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pJW4.21

Plasmid

#62875

Purpose

Bacterial expression of MBP-MEG-1

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

MBP-MEG-1

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pFAST-BAC-Cdc20wt (2193)

Plasmid

#39850

Depositor

Jonathon Pines

Article

Nilsson et al Nat Cell Biol. 2008 Dec . 10(12):1411-20

Insert

Cdc20 (CDC20 Human)

Use

Baculovirus expression

Tags

His

Expression

Mutation

Promoter

Available since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only
POM43-mCitrine

Plasmid

#69855

Purpose

N-terminal tagging with mCitrine using LEU2 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mCitrine minus start and stop codon

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 16, 2018

Availability

Academic Institutions and Nonprofits only

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pBS YBX1

Plasmid

#19217

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

YBX1 (YBX1 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
PI(LM)-Rac1(WT)dc1.FLARE

Plasmid

#87590

Purpose

PI(LM): Photo-inhibitable/lit mutant I510E/I539E dc1.FLARE: built based on GTPase FLARE dual chain sensor

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
PBD
Rac1

Use

Tags

YPet and dTurq

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

CMV

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET15b-At 14-3-3 phi

Plasmid

#44939

Depositor

Robert Ferl

Article

Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

Insert

14-3-3 phi (GF14 PHI Mustard Weed)

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
pAB029f

Plasmid

#79220

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ lambda cI-SH2ABL1; PlacIq lacI

Use

Tags

Expression

Bacterial

Mutation

Promoter

PlacZ; PlacIq

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRPB1-CTD14

Plasmid

#112822

Purpose

pSMF2 with 14 CTD repeats

Depositor

Stephen Fuchs

Article

Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

Insert

RPB1 (RPO21 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

CTD repeats have identical sequence, deleted repe…

Promoter

Native promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB031a

Plasmid

#79221

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ HA4-Zif268; PlacIq lacI

Use

Tags

Expression

Bacterial

Mutation

Promoter

PlacZ; PlacIq

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b-At 14-3-3 lambda

Plasmid

#44942

Depositor

Robert Ferl

Article

Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

Insert

14-3-3 lambda (GRF6 Mustard Weed)

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
pET28-his-3C_Clu3B6p

Plasmid

#100093

Purpose

expresses easily crystallizable engineered 14-3-3 sigma variant fused to the HSPB6 phosphorylatable peptide

Depositor

Nikolai Sluchanko

Article

Sluchanko et al Sci Rep. 2017 Sep 20;7(1):12014. doi: 10

Insert

14-3-3 sigma human (SFN Human)

Use

Tags

HSPB6 peptide and His6

Expression

Bacterial

Mutation

75-77 residues changed to AAA to facilitate cryst…

Promoter

T7

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pAB043c

Plasmid

#79223

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ lambda cI-p38-alpha; PlacIq lacI

Use

Tags

Expression

Bacterial

Mutation

Promoter

PlacZ; PlacIq

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRPB1-CTD8

Plasmid

#112820

Purpose

pSMF2 with 8 CTD repeats

Depositor

Stephen Fuchs

Article

Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

Insert

RPB1 (RPO21 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

CTD repeats have identical sequence, deleted repe…

Promoter

Native promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRPB1-CTD10

Plasmid

#112821

Purpose

pSMF2 with 10 CTD repeats

Depositor

Stephen Fuchs

Article

Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

Insert

RPB1 (RPO21 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

CTD repeats have identical sequence, deleted repe…

Promoter

Native promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB042b

Plasmid

#79193

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Plac62 gIII, luxAB; Ptet rpoZ-MBPoff7

Use

Tags

Expression

Bacterial

Mutation

Promoter

Plac62; Ptet

Available since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB042d

Plasmid

#79194

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Plac62 gIII, luxAB; Ptet rpoZ-p38_2_3

Use

Tags

Expression

Bacterial

Mutation

Promoter

Plac62; Ptet

Available since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PI(DM)-Rac1(WT)dc1.FLARE

Plasmid

#87589

Purpose

PI(DM): Photo-inhibitable/dark mutant C450A; dc1.FLARE: built based on GTPase FLARE dual chain sensor

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
PBD
Rac1

Use

Tags

YPet and dTurq

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

CMV

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pJW4.17

Plasmid

#62872

Purpose

Bacterial expression of MBP-MBK-2 K196R

Depositor

Geraldine Seydoux

Article

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

MBP-MBK-2 K196R

Use

Tags

MBP

Expression

Bacterial

Mutation

K196R

Promoter

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only
pAB043a

Plasmid

#79222

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ lambda cI-MBP; PlacIq lacI

Use

Tags

Expression

Bacterial

Mutation

Promoter

PlacZ; PlacIq

Available since

April 15, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCVD447

Plasmid

#78569

Purpose

E. coli E2348/69 LEE region (Type 3 Secretion System) cloned into pBluescriptKS.

Depositor

James Kaper

Article

Jarvis et al Proc Natl Acad Sci U S A. 1995 Aug 15;92

Insert

lee operon

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only