We narrowed to 2,426 results for: 683

1. PINK1 gRNA (BRDN0001147554)

   Plasmid

   #78035

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PINK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PINK1 (PINK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   June 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. PINK1 gRNA (BRDN0001145357)

   Plasmid

   #78036

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PINK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PINK1 (PINK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-UbC-eGFP

   Plasmid

   #62518

   Purpose

   Expresses GFP under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing procedures.

   Depositor

   Pantelis Tsoulfas, Jae Lee

   Article

   Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

   Insert

   eGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available Since

   March 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. G protein alpha-s(alpha3beta5)

   Plasmid

   #55799

   Purpose

   This G protein alpha-s mutant exhibits increased affinity for and decreased ability to be activated by Gs-protein-coupled receptors as well as decreased ability to activate adenylyl cyclase.

   Depositor

   Catherine Berlot

   Article

   Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.

   Insert

   G protein alpha-s with alpha-i2 substitutions in alpha3/beta5 loop (Gnas Rat)
   

   Use

   Tags

   internal EE epitope (residues 189-194 in alpha-s …

   Expression

   Mammalian

   Mutation

   N271K, K274D, R280K, T284D, I285T in alpha-s sequ…

   Promoter

   CMV

   Available Since

   July 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. GNAS_pLX307

   Plasmid

   #98339

   Purpose

   Lentiviral expression of GNAS

   Depositor

   Sefi Rosenbluh, William Hahn

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   GNAS (GNAS Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCV-mouse Ezh2 Y637S-IRES-GFP

   Plasmid

   #91887

   Purpose

   Retroviral expression of mouse Ezh2 with Y637S

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   Ezh2 (Ezh2 Mouse)
   

   Use

   Retroviral

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Mutation

   Y637S

   Promoter

   MMLV gag

   Available Since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. G protein alpha-s(alpha3beta5/G226A)

   Plasmid

   #55798

   Purpose

   In addition to the mutations in alpha-s(alpha3beta5), this dominant negative G protein alpha-s mutant contains the G226A mutation, which impairs activating conformation changes in Switch II.

   Depositor

   Catherine Berlot

   Article

   Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.

   Insert

   alpha-s (alpha3beta5/G226A) (Gnas Rat)
   

   Use

   Tags

   internal EE epitope (residues 189-194 in alpha-s …

   Expression

   Mammalian

   Mutation

   N271K, K274D, R280K, T284D, I285T, G226A in alpha…

   Promoter

   CMV

   Available Since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCV-mouse Ezh2 delta SETdomain-IRES-GFP

   Plasmid

   #91885

   Purpose

   Retroviral expression of mouse Ezh2 with a SET domain deletion

   Depositor

   Martine Roussel

   Article

   Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

   Insert

   Ezh2 (Ezh2 Mouse)
   

   Use

   Retroviral

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Mutation

   SET domain deleted

   Promoter

   MMLV gag

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMMK ENTR 1 CMV mAG h-Geminin

   Plasmid

   #206281

   Purpose

   ENTR Vector 1 for MultiSite Gateway assembly. Encodes mAG-hGeminin under the control of CMV

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   mAG-hGeminin (GMNN Human)
   

   Use

   Multimate/gateway entr 1

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. AOI-WT-Cas9-sg-mouse Ezh2-E10-GFP

    Plasmid

    #91879

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Ezh2 (Ezh2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

11. His-PHD2-181-426

    Plasmid

    #223551

    Purpose

    His-tagged human PHD2 catalytic domain with TEV cleavage site between His and PHD2 for PHD2 purification

    Depositor

    Somshuvra Mukhopadhyay

    Article

    Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

    Insert

    PHD2 (EGLN1 Human)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-180.

    Promoter

    trc

    Available Since

    Sept. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCIneoLuc-TEAD4

    Plasmid

    #128335

    Purpose

    Vector for LUMIER assay

    Depositor

    Yutaka Hata

    Article

    Maruyama et al Mol Cancer Res. 2018 Feb;16(2):197-211.

    Insert

    TEAD4 (TEAD4 Human)
    

    Use

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. PINK1 gRNA (BRDN0001487057)

    Plasmid

    #78037

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PINK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PINK1 (PINK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pD649-HAsp-DLL3-COMP5AP-AviTag-9xHis

    Plasmid

    #157437

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    DLL3 (DLL3 Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. LentiCRISPRv2-sgCD20

    Plasmid

    #209749

    Purpose

    Lentiviral transfer plasmid to express Cas9 and a gRNA targeting the human CD20 gene, MS4A1.

    Depositor

    Andrei Thomas-Tikhonenko

    Article

    Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

    Insert

    MS4A1 (MS4A1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. AOI-WT-Cas9-sg-mouse Ezh2-E18-GFP

    Plasmid

    #91880

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Ezh2 (Ezh2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pdCas9 (GB1079)

    Plasmid

    #75399

    Purpose

    Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part for C-terminal fusions

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(30-120)

    Plasmid

    #169336

    Purpose

    Expression of the LT-Fucci(SA) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Fucci(SA) (GMNN, CDT1 Human)
    

    Use

    Tags

    HaloTag7-Geminin(1/110) and HaloTag9-Cdt(30-120)

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pXJ42-p110 CUX1

    Plasmid

    #100814

    Purpose

    Plasmid vector expressing human p110 CUX1 (amino acids 878-1505 of P39880), with an N-terminal Myc tag and a C-terminal HA tag.

    Depositor

    Alain Nepveu

    Article

    Truscott et al Mol Cell Biol. 2008 May;28(10):3127-38.

    Insert

    p110 CUX1 (amino acids 878-1505) (CUX1 Human)
    

    Use

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Contains amino acids 878-1505

    Promoter

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSPL3-ABCC8-27

    Plasmid

    #135919

    Purpose

    Encodes ABCC8 exon 27 wild type for the analysis of splicing variants

    Depositor

    Diana Valverde Pérez

    Article

    Lago-Docampo et al Sci Rep. 2020 Sep 15;10(1):15135. doi: 1

    Insert

    ATP-binding Cassette subfamily C member 8 (ABCC8 Human)
    

    Use

    Exon trapping

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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