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Showing: 1 - 20 of 26375 results

  14. pCR4-Dact1(1250-1601)

    Plasmid
    #52046
    Purpose
    To generate mRNA in situ probe against mouse Dact1 (Probe C in PMID 17013874). For antisense cut with Spe1, transcribe with T7. For sense cut with Not1, treat with T4 polymerase, transcribe with T3.
    Depositor
    Ben Cheyette
    Article
    Suriben et al Dev Dyn. 2006 Nov;235(11):3177-83.
    Insert
    Dact1(1250-1601) (Dact1 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  15. pCRT7 NT Topo tetR/pLtetO Amp‐WT sfGFP

    Plasmid
    #52053
    Purpose
    Used in phosphoprotein synthesis. Replace the sfGFP with gene of interest (GOI). Add TAG amber codon(s) at the position(s) in GOI where phosphoserine incorporation is desired.
    Depositor
    Jesse Rinehart
    Insert
    sfGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tetR/pLtetO
    Availability
    Academic Institutions and Nonprofits only
    52053-plasmid-map-sequence-id-80163

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  16. pKD‐SepRS-­EFSep-­5x tRNASep (B40 OTS)

    Plasmid
    #52054
    Purpose
    Used in phosphoprotein synthesis. Expresses the Sep-accepting tRNA (tRNASep), the Sep-tRNA synthetase (SepRS) and an engineered EF-Tu (EFSep)
    Depositor
    Jesse Rinehart
    Inserts
    SepRS
    EF-Sep
    5x tRNA Sep
    Use
    Tags
    Expression
    Bacterial
    Mutation
    His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…
    Promoter
    Ptac
    Availability
    Academic Institutions and Nonprofits only
    52054-plasmid-map-sequence-id-80170

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  19. PsuDJ-1.1

    Plasmid
    #52080
    Purpose
    Encodes Panagrolaimus superbus DJ-1. Potent antioxidant and molecular chaperone
    Depositor
    Ann Burnell
    Insert
    PsuDJ-1.1
    Use
    Tags
    His Tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 26375 results