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pTwist-SARS-CoV-2 Δ18 B.1.1.7

Plasmid

#169459

Purpose

Encodes SARS-CoV-2 B.1.1.7 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Publication

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 B.1.1.7 Spike truncated to remove 18 amino acids (S )

Expression

Mammalian

Available since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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pKMD111e - intein-Cas9(S219-G521R)

Plasmid

#64192

Purpose

Expresses intein-Cas9(S219-G521R) in mammalian cells

Depositor

David Liu

Publication

Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mammalian codon-optimized S. pyogenes intein-Cas9(S219-G521R) - NLS - 3x FLAG

Use

CRISPR

Tags

NLS - 3x FLAG (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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SpyTag003-MBP

Plasmid

#133450

Purpose

Expresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasm

Depositor

Mark Howarth

Publication

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-MBP

Tags

His6 and Thrombin cleavage site

Expression

Bacterial

Mutation

Peptide tag added to N-terminus of MBP via a GSGE…

Promoter

T7

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pEGFP-TYR

Plasmid

#32781

Depositor

Ruth Halaban

Publication

Halaban et al Proc Natl Acad Sci U S A. 2000 May 23;97

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Tyrosinase (TYR Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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PIK3CA - Exon9 E545K

Plasmid

#16642

Depositor

Bert Vogelstein

Publication

Samuels et al Science. 2004 Apr 23. 304(5670):554.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PIK3CA E545K (PIK3CA Human)

Tags

Flag

Expression

Mammalian

Mutation

E545K; see note below

Available since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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R620-M15-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 D614G

Plasmid

#170201

Purpose

Mammalian expression of SARS-CoV-2 soluble spike trimer protein (D614G mutant)

Depositor

Dominic Esposito

Publication

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SARS-CoV-2 S (spike) D614G (S SARS-CoV-2)

Tags

His6

Expression

Mammalian

Mutation

D614G

Promoter

CMV51

Available since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

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AY27_pU6.gRNA.CLYBL

Plasmid

#199238

Purpose

Expression construct encoding a S. pyogenes guide RNA targeting the human CLYBL safe harbor locus

Depositor

Manuel Goncalves

Publication

Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

Insert

S. pyogenes gRNA spacer

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

RNA polymerase III promoter for human U6 snRNA

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCas9-sgAAVS1-2

Plasmid

#129727

Purpose

Expressing SpCas9 and sgAAVS1-2

Depositor

Elina Ikonen

Publication

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SpCas9 and sgAAVS1-2

Use

CRISPR

Tags

T2A-mCherry-P2A-Puro

Expression

Mammalian

Promoter

hU6

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Kras-Src FRET biosensor

Plasmid

#78302

Purpose

FRET biosensor. To monitor Src kinase activity in mammalian cells by FRET

Depositor

Yingxiao Wang

Publication

Ouyang et al Proc Natl Acad Sci U S A. 2008 Sep 23;10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Src biosensor

Expression

Mammalian

Promoter

CMV

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pGWB502

Plasmid

#74844

Purpose

Gateway cloning compatible binary vector for expression of gene by CaMV35S promoter.

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLenti-EF-FH-TAZ S89A-ires-blast

Plasmid

#52084

Purpose

Lentiviral expression vector for TAZ S89A mutant with N-terminal FLAG and His tags

Depositor

Yutaka Hata

Publication

Yang et al Mol Cell Biol. 2014 Feb 18.

Insert

TAZ S89A (WWTR1 Human)

Use

Lentiviral

Tags

FLAG and His

Expression

Mammalian

Mutation

S89A

Promoter

EF1a

Available since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pSI-Target-AID-NG

Plasmid

#119861

Purpose

Expresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells.

Depositor

Osamu Nureki

Publication

Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

nSpCas9-NG (D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)

Expression

Mammalian

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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p-EGFP-C1-Flag-mFmr1(wt)

Plasmid

#87929

Purpose

Expresses mouse FMRP-EGFP fusion, which can be phosphorylated on ser-499.

Depositor

Stephanie Ceman

Publication

Stetler et al Hum Mol Genet. 2006 Jan 1;15(1):87-96. E

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Fmr1 (Fmr1 Mouse)

Tags

Flag and eGFP

Expression

Mammalian

Mutation

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAG416GPD-ccdB

Plasmid

#14148

Depositor

Susan Lindquist

Publication

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway Cloning

Expression

Yeast

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pGWB402

Plasmid

#74796

Purpose

Gateway cloning compatible binary vector for expression of gene by CaMV35S promoter.

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pGWB554

Plasmid

#74884

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with mRFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-N-Flag-ASC1

Plasmid

#75134

Purpose

Expresses N-terminal Flag-tagged ASC1 in mammalian cells

Depositor

Bruce Beutler

Publication

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

PYD and CARD domain containing (Pycard Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

F23S (please see depositor comments below)

Promoter

CMV

Available since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pcDNA3-N-Flag-Caspase-1

Plasmid

#75128

Purpose

Expresses N-terminal Flag-tagged Caspase-1 in mammalian cells

Depositor

Bruce Beutler

Publication

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

caspase 1 (Casp1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

P341S in Caspase-1 (please see depositor comments…

Promoter

CMV

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pcDNA3 PM-Ras-LOCKR-S

Plasmid

#216498

Purpose

FRET-based reporter for Ras activity, plasma membrane localized

Depositor

David Baker

Publication

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PM-Ras-LOCKR-S

Expression

Mammalian

Available since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLN377 (FuGW-S(SP1)p-mKate2 )

Plasmid

#105176

Purpose

synthetic promoter SP1 drives mKate2 expression

Depositor

Timothy Lu

Publication

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

S(SP1)p-mKate2

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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