Showing: 21 - 40 of 122 results
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Plasmid#112043PurposeCEN/ARS plasmid for GAL-inducible NSG2-V5-TEV-EGFP expression marked with URADepositorInsertNSG2
UseTagsV5-TEV-EGFPExpressionYeastMutationPromoterGAL1AvailabilityAcademic Institutions and Nonprofits only -
pJW1670
Plasmid#112044PurposeCEN/ARS plasmid for GAL-inducible OLE1-V5-TEV-EGFP expression marked with URADepositorInsertOLE1
UseTagsV5-TEV-EGFPExpressionYeastMutationP319TPromoterGAL1AvailabilityAcademic Institutions and Nonprofits only -
pJW1671
Plasmid#112045PurposeCEN/ARS plasmid for GAL-inducible ORM1-V5-TEV-EGFP expression marked with URADepositorInsertORM1
UseTagsV5-TEV-EGFPExpressionYeastMutationPromoterGAL1AvailabilityAcademic Institutions and Nonprofits only -
pJW1672
Plasmid#112046PurposeCEN/ARS plasmid for GAL-inducible PST1-V5-TEV-EGFP expression marked with URADepositorInsertPST1
UseTagsV5-TEV-EGFPExpressionYeastMutationPromoterGAL1AvailabilityAcademic Institutions and Nonprofits only -
pJW1673
Plasmid#112047PurposeCEN/ARS plasmid for GAL-inducible SCT1-V5-TEV-EGFP expression marked with URADepositorInsertSCT1
UseTagsV5-TEV-EGFPExpressionYeastMutationPromoterGAL1AvailabilityAcademic Institutions and Nonprofits only -
pJW1674
Plasmid#112048PurposeCEN/ARS plasmid for GAL-inducible SWP1-V5-TEV-EGFP expression marked with URADepositorInsertSWP1
UseTagsV5-TEV-EGFPExpressionYeastMutationPromoterGAL1AvailabilityAcademic Institutions and Nonprofits only -
pJW1675
Plasmid#112049PurposeCEN/ARS plasmid for GAL-inducible TSC13-V5-TEV-EGFP expression marked with URADepositorInsertTSC13
UseTagsV5-TEV-EGFPExpressionYeastMutationPromoterGAL1AvailabilityAcademic Institutions and Nonprofits only -
pJW1662
Plasmid#112050PurposeC-terminal tag with - AID*(AtIAA17_71-114)-HA and OsTIR1 expression cassette marked with LEUDepositorInsertsIAA17
TIR1
UseTagsHAExpressionYeastMutationonly amino acids 71-114PromoterADH1 and NoneAvailabilityAcademic Institutions and Nonprofits only -
pBA900
Plasmid#122237PurposeLentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs2 incorporated at the 3' end of the sgRNA constant region.DepositorInsertsgRNA with cs2 at the 3' end of the constant region
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBA904
Plasmid#122238PurposeLentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.DepositorInsertsgRNA with cs1 in stem loop 2 of the constant region
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBA950
Plasmid#122239PurposeCROP-seq vector optimized for sgRNA expression and CRISPRi activity. This vector expresses a eGFP-NT2 sgRNA with modified U6 promotor and sgRNA constant region.DepositorInsertBFP selectable marker, plus a modified mU6 promoter and sgRNA constant region for optimized CRISPRi activity
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-KRAB-dCas9-P2A-mCherry
Plasmid#60954Purpose2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherryDepositorInsertKRAB-dCas9-P2A-mCherry fusion
UseCRISPR and LentiviralTags2x NLS, HA, KRAB domain, and mCherryExpressionMammalianMutationPromoterSFFVAvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP
Plasmid#60955PurposeParental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoterDepositorInsertssgGFP-NT2
puro-T2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJR85
Plasmid#140095PurposeLentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.DepositorInsertsgRNA with cs1 in stem loop 2 of the constant region
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJR89
Plasmid#140096PurposesgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.DepositorInsertsgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
UseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
PCT62
Plasmid#170391Purposeexpresses three sgRNA cassettesDepositorInsertPCT62-targetsite
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pMH0004
Plasmid#174087PurposeUCOE-SFFV-Cas9-BFPDepositorInsertCas9-BFP
UseLentiviralTagsExpressionMutationPromoterSFFVAvailabilityAcademic Institutions and Nonprofits only -
pMH0007
Plasmid#174162PurposeUCOE-EF1A-Cas9-BFPDepositorInsertCas9-BFP
UseLentiviralTagsExpressionMutationPromoterEF1AAvailabilityAcademic Institutions and Nonprofits only -
pJW1504
Plasmid#62358PurposeRPS2 HA-TEV-AVI pop-in/pop-out plasmidDepositorInsertRPS2 fragment
UseYeast targetingTagsHA-TEV-AVIExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJW1505
Plasmid#62359PurposeRPL16a HA-TEV-AVI pop-in/pop-out plasmidDepositorInsertRPL16A
UseYeast taggingTagsHA-TEV-AVIExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 21 - 40 of 122 results