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pLL7.0: Venus-iLID-CAAX (from KRas4B)

Plasmid

#60411

Purpose

Mammalian Expression of Venus-iLID-CAAX

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

Venus-iLID-CAAX

Use

Cre/Lox and Lentiviral

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pJZC43

Plasmid

#66565

Purpose

sgRNA + 2XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

sgRNA + 2XPP7
PCP-VP64 IRES mCherry

Expression

Mammalian

Promoter

CMV and U6

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pT2/shp53/GFP4

Plasmid

#20208

Depositor

John Ohlfest

Article

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

shp53/GFP4

Use

Transposon

Available Since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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Cas9-GFP_sg_mAMPKa1

Plasmid

#79004

Purpose

Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 1.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

Prkaa1 (Prkaa1 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3 MYC-Sumo3 WT

Plasmid

#48964

Purpose

Expresses MYC-Sumo3 Wild Type in mammalian cells

Depositor

Guy Salvesen

Article

Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

Insert

SUMO3 (SUMO3 Human)

Tags

MYC (EQKLISEED)

Expression

Mammalian

Promoter

T7

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only
pEQ176

Plasmid

#83943

Purpose

express beta-galactosidase in eukaryotic cells

Depositor

Adam Geballe

Article

Schleiss et al J Virol. 1991 Dec;65(12):6782-9.

Insert

CMV enhancer/promoter

Tags

beta-galactosidase

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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mNeongreen-PXN

Plasmid

#129604

Purpose

In vivo visualization of focal adhesions (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Paxillin

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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dxCas(3.7)-VPR

Plasmid

#108383

Purpose

Mammalian expression vector for xCas9 3.7 with VP64-p65-Rta (VPR) fused to its C-terminus

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

dxCas(3.7)-VPR

Tags

NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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CFP-Parkin

Plasmid

#47560

Purpose

CFP tagged Parkin for colocalization study

Depositor

Richard Youle

Article

Narendra et al J Cell Biol. 2008 Dec 1. 183(5):795-803.

Insert

Parkin (PRKN Human)

Tags

CFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only

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EF.hICN1.CMV.GFP

Plasmid

#17623

Purpose

Depositor

Linzhao Cheng

Article

Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

Insert

ICN1 (NOTCH1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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