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Addgene
Showing: 81 - 100 of 4370 results

  1. pKG123

    Plasmid
    #63074
    Purpose
    Retroviral expression of mCherry-TEV-S-tag CENP-C for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    CENP-C (CENPC Human)
    Use
    Retroviral
    Tags
    TEV-S and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    63074-plasmid-map-sequence-id-135907

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  2. pKG136

    Plasmid
    #63075
    Purpose
    Retroviral expression of EGFP-TEV-S-tag CENP-W for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    CENP-W (CENPW Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    E. coli codon optimized, hardened against Dharmac…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    63075-plasmid-map-sequence-id-104389

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  3. pKG186

    Plasmid
    #63076
    Purpose
    Retroviral expression of EGFP-TEV-S-tag FANC-A for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    FANC-A, IMAGE clone 5725803 (FANCA Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    63076-plasmid-map-sequence-id-104390

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  4. pKG187

    Plasmid
    #63077
    Purpose
    Retroviral expression of EGFP-TEV-S-tag FAAP24 for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    FAAP24, IMAGE clone 3659284 (FAAP24 Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    63077-plasmid-map-sequence-id-134680

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  5. pKG204

    Plasmid
    #63078
    Purpose
    Retroviral expression of EGFP-TEV-S-tag HP1 alpha for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    HP1 alpha (CBX5 Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    V151I mutation compared to GenBank reference sequ…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    63078-plasmid-map-sequence-id-134726

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  8. Flag_MmPERK_S503_N1114_pBabePuro

    Plasmid
    #58423
    Purpose
    retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant mouse PERK deleted from amino acids M1 to Y502. The remaining amino acids are S503 to N1114.
    Depositor
    David Ron
    Article
    Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11
    Insert
    PERK (Eif2ak3 Mouse)
    Use
    Retroviral
    Tags
    FLAG and preprotrypsin signal sequence
    Expression
    Mammalian
    Mutation
    Deleted amino acids M1 to Y502
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    58423-plasmid-map-sequence-id-123700

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  13. Flag_HsIRE1a_deltaP29_D408_K599A_pBabePuro

    Plasmid
    #58492
    Purpose
    retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408 and bearing mutation K599A (kinase domain mutant)
    Depositor
    David Ron
    Article
    Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11
    Insert
    IRE1a (ERN1 Human)
    Use
    Retroviral
    Tags
    FLAG and preprotrypsin signal sequence
    Expression
    Mammalian
    Mutation
    deleted amino acids P29 to D408 and bearing mutat…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    58492-plasmid-map-sequence-id-90361

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  14. HSC1-HS4-GiP

    Plasmid
    #58540
    Purpose
    Derived from HSC1-GiP where 2 copies of the HS4 insulator core are cloned inside the 3'LTR
    Depositor
    James Ellis
    Article
    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1
    Inserts
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Core HS4 insulator sequence (dimer)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human EF1-alpha
    Availability
    Academic Institutions and Nonprofits only
    58540-plasmid-map-sequence-id-90629

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  15. HSC1-HS4-D4Z4c-GiP

    Plasmid
    #58541
    Purpose
    Derived from HSC1-GiP where 2 copies of the HS4 insulator core inside the 3'LTR and the D4Z4-c insulator, are combined upstream of the EF1 alpha promoter
    Depositor
    James Ellis
    Article
    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1
    Inserts
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    D4Z4 repeat, partial
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human EF1-alpha
    Availability
    Academic Institutions and Nonprofits only
    58541-plasmid-map-sequence-id-90716

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  17. pLPNPX1

    Plasmid
    #60126
    Purpose
    expresses an insert when Cre is expressed
    Depositor
    Morris Birnbaum
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  18. pHA AKT2 T309A/S474A

    Plasmid
    #60128
    Purpose
    expresses a mutant AKT2
    Depositor
    Morris Birnbaum
    Insert
    protein kinase B (AKT2 Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mutation
    Serine 474 and Threonine 309 changed to alanines
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only

  19. pLNCX1 HA AKT2

    Plasmid
    #60129
    Purpose
    expresses HA tagged AKT2
    Depositor
    Morris Birnbaum
    Insert
    protein kinase B (AKT2 Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    60129-plasmid-map-sequence-id-96079

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Showing: 81 - 100 of 4370 results