Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 155,989 results for: ins

Showing: 2941 - 2970 of 155989 results

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pFB1.HMBP.PrS.EZH2-mt1

Plasmid

#220239

Purpose

Expression of an EZH2 mutant in insect cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 mt 1 (EZH2 Human)

Tags

6x His, MBP

Expression

Insect

Mutation

R27A, R31A, F165A

Promoter

Polyhedrin

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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NEIL3-mNeonGreen

Plasmid

#215144

Purpose

Expresses NEIL3-mNeonGreen in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

NEIL3 (NEIL3 Human)

Use

Retroviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-CIM1mut-F961A_S

Plasmid

#147514

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_635-1384-CIM1-F961A

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_635-1384-CIM1-F961A (gw Human, Nematode)

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-M2WA-CIM1-2mut-siRNAres_S

Plasmid

#147517

Purpose

Insect Expression of HsTNRC6A-M2WA-CIM1-2mut-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A-M2WA-CIM1-2mut-siRNAres (TNRC6A Human)

Expression

Insect

Mutation

one non silent mutation K1578E compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_205-830-dsRNAres_S

Plasmid

#147513

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_205-830-dsRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_205-830-dsRNAres (gw Fly, Nematode)

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-CIM1-2mut-siRNAres_R

Plasmid

#147412

Purpose

Insect Expression of HsTNRC6A-CIM1-2mut-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A-CIM1-2mut-siRNAres (TNRC6A Human)

Expression

Insect

Mutation

one non silent mutation K1578E compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-M2WA_R

Plasmid

#147387

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_635-1384-M2WA

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_635-1384-M2WA (gw Fly, Nematode)

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut_R

Plasmid

#147388

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut (gw Fly, Nematode)

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-P-GLmut_R

Plasmid

#147390

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_635-1384-P-GLmut

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_635-1384-P-GLmut (gw Human, Nematode)

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-P-GLmut-F961A_R

Plasmid

#147391

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_635-1384-P-GLmut-F961A

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_635-1384-P-GLmut-F961A (gw Fly)

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-CIM1-2mut-F1359A-siRNAres_R

Plasmid

#147411

Purpose

Insect Expression of HsTNRC6A-CIM1-2mut-F1359A-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A-CIM1-2mut-F1359A-siRNAres (TNRC6A Human)

Expression

Insect

Mutation

one non silent mutation K1578E compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_1-634-dsRNAres_R

Plasmid

#147382

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_1-634-dsRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_1-634-dsRNAres (gw Fly, Nematode)

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_205-490-dsRNAres_R

Plasmid

#147383

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_205-490-dsRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_205-490-dsRNAres (gw Fly, Nematode)

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-CIM1-mut_R

Plasmid

#147385

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_635-1384-CIM1-mut

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_635-1384-CIM1-mut (gw Fly, Nematode)

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-del183-258-siRNAres-V5His6_L

Plasmid

#146862

Purpose

Insect Expression of DmPABPC1_del183-258-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmPABPC1_del183-258-siRNAres (pAbp Fly)

Expression

Insect

Mutation

one non silent mutation G26S, and seven silent mu…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-del288-359-siRNAres-V5His6_L

Plasmid

#146863

Purpose

Insect Expression of DmPABPC1_del288-359-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmPABPC1_del288-359-siRNAres (pAbp Fly)

Expression

Insect

Mutation

one non silent mutation G26S, and six silent muta…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-DmPABPC1-del364-550-siRNAres-V5His6_L

Plasmid

#146864

Purpose

Insect Expression of DmPABPC1_del364-550-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmPABPC1_del364-550-siRNAres (pAbp Fly)

Expression

Insect

Mutation

one non silent mutation G26S, and five silent mut…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-pSMANTIS_SMAD-FOS_Mutant

Plasmid

#194188

Purpose

Includes the promoter (1kb) of SMTS with mutated SMAD/FOS binding site

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

Insert

SMANTIS promoter (1kb) (SMANTIS Human)

Use

Luciferase

Mutation

mutated SMAD/FOS Site, Region 3-15nt of 1000nt

Promoter

SMANTIS promoter (1kb), mutated

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-pSMANTIS_STAT1-2_Mutant

Plasmid

#194189

Purpose

Includes the promoter (1kb) of SMTS with mutated STAT1/2 binding site

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

Insert

SMANTIS promoter (1kb) (SMANTIS Human)

Use

Luciferase

Mutation

mutated STAT1/2 site, Region 653-673nt of 1000nt

Promoter

SMANTIS promoter (1kb), mutated

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Granulin 7-no linker

Plasmid

#176931

Purpose

express Granulin 7 without linker 8 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 7 (GRN Human)

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Granulin 7+linker8

Plasmid

#176930

Purpose

express Granulin 7 with linker 8 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 7+linker8 (GRN Human)

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Granulin 6-no linker

Plasmid

#176929

Purpose

express Granulin 6 without linker 7 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 6 (GRN Human)

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Granulin 6+linker7

Plasmid

#176928

Purpose

express Granulin 6 with linker 7 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 6+linker7 (GRN Human)

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)_Granulin 5-no linker

Plasmid

#176927

Purpose

express Granulin 5 without linker 6 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 5 (GRN Human)

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Granulin 5+linker6

Plasmid

#176926

Purpose

express Granulin 5 with linker 6 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 5+linker6 (GRN Human)

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)_Granulin 3-no linker

Plasmid

#176923

Purpose

express Granulin 3 without linker 4 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 3 (GRN Human)

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)_Granulin 3+linker4

Plasmid

#176922

Purpose

express Granulin 3 with linker 4 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 3+linker4 (GRN Human)

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)_Granulin 1-no linker

Plasmid

#176919

Purpose

express Granulin 1 without linker 2 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 1 (GRN Human)

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223 ATG3 allKR

Plasmid

#129295

Purpose

Gateway entry clone encoding human ATG3 with all 22 lysine residues mutated to arginine

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Use

Gateway Cloning

Mutation

changed all 22 Lysine residues to Arginine

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-ATG3 allKR

Plasmid

#129298

Purpose

Expresses pCMV 3xFLAG-ATG3 allKR (all lysines mutated to arginine) in mammalian cells

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

changed all 22 Lysine residues to Arginine

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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