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Plasmid#166763PurposeTo target Venus to the mitochondrial outer membrane. ActA tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.DepositorInsertActA tail anchor (Listeria monocytogenes) (actA Listeria monocytogenes, Synthetic)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
PJEBAN4
Plasmid#203683PurposeExpresses red fluorescent marker HcRed in Listeria monocytogenesDepositorInsertHcRed
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PJEBAN6
Plasmid#203684PurposeExpresses red fluorescent marker DsRed-Express in Listeria monocytogenesDepositorInsertDsRed-Express
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PJEBAN2
Plasmid#203681PurposeExpresses Cyan Fluorescent Protein (CFP) in Listeria monocytogenesDepositorInsertCyan Fluorescent Protein (CFP)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
PJEBAN3
Plasmid#203682PurposeExpresses Yellow Fluorescent Protein (YFP) in Listeria monocytogenesDepositorInsertYellow Fluorescent Protein (YFP)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLR16-Phes*
Plasmid#98783PurposeSuicide vector for allelic exchange in Listeria utilizing an engineered counterselection marker for Listeria monocytogenes based on a mutated phenylalanyl-tRNA synthetase gene (pheS*).DepositorInsertpheS
UseTagsExpressionBacterialMutationA309GPromoterAvailable sinceJan. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-ActA-IRES-puromycin-pLVx-EF1a
Plasmid#177433PurposeTo express mCherry fused to ActA mitochondria targeting sequence. Lentiviral vector used to make cell lines expressing this mitochondria landmark.DepositorInsertActA tail anchor (actA Listeria monocytogenes)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNF579
Plasmid#26616DepositorInsertgfpuv
UseTagsgram-positive ribosome binding siteExpressionMutationPromoterAvailable sinceFeb. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHoss1
Plasmid#63158PurposeSuicide plasmid for efficient gene mutation in Gram-positive bacteriaDepositorInsertanti-secY-Pxyl/tetO-tetR
UseSuicide plasmid for gram-positive bacteriaTagsExpressionMutationPromoterAvailable sinceSept. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCSW21
Plasmid#86836Purposebacterial expression of anti-CRISPR protein AcrIIA4DepositorInsertacrIIA4
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRAU171
Plasmid#86835Purposebacterial expression of anti-CRISPR protein AcrIIA2DepositorInsertacrIIA2
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJH372
Plasmid#86839Purposemamalian expression of AcrIIA1DepositorInsertacrIIA1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRAU168
Plasmid#86834Purposebacterial expression of anti-CRISPR protein AcrIIA1DepositorInsertacrIIA1
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCSW65
Plasmid#86838Purposebacterial expression of anti-CRISPR protein AcrIIA3DepositorInsertacrIIA3
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJH376
Plasmid#86842Purposemamalian expression of anti-CRISPR protein AcrIIA4DepositorInsertacrIIA4
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJH373
Plasmid#86840Purposemamalian expression of anti-CRISPR protein AcrIIA2DepositorInsertacrIIA2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCF525-EF1a-Hygro-P2A-AcrIIA4-lenti
Plasmid#115795PurposeExpresses AcrIIA4 in human cells, contained in a lentiviral vector.DepositorInsertAcrIIA4
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterEF-1a promoterAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTwist_Lenti_SFFV-ActA(1-584)-BFP-FKBP
Plasmid#213119PurposeExpression of an ActA FKBP payload for MinDE recruitment circuits in mammalian cellsDepositorInsertActA(1-584)-BFP-FKBP (actA Listeria monocytogenes)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
Venus-7aa-ActA-pEGFP-C1
Plasmid#177405PurposeTo express Venus fused to mitochondria outer membrane targeting sequence "ActA". Venus faces the cytosol.DepositorInsertActA tail anchor (actA Listeria monocytogenes)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-7aa-ActA-pEGFP-C1
Plasmid#177406PurposeTo express mCherry fused to mitochondria outer membrane targeting sequence "ActA". mCherry faces the cytosol.DepositorInsertActA tail anchor (actA Listeria monocytogenes)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only