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Plasmid#102275PurposeFor bacterial expression of His-tagged human YTHDF2DepositorInsertYTHDF2 (YTHDF2 Human)
UseTags6X HISExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BirA*-dCas9-eGFP
Plasmid#84779PurposeExpresses BirA*-dCas9-eGFP in mammalian cells.DepositorInsertBirA*-dCas9-eGFP
UseCRISPRTagscMycExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceJuly 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMRE145
Plasmid#118497Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTCF156 HLA-A*02:01
Plasmid#180456PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-A*02:01
UseTagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…PromoterAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRE147
Plasmid#118499Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmClover3
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-28c(+)-YTHDF3
Plasmid#102276PurposeFor bacterial expression of His-tagged human YTHDF3DepositorInsertYTHDF3 (YTHDF3 Human)
UseTags6X HISExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2L3
Plasmid#15795DepositorInsertUbiquitin conjugating enzyme UbE2L3 (UBE2L3 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher002
Plasmid#102827PurposeBacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteinsDepositorInsertSpyCatcher002
UseTagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBBR15.2-2H12.D11opt
Plasmid#221185PurposecdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter (Internal Lab ID: UJ11239)DepositorInsertcdGreen2.1
UseTagsExpressionBacterialMutationPromoterConstitutiveAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pMRE143
Plasmid#118495Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertsYFP2
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD1 (full-length, aa 1-481)
Plasmid#61405PurposeExpresses human OTUD1 (full-length) in E. coli.DepositorInsertOTUD1 (OTUD1 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationPromoterT7Available sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_CCT2-Halo
Plasmid#175337PurposeProduction of HaloTag fusion proteinDepositorInsertCCT2 (CCT2 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDIV303
Plasmid#204978PurposeAMA1 plasmid with Aspergillus optimized Mad7 and amdS selection markerDepositorInsertsMad7
amdS
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationtwo silent mutations (nt177T>C, nt879C>T)PromoterAspergillus nidulans tef1 promoter and Aspergillu…Available sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 hSyn1-Lck-GFP
Plasmid#177333PurposeExpresses GFP on the plasma membrane of neurons via an Lck localization sequenceDepositorInsertLck-GFP
UseAAVTagsGFPExpressionBacterialMutationPromoterhSyn1Available sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHis-TEV-Nsp15
Plasmid#187656PurposeE. coli plasmid (T7lac promoter) for full-length, wild-type SARS CoV-2 Nsp15 (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tagDepositorInsertNsp15 (N Severe acute respiratory syndrome coronavirus 2)
UseTagsTEV protease cleavable His6-tagExpressionBacterialMutationnonePromoterT7lacAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDIV301
Plasmid#204969PurposeAMA1 plasmid with Aspergillus optimized Mad7 and ble (bleomycin) resistance markerDepositorInsertsMad7
ble (bleomycin resistance marker)
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and Aspergillu…Available sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDIV300
Plasmid#204968PurposeAMA1 plasmid with Aspergillus optimized Mad7 and hph (hygromycin) resistance markerDepositorInsertsMad7
hph (hygromycin resistance marker)
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and Aspergillu…Available sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJMP12089
Plasmid#222357PurposeTn7 integrative vector with PabstBR promoter expression cassette and hygR marker on Tn7 transposon; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-cMSPdH5
Plasmid#199771PurposeExpresses split-intein fused MSPdH5 for circularization during expression (external His-Tag)DepositorInsertcMSPdH5
UseTagsHis6-TagExpressionBacterialMutationexternal His-Tags flanking the C and N terminiPromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only