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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 2,384 results for: LARGE;

Showing: 1941 - 1960 of 2384 results

  4. pET-AtC60αβC20NtL

    Plasmid
    #160876
    Purpose
    Expression of N. tabacum Rubisco large subunit (rbcL) and A. thaliana Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli
    Depositor
    Maureen Hanson
    Article
    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do
    Insert
    rbcL, cpn60α, cpn60β, cpn20
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rbcL: T7, chaperones: T7
    Available since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    160876-plasmid-map-sequence-id-317688

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  9. pET28a-LSSmGFP-TEV-SAV

    Plasmid
    #191200
    Purpose
    Fusion of LSSmGFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification
    Depositor
    Gregory Petsko
    Article
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Insert
    LSSmGFP & Streptavidin fusion
    Use
    Tags
    6xHis and Streptavidin core domain, amino acids 1…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    191200-plasmid-map-sequence-id-376986

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  10. RT-4

    Plasmid
    #220443
    Purpose
    Construct used to generate the PUF3/PUP2/3HA tagging strains. Surrounding the PUP2/3HA/URA3 are large regions of homology to integrate at the 3’ end of the PUF3 gene.
    Depositor
    Marvin Wickens
    Article
    Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do
    Insert
    PUF3 (PUF3 Budding Yeast)
    Use
    Tags
    3xHA
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220443-plasmid-map-sequence-id-436941

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  12. YIP sHRPa-Aga1P

    Plasmid
    #73151
    Purpose
    Large fragment of split HRP fused to the N-terminus of the yeast mating protein Aga1P. When linearized, this vector incorporates into the yeast genome.
    Depositor
    Alice Ting
    Article
    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103
    Insert
    sHRPa-Aga1P
    Use
    Tags
    V5 tag
    Expression
    Yeast
    Mutation
    Promoter
    glyceraldehyde-3-phosphate dehydrogenase GPD prom…
    Available since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73151-plasmid-map-sequence-id-134538

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  13. pACEC3GH (Hyg)

    Plasmid
    #110084
    Purpose
    Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Type
    Empty backbone
    Use
    Tags
    3C cleavage site - GFP - His10 Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    110084-plasmid-map-sequence-id-211189

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  14. CAG sHRPa-FRB-pre mGRASP

    Plasmid
    #73145
    Purpose
    Large fragment of split HRP and FRB fused to the extracellular terminus of the "pre mGRASP" scaffold published by Magee, in which the extracellular domain of NRX is replaced by portions of CD4.
    Depositor
    Alice Ting
    Article
    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103
    Insert
    sHRPa-FRB-pre mGRASP
    Use
    Tags
    Flag tag
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73145-plasmid-map-sequence-id-134517

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  15. pET19b-EcMscL-TEV-mEGFP

    Plasmid
    #165097
    Purpose
    Expresses EcMscLGFP as a fusion protein in E. coli BL21or using in vitro expression.
    Depositor
    Neha Kamat
    Article
    Jacobs et al Proc Natl Acad Sci U S A. 2019 Feb 13. p
    Insert
    E. Coli Mechanosensitive Channel of Large Conductance (mscL E. coli)
    Use
    Synthetic Biology
    Tags
    monomeric Enhanced Green Fluorescent Protein
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    165097-plasmid-map-sequence-id-324525

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  19. pACEC3GH (Apr)

    Plasmid
    #110085
    Purpose
    Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Type
    Empty backbone
    Use
    Tags
    3C cleavage site - GFP - His10 Tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    110085-plasmid-map-sequence-id-211188

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Showing: 1941 - 1960 of 2384 results