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pGEM-HE-bPAC(R278A)-Myc-eYFP

Plasmid

#165488

Purpose

Xenopus oocyte expression of soluble photoactivatable adenylyl cyclase derived from bPAC with reduced dark activity

Depositor

Thomas Oertner

Article

Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

Insert

bPAC(R278A)-Myc-eYFP

Use

Cdna expression, xenopus oocyte

Tags

EYFP and Myc

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_DW164AA + DED/AAA USP7

Plasmid

#131256

Purpose

Mammalian expression of N-terminally Myc-tagged USP7, with mutations in the TRAF-like domain (DW164AA) and UBL2 (DE758AA_D764A)

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Contains point mutations that convert USP7 aspart…

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-H2-Dd (Y84C/C121S/A139C)

Plasmid

#136063

Purpose

Mammalian expression of myc-tagged H2-Dd (Y84C/C121S/A139C mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

H2-Dd (H2-D1 Mouse)

Use

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

Y84C/C121S/A139C

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-H2-Dd-VN (Y84C/C121S/A139C)

Plasmid

#135501

Purpose

Mammalian expression of VN-fused and myc-tagged H2-Dd (Y84C/C121S/A139C mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

H2-Dd (H2-D1 Mouse)

Use

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

Y84C/C121S/A139C

Promoter

CMV

Available since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 _34-66/myc-His

Plasmid

#127495

Purpose

Expresses myc-tagged _34-66 NDUFS2 in mammalian cells

Depositor

Dieter Wolf, Shashi Jain

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 _34-66 (NDUFS2 Human)

Use

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 _34-66

Promoter

CMV

Available since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 E57A/myc-His

Plasmid

#127497

Purpose

Expresses myc-tagged NDUFS2/E57A in mammalian cells

Depositor

Dieter Wolf, Shashi Jain

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 E57A (NDUFS2 Human)

Use

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 E57A

Promoter

CMV

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 T58A/myc-His

Plasmid

#127498

Purpose

Expresses myc-tagged NDUFS2/T58A in mammalian cells

Depositor

Dieter Wolf, Shashi Jain

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 T58A (NDUFS2 Human)

Use

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 T58A

Promoter

CMV

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 H60A/myc-His

Plasmid

#127499

Purpose

Expresses myc-tagged NDUFS2/H60A in mammalian cells

Depositor

Dieter Wolf, Shashi Jain

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 H60A (NDUFS2 Human)

Use

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 H60A

Promoter

CMV

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 W61A/myc-His

Plasmid

#127500

Purpose

Expresses myc-tagged NDUFS2/W61A in mammalian cells

Depositor

Dieter Wolf, Shashi Jain

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 W61A (NDUFS2 Human)

Use

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 W61A

Promoter

CMV

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 K62A/myc-His

Plasmid

#127501

Purpose

Expresses myc-tagged NDUFS2/K62A in mammalian cells

Depositor

Dieter Wolf, Shashi Jain

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 K62A (NDUFS2 Human)

Use

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 K62A

Promoter

CMV

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 P63A/myc-His

Plasmid

#127502

Purpose

Expresses myc-tagged NDUFS2/P63A in mammalian cells

Depositor

Dieter Wolf, Shashi Jain

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 P63A (NDUFS2 Human)

Use

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 P63A

Promoter

CMV

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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IX104: pMVP (L5-L4) myc epitope tag

Plasmid

#121704

Purpose

pMVP L5-L4 entry plasmid, contains myc epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

myc epitope tag (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti C-MYC-DDK Puro PNRC1

Plasmid

#123300

Purpose

Mammalian expression of PNRC1 C-MYC-FLAG

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

PNRC1 (PNRC1 Human)

Use

Lentiviral

Tags

MYC-FLAG

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pRSF_6His-ZmSUMO1a-GG_ZmSCE1d(C94S)-Myc

Plasmid

#91935

Purpose

Bacterial expression of maize SUMO1a and inactive SCE1d

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

6His-ZmSUMO1a-GG
ZmSCE1d(C94S)-Myc

Use

Tags

6His and Myc

Expression

Bacterial

Mutation

C94S active site mutation and Truncation of ZmSUM…

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRSF_6His-ZmSUMO1a-GG_ZmSCE1b(C94S)-Myc

Plasmid

#91933

Purpose

Bacterial expression of maize SUMO1a and inactive SCE1b

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

6His-ZmSUMO1a-GG
ZmSCE1b(C94S)-Myc

Use

Tags

6His and Myc

Expression

Bacterial

Mutation

C94S active site mutation and Truncation of ZmSUM…

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pQCXIP-Laforin Myc (C266S,C278S)

Plasmid

#84657

Purpose

mammalian expression and/or retrovirus preparation

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-myc-mCE(211-597)

Plasmid

#82462

Purpose

Expression of myc epitope and C-terminal domain of murine mRNA Capping Enzyme in mammalian cells

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

mCE(211-597) (Rngtt Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO myc Separase delta 55

Plasmid

#59824

Purpose

Allows the integration of myc Separase delta 55 in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

Insert

Separase (ESPL1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

delta 55

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Rem2 RNAiR2 short

Plasmid

#51596

Purpose

expresses RNAiR myc-tagged rat Rem2 (short version, missing 69 AA at N terminus)

Depositor

Suzanne Paradis

Article

Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

Insert

Rem2 (Rem2 Rat)

Use

Tags

myc

Expression

Mammalian

Mutation

silent mutations at shRNA 1 and 2 recognition sit…

Promoter

CMV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-myc-Rem2 R236A/L263A

Plasmid

#51589

Purpose

expresses RNAiR myc-tagged Rem2 non-Ca channel interacting

Depositor

Suzanne Paradis

Article

Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

Insert

Rem2 (Rem2 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

R236A and L263A; silent mutations at shRNA 1 and …

Promoter

CMV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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