We narrowed to 4,209 results for: abo
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Plasmid#58325PurposeDestination vector for inserting ubi-CadTS to Drosophila melanogaster genomeDepositorInsertE-cadherin tension sensor
UseTagsExpressionInsectMutationPromoterpoly-ubiquitinAvailable sinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKF-D2irTNP2AG-T2APuroR-5kwh
Plasmid#128255PurposeMammalian linearizer gene circuit based on negative feedback in a Flp-In expression system controlling the PuroR gene.DepositorInserthTetR::P2A::EGFP::T2A::PuroR
UseSynthetic Biology; Flp-in expression vectorTagsExpressionMammalianMutationPromoterCMV-D2ir promoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-T2-NLuc
Plasmid#135938PurposeExpresses NanoLuciferase targeted to the mitochondira in mammalian cells.DepositorInsertNanoLuciferase
UseTagsT2 mitochondrial localization sequenceExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
psbA2(noATbox)-PHLS (c)
Plasmid#52308PurposeReplaces the psbA2 gene in Synechocystis with the PHLS geneDepositorInsertsbeta-phellandrene synthase
Chloramphenicol resistance
UseSynthetic BiologyTagsExpressionBacterialMutationCAAATACA box in the psbA2 promoter to ggcgcgccPromoterpsbA2Available sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
cpc-PHLS (g)
Plasmid#52311PurposeReplaces the cpc operon in Synechocystis with the PHLS geneDepositorInsertsbeta-phellandrene synthase
Chloramphenicol resistance
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterSynechocystis endogenous cpc-operon promoterAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pB6-Csrp2-T2A-sfGFP-iCre-ERT2-FNF-DTA
Plasmid#140270PurposeB6J Csrp2-T2A-sfGFP-iCre-ERT2 allele targeting vector, low copy numberDepositorInsertCsrp2 (Csrp2 Mouse)
UseCre/Lox and Mouse TargetingTagsC-terminal fusion of T2A-sfGFP-iCre-ERT2ExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMTG03-AmpFRT
Plasmid#128272PurposeTIP-LITer1.0 gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-TIP---CMV-TetOx2-GFPDepositorInsertsTetR-LOV-TIP
EGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMTG05-Amp-FRT
Plasmid#128274PurposeTIP-LITer2.0 gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-TIP-P2A-GFPDepositorInsertsTetR-LOV-TIP
EGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMTG04-AmpFRT
Plasmid#128273PurposeDeg-LITer1.0 gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-RRRG---CMV-TetOx2-GFPDepositorInsertsTetR-LOV-RRRG
EGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUWR-Cad-mTFP
Plasmid#58327PurposeDestination vector for inserting ubi-Cad-mTFP to Drosophila melanogaster genomeDepositorInsertCad-mTFP
UseTagsExpressionInsectMutationPromoterpoly-ubiquitinAvailable sinceAug. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
psbA2-Ptrc-T7-g10-PHLS (e)
Plasmid#52310PurposeReplaces the psbA2 gene in Synechocystis with the Ptrc promoter-T7-g10-PHLS geneDepositorInsertsbeta-phellandrene synthase
Chloramphenicol resistance
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtrc-T7Available sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLVTHM-shREV3-4
Plasmid#38033DepositorUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
psbA2-PHLS (b)
Plasmid#52307PurposeReplaces the psbA2 gene in Synechocystis with the PHLS geneDepositorInsertsbeta-phellandrene synthase
Chloramphenicol resistance
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpsbA2Available sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
psbA2-Ptrc-PHLS (d)
Plasmid#52309PurposeReplaces the psbA2 gene in Synechocystis with the Ptrc promoter - PHLS geneDepositorInsertsbeta-phellandrene synthase
Chloramphenicol resistance
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtrcAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmEos3.1-N1 GBP
Plasmid#220492PurposeExpression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos3.1 to track GFP proteins of interest to perform Fluorescent intrabody Localization MicroscopyDepositorInsertGFP Binding Protein tagged with mEos3.1
UseTagsmEos3.1ExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
Ydf NanoLuc
Plasmid#225656PurposeReporter plasmid expressing NanoLuciferase under the control of the promoter PydfJ115, cognate to YdfI. Responds to NarL/NarX chimeras from this study.DepositorInsertNanoLuciferase
UseCell free expressionTagsMycExpressionBacterialMutationPromoterPydfJ115Available sinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NarX Myc
Plasmid#225651PurposeExpresses WT NarX capable of sensing nitrate. For use with NarL-YdfI and reporter plasmids from this study.DepositorInsertNarX (narX E. coli)
UseCell free expressionTagsMycExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-hU6-2O-crScaffold_SV40-BFP
Plasmid#224861PurposecrRNA scaffold for RfxCas13d expressed from hU6-2xTetO promoter and reporter BFP protein expressed from SV40 promoterDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhU6-2xTetOAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-hU6-2O-crScaffold_EF1a-BFP
