

Expression

Mammalian

Mutation

T210D

Available Since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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PGAL

Plasmid

#60337

Purpose

Contains full length native galactose promoter

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Inserts

promoter
yECitrine

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available Since

Nov. 6, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX-GP-2-DNMT3A PWWP

Plasmid

#59696

Purpose

Contains histone modification interacting domain (DNMT3A PWWP)

Depositor

Albert Jeltsch

Article

Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

Insert

DNMT3A PWWP (Dnmt3a Mouse)

Expression

Bacterial

Promoter

tac promoter

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pBabe-GW-HA-RINT1

Plasmid

#39841

Depositor

Lynda Chin

Article

Quayle et al Neuro Oncol. 2012 Nov;14(11):1325-31. do

Insert

RINT1 (RINT1 Human)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Available Since

July 16, 2013

Availability

Academic Institutions and Nonprofits only

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