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TFORF2386

Plasmid

#143589

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MBD2 (MBD2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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CHK2_HUMAN_D0

Plasmid

#79715

Purpose

This plasmid encodes the kinase domain of CHK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

CHK2 (CHEK2 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pKLV2-U6gRNA5(hPLK1)-PGKpuro2ABFP-W

Plasmid

#163138

Purpose

Lentiviral gRNA plasmid targeting human PLK1 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

Insert

PLK1 (PLK1 Human)

Use

CRISPR and Lentiviral

Tags

BFP

Expression

Mammalian

Promoter

U6

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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DAPK1_HUMAN_D0

Plasmid

#79728

Purpose

This plasmid encodes the kinase domain of DAPK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

DAPK1 (DAPK1 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
BMPR2_HUMAN_D0

Plasmid

#79709

Purpose

This plasmid encodes the kinase domain of BMPR2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

BMPR2 (BMPR2 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
KC1E_HUMAN_D0

Plasmid

#79724

Purpose

This plasmid encodes the kinase domain of KC1E. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KC1E (CSNK1E Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
DAPK3_HUMAN_D0

Plasmid

#79721

Purpose

This plasmid encodes the kinase domain of DAPK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

DAPK3 (DAPK3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
EPHB3_HUMAN_D0

Plasmid

#79732

Purpose

This plasmid encodes the kinase domain of EPHB3. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

EPHB3 (EPHB3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
EPHA3_HUMAN_D0

Plasmid

#79708

Purpose

This plasmid encodes the kinase domain of EPHA3. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

EPHA3 (EPHA3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
EPHB2_HUMAN_D0

Plasmid

#79697

Purpose

This plasmid encodes the kinase domain of EPHB2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

EPHB2 (EPHB2 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-APLP1ICD-IRES-hrGFP

Plasmid

#107546

Purpose

AAV-mediated expression of last 50 amino acids of APLP1 protein (APLP1ICD) and IRES-mediated co-expression of hrGFP to recognize labelled cells

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

APLP1ICD (APLP1 Human)

Use

AAV

Mutation

None

Promoter

CMV

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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MK08_HUMAN_D0

Plasmid

#79695

Purpose

This plasmid encodes the kinase domain of MK08. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MK08 (MAPK8 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pUT1109-HQ858666

Plasmid

#27719

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

C2C2-CO-like transcription factor from DT643534

Use

Entry vector

Available Since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pBT2-4xUAS:P2A-mRFP

Plasmid

#127593

Purpose

This vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with mRFP through P2A self-cleaving peptides.

Depositor

Longhou Fang

Article

Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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MK11_HUMAN_D0

Plasmid

#79692

Purpose

This plasmid encodes the kinase domain of MK11. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MK11 (MAPK11 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
FES_HUMAN_D0

Plasmid

#79686

Purpose

This plasmid encodes the kinase domain of FES. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

FES (FES Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
MAPK3_HUMAN_D0

Plasmid

#79702

Purpose

This plasmid encodes the kinase domain of MAPK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MAPK3 (MAPK3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pCMV6 CHIP delU-box-Myc

Plasmid

#246738

Purpose

Mammalian expression of human CHIP with U-box domain deleted, and a myc-tag on its C-terminus

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Insert

CHIP (Carboxy terminus of Hsc70-interacting protein) (STUB1 Human)

Tags

Myc

Expression

Mammalian

Mutation

deletion of U-box domain

Promoter

CMV

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAD120-CHIP-G132N-myc-His

Plasmid

#236053

Purpose

Mammalian expression of human CHIP with the synthetic G132N substitution and C-terminal myc-His tag

Depositor

Jonathan Schisler

Article

Stewart et al bioRxiv 2025.02.17.637997

Insert

CHIP-G132N (STUB1 Human)

Tags

myc-His

Expression

Mammalian

Mutation

G132N

Promoter

CMV

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pAD000-CHIP-K30A-myc-His

Plasmid

#236052

Purpose

Mammalian expression of human CHIP with the synthetic K30A substitution and C-terminal myc-His tag

Depositor

Jonathan Schisler

Article

Stewart et al bioRxiv 2025.02.17.637997

Insert

CHIP-K30A (STUB1 Human)

Tags

myc-His

Expression

Mammalian

Mutation

K30A

Promoter

CMV

Available Since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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