Plasmid#224862PurposecrRNA scaffold for RfxCas13d expressed from hU6-2xTetO promoter and reporter BFP protein expressed from EF1a promoterDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhU6-2xTetOAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
LPUtopia-8
Plasmid#224781PurposeLanding Pad donor vector for AAVS1 site integration in human and VeroE6 cellsDepositorInsertspuromycin N-acetyltransferase
enhanced Green Fluorescence Protein
Red Fluorescence Protein
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-ODN-hsRPL3-CterTwStrep-ΔPAM62&56
Plasmid#208645PurposeRemove PAM region in HomoArms by introduce SNP rs7495557 and rs760194863, to prevent cleavage after success edit. Digest with nt.BspQI and nb.BsrDI to get ssDNA.DepositorInsertTwin Strep tag with RPL3 genome HomoArm (RPL3 Human, Synthetic)
UseCRISPR; Ssdna donnor preparation for knock-in of…TagsTwin StrepExpressionMutationTwo SNP (rs749555789, rs760194863) were introduce…PromoterAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G121A wgMDH
Plasmid#204252PurposeBacterial expression of G121A wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationG121APromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 Q58L wgMDH
Plasmid#204246PurposeBacterial expression of Q58L wgMDH with IPTG induction - Subunit interface mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationQ58LPromoterT5Available sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R196A wgMDH
Plasmid#204271PurposeBacterial expression of R196A wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationR196APromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R196E wgMDH
Plasmid#204272PurposeBacterial expression of R196E wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationR196EPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 P123A wgMDH
Plasmid#204254PurposeBacterial expression of P123A wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationP123APromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R130Q wgMDH
Plasmid#204268PurposeBacterial expression of R130Q wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationR130QPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G127F wgMDH
Plasmid#204264PurposeBacterial expression of G127F wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationG127FPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 P126R wgMDH
Plasmid#204261PurposeBacterial expression of P126R wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationP126RPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G127A wgMDH
Plasmid#204263PurposeBacterial expression of G127A wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationG127APromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 M66L wgMDH
Plasmid#204247PurposeBacterial expression of M66L wgMDH with IPTG induction - Subunit interface mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationM66LPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G127P wgMDH
Plasmid#204265PurposeBacterial expression of G127P wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationG127PPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 G121F wgMDH
Plasmid#204253PurposeBacterial expression of G121F wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationG121FPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 M91Q wgMDH
Plasmid#204250PurposeBacterial expression of M91Q wgMDH with IPTG induction - Subunit interface mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationM91QPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 D87L wgMDH
Plasmid#204248PurposeBacterial expression of D87L wgMDH with IPTG induction - Subunit interface mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationD87LPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 P126G wgMDH
Plasmid#204260PurposeBacterial expression of P126G wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationP126GPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 P123G wgMDH
Plasmid#204256PurposeBacterial expression of P123G wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationP123GPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 P123F wgMDH
Plasmid#204255PurposeBacterial expression of P123F wgMDH with IPTG induction - Active site loop mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationP123FPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
PX458_3XHA_dFnCas9_KRAB_T2A_eGFP
Plasmid#200389Purpose3XHA tagged dead Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expressionDepositorInsertPX458_3XHA_dFnCas9_KRAB_eGFP
UseCRISPRTags3xHA-NLS and SV40NLS-KRAB-T2A-EGFPExpressionMammalianMutationPromoterCBHAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB3479
Plasmid#190417PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XAO-2XPO::TetR-NLS-19St_proT::GFPint-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_6XAO-2XPO::TetR-NLS-19St_proT::GFPint-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